Mechanism of the effect of guzhen recipe on regulating immune function in senile rats
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Objective: To explore the effect of Guzhen recipe, GZR) on mRNA expression of interleukin-2 (IL-2) and interleukin-6 (IL-6) in senile rats as well as the relationship between dehydroepi-androsterone (DHEA) and its sulfate (DHEAS) and the immune function regulation of GZR.Methods: Northern Blot and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the mRNA expression of IL-2 and IL-6, meanwhile coating-antibody-counting method was used to detect DHEA(S) level in serum.Results: Compared with the young group, the splenocyte mRNA expression of IL-2 in senile rats was lowered and that of IL-6 was increased along with the decrease of serum DHEA(S) level. GZR, however, could improve the above indices in the senile rats.Conclusion: GZR might regulate the immune function of aged rats through increasing the serum level of DHEA(S).
Key WordsGuzhen recipe interleukin-2 mRNA interleukin-6 mRNA dehydroepiandrosterone (sulfate)
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