Abstract
Abnormally elevated activity of ornithine decarboxylase (ODC), and subsequent polyamine accumulation are intimately associated with the genesis, development and metastasis of cancer. In the present study, to control the growth of tumor cells, ODC antisense RNA was used to transfect human lung squamous carcinoma cell line LTEP-78. Compared with the parental cells, growth of the antisense transfected LTEP-78 cells arrested in G0/G1 phase and colony formation in soft agarose and tumorigenicity in nude mice were significantly reduced. Nucleic acid hybridization demonstrated that the transfectants expressed a high level of ODC antisense RNA and a significantly reduced level of endogenous ODC mRNA. The results suggest that the reversion of malignant phenotypes of human lung squamous carcinoma cells transfected with ODC antisense RNA is associated with the inhibition of polyamine biosynthesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Clifford A, Morgan D, Yuspa SH, et al. Role of ornithine decarboxylase in epidermal tumorigenesis. Cancer Res 1995; 55: 1680.
Hollta E, Sistonen L, Alitalo K. The mechanisms of ornithine decarboxylase deregulation in c-Ha-ras oncogene-transformed NIH3T3 cells. J Biol Chem 1988; 263: 4500.
Hibshoosh H, Johnson M, Weinstein IB. Effects of overexpression of ornithine decarboxylase (ODC) on growth control and oncogene-induced cell transformation. Oncogene 1991; 6: 739.
Moshier J, Dosescu J, Skunca M, et al. Transformation of NIH3T3 cells by ornithine decarboxylase overexpression. Cancer Res 1993; 53: 2618.
Auvinen M, Paasinen A, Andersson LC, et al. Ornithine decarboxylase activity is critical for cell transformation. Nature 1992; 360: 355.
Basu HS, Sturkenoboom MCJM, Delcros Jg, et al. Effect of polyamine depletion on chromatin structure in U-87 MG human brain tumor cells. Biochem J 1992; 282: 723.
Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1989.
Pegg AE. Polyamine metabolism and its importance in neoplastic growth as a target for chemotherapy. Cancer Res 1988; 48: 759.
Quemener V, Blanchard Y, Chamaillard L, et al. Polyamine deprivation: A new tool in cancer treatment. Anticancer Res 1994; 14: 443.
Author information
Authors and Affiliations
Additional information
This research was supported by grant from National Natural science foundation of China.
Rights and permissions
About this article
Cite this article
Guan, J., Fan, M. & Cao, S. Reversion of malignant phenotypes of human lung squamous carcinoma cells by ornithine decarboxylase antisense RNA. Chinese Journal of Cancer Research 8, 240–243 (1996). https://doi.org/10.1007/BF02954739
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02954739