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A dynamic study of the cytotoxic effects of hyperthermia combined with cis-diamine dichlorolplatinum (DDP) on human gastric cancer cell lines MKN28 and MKN45 in vitro

  • Clinical Observations
  • Published:
Chinese Journal of Cancer Research

Abstract

Cytotoxic effects of hyperthermia combined with DDP on MKN28 and MKN45 cells were studied by MTT assay according to a nested design. The results showed: hyperthermia alone above 43 °C for 30 mins was cytotoxic; hyperthermia at temperature lower than 43 °C for 30 mins could increase sensitivity of cancer cells to DDP. The cytotoxic effect of simultaneous use of hyperthermia and DDP was more marked than that of sequential use of the 2 treatments. Hyperthermia combined with DDP could inhibit growth of human gastric adenocarcinoma cells regardless of their degree of differentiation.

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References

  1. Jiao H, Shen W, Ohe Y, et al. A new 3- (4,5-dimethylthiazol-- 2--yl) --2. 5--diphenyltetrazolium bromide (MTT) assay for testing macrophage cytotoxicity to L1210 and its drug resistant cell linesin vitro. Cancer Immunol Immunother 1992; 35: 412.

    Article  PubMed  CAS  Google Scholar 

  2. Kobayashi K, Fujimoto S, Takahashi M, et al. The experimental and clinical study of hyperthermia with thermosensitizer for gastric cancer patients with peritoneal seeding. Can To Kagaku Ryoho 1992; 19: 1651.

    CAS  Google Scholar 

  3. Kent EW, Michael WD, Gloria CL. Hyperthermic potentatiation of cis-diaminedichloroplatnum (II) cytotoxicity in Chinese hamster over cells resistant to the drug. Cancer Res 1986; 46: 6242.

    Google Scholar 

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Authors and Affiliations

  1. Department of Gastroenterology, First Affiliated hospital, Zhejiang Medical University, 310003, Hangzhou

    Chen Weixing, Li Youming, Chen Liangliang, Ji Feng, Huang Huaide & Liu Xianglin

  2. Department of Oncology, Chinese Traditional Medical hospital of Zhejiang, China

    Chen Liangliang

Authors
  1. Chen Weixing
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  2. Li Youming
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  3. Chen Liangliang
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  4. Ji Feng
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  5. Huang Huaide
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  6. Liu Xianglin
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Chen, W., Li, Y., Chen, L. et al. A dynamic study of the cytotoxic effects of hyperthermia combined with cis-diamine dichlorolplatinum (DDP) on human gastric cancer cell lines MKN28 and MKN45 in vitro . Chinese Journal of Cancer Research 8, 298–300 (1996). https://doi.org/10.1007/BF02954755

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  • DOI: https://doi.org/10.1007/BF02954755

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