Abstract
The cervical carcinoma cell line, ME180 cells were treated with tanshinone (Tan) or retinoic acid (RA) in DMSO (final concentration 0.02%, V/V) on 4 successive days. The cells treated with the same concentration of DMSO alone served as control. Morphological studies with light and transmission electron microscopy showed that the cells treated with both Tan and RA became well-differentiated. The cellular growth and proliferation were suppressed (as revealed by cell counting. [3H]-thymidine uptake and colony-forming assay). The number of nuclear organizer regions (AgNORs) in cells reduced and the distribution type returned nearly to normal type. The tumorigenicity in nude mice was reduced. The cell RNA dot hybridization showed that the expression of c-myc and c-Ha-ras mRNA was inhibited markedly. All above results showed that Tan and RA could reverse some malignant phenotype and possessed differentiation inducing activity on ME180 cell line. No significant difference was observed between the cells treated with Tan and RA.
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Chang WL, Chen CF. Cytotoxic activities of tanshinone against human carcinoma cell lines. Am J China med 1991; 19(3–4): 207.
Sykes JA, whitescaver J, Jernstrom P, et al. Some properties of a new epithelial cell line of human origin. J Natl Cancer Inst 1970; 45: 107.
Crocker J, Nar P. Nucleolar organizer regions in lymphomas. J Pathol 1987; 151:11.
Conway K, Morgan D, Phillips KK, et al. Tumorigenic suppression of a human cutaneous squamous cell carcinoma cell line in the nude mouse skin graft assay. Cancer Res 1992; 1152:6487.
Paeratakul U, De-Stasio PR, Taylor MC, et al. A first and sensitive method for detecting specific viral RNA in mammalian cell. J Viral 1988; 62: 1131.
Stinson SF, alley MC, kopp WC, et al. Morphological and immunocytochemical characteristics of human tumor cell line for use in disease oriented anticancer drug screen. Anti Cancer Res 1992; 12:1035.
Ai ZW, Zha XL, chen HL. Effect of retinoic acid on hepatocarcinoma cell line. J Tumor Marker Oncol 1990; 328.
Loodwood AH, Murphy SK, Borislow. Cellular signal transduction and the reveral of malignancy. J Cell Biochem 1987; 33:237.
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This work was supported by the Science Fund of the Ministry of Public Health.
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Yuan, S., Huang, G., Wang, X. et al. The differentiation inducing effect of tanshinone and retinoic acid on human cervical carcinoma cell line (ME180)in vitro . Chinese Journal of Cancer Research 8, 244–248 (1996). https://doi.org/10.1007/BF02954740
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DOI: https://doi.org/10.1007/BF02954740