Sugar Tech

, Volume 4, Issue 1–2, pp 7–13 | Cite as

Studies on possibility of using ICUMSA microbiological pour plate methods for refined sugars for enumeration of microbial populations in primary and mixed sugarcane juice

  • S. Kumar
  • P. K. Agrawal
Sugar Tech


The studies have been carried out to examine the possibility of determining the microbial densities present in sugarcane primary and mixed juice by using the existing ICUMSA pour plate methods, GS - 2/3 - 43 to 49 (1994) developed for counting different types of microorganisms in refined sugar, and also to ascertain if the microbiological growth media referred in these methods could be used as such or after some modification. The results suggest that the existing ICUMSA microbiological pour plate methods for refined sugar could be used for sugarcane juice microbiological analyses also without any major modification, though the samples would have to be suitably diluted prior to plating, say 10, 000 to ten million times, depending on the microbial cells densities present therein. Further, the growth media containing crude extracts like yeast extract, beef extract, peptone, etc. supported better microbial growth than the chemically defined ones, like Weman medium.

Key Words

ICUMSA microorganisms sugarcane juice mesophiles slime formers yeasts & molds thermophiles 


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  1. Bevan, D. and Bond, J. (1971). Microorganisms in field — A Preliminary Survey. Proc. QSSCT, p. 137.Google Scholar
  2. Irvine, P.S. (1980). Proc. CSRRP, p. 116–120.Google Scholar
  3. Wells, W.D. and James, G.P. (1976). Rapid “Dextrans” Formation in Stale Cane and its Processing Consequences. Proc. QSSCT, p. 287.Google Scholar
  4. Foster, C.S. (1980). Proc. ISSCT, p. 2204–2220.Google Scholar
  5. Foster, D.H., Ivin, P.C. and King, H. (1977). Measurements of the Deterioration of Green and Burnt Mechanically Harvested Cane. Proc. QSSCT, p. 37.Google Scholar
  6. Shahata, P. S. K. (1960). J. Appl. Microbiol. 8, p. 17.Google Scholar
  7. Chen, J. C. P. (1964). Preliminary Investigation on the Presence of Osmophillic Yeasts in Mauritian Raw Sugar. Int. Sugar J.,27: 666.Google Scholar
  8. Madaree, S. J., Mackray, L. M. and Coulthard, G. N. (1971). Proc. South African Sugar Tech. Assoc, p. 135–138.Google Scholar
  9. Shore, M., Adams, J.A., Broughton, N.W. and Parslow, R. (1982). Rapid Determination of Lactic Acid in Factory Juices using Gas Chromatography. Int. Sugar J.,84: 163.Google Scholar
  10. ICUMSA Methods Book (1994). Pub. by British Tech. Centre, U.K.Google Scholar
  11. Stanier, R.Y., Duodoroff, M. and Adelberg, M. (1976). General Microbiology, Pub. by Prentice Hall Inc., N.Y., U.S.A., p. 306.Google Scholar

Copyright information

© Society for Sugar Research & Promotion 2002

Authors and Affiliations

  • S. Kumar
    • 1
  • P. K. Agrawal
    • 1
  1. 1.Biochemistry DivisionNational Sugar InstituteKanpur - 208 017, UPIndia

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