An XRF study of trace elements accumulation in kidneys of tumor-bearing mice after treatment withcis-DDP with and without selenite and selenocistamine

  • Cesia Shenberg
  • Miriam Boazi
  • Jacob Cohen
  • Abraham Klein
  • Marcus Kojler
  • Abraham Nyska


The effect ofcis-DDP treatment with and without selenite and selenocistamine was studied on kidneys of tumor-bearing mice. The amounts ofcis-DDP, selenite, and selenocistamine injected were chosen so as to be compatible with the treatment of humans. The animals were sacrificed at 7, 14, and 28 d after treatment. The kidneys were removed and subjected to trace element analysis by a novel X-ray fluorescence (XRF) method and for pathological assessment. The results show that following treatment withcis-DDP, K, Fe, Cu, and Zn reach a maximum level after 7 d; K, Fe, and Cu levels were significantly reduced by the addition of selenite. The level of Zn was reduced only in groups treated with selenite whereas that of K and Cu was reduced also in groups treated with selenocistamine with and withoutcis-DDP. The greatest increase in Pt and Se levels was reached 1 wk after injection withcis-DDP, with and without selenocompounds, and in the case of Pt was partly reduced by addition of selenite. Se returned to control values 2 wk after injection, although Pt was still high in all groups 2 and 4 wk after injection. The results corroborate the findings of our previous studies. The effect of selenocistamine incis-DDP treated mice was partly insufficient. The pathological examination of the kidneys did not show any differences in the effect of various additives during the study.

Index Entries

Trace elements XRF mouse kidney cis-DDP selenite, selenocistamine 


  1. 1.
    C. F. J. Barnard, M. J. Cleare, and P. C. Hydes,Chemistry in Britain, November,22, 1001–1004 (1986).Google Scholar
  2. 2.
    S. G. Carvajal, R. V. Citarella, E. F. Delos Santos, F. E. Durr, J. J. Hlavka, S. A. Lang Jr., H. L. Lindsay, J. P. Thomas, R. E. Wallace, and Yand-i-Lin,J. Med. Chem.,32, 2063–2067 (1989).PubMedCrossRefGoogle Scholar
  3. 3.
    J. P. Berry, C. Pauwells, S. Tlouzean, and G. Lespinats,Cancer Res.,44, 2864–2868 (1984).PubMedGoogle Scholar
  4. 4.
    K. Ohkawa, Y. Tsukada, H. Dohzono, K. Koike, and Y. Terashima,Br. J. Cancer 58, 38–41 (1988).PubMedGoogle Scholar
  5. 5.
    M. Satoh, A. Naganuma, and N. Imura,J. Pharmacobio. Dyn.,12, 246–253 (1989).PubMedGoogle Scholar
  6. 6.
    Yoshikazu Araya,Hokkaido Igaku Zasshi,65, 245–253 (1990).PubMedGoogle Scholar
  7. 7.
    C. Shenberg, T. Izak-Biran, M. Mantel, B. Rachmiel, J. Weininger, and S. Chaitchik,J. Trace Elem. Electrolytes Health Dis.,3, 71–75 (1989).PubMedGoogle Scholar
  8. 8.
    J. P. Berry and G. Lespinats,J. Submicrosc. Cytol. Pathol.,20, 59–65 (1988).PubMedGoogle Scholar
  9. 9.
    H. Sakurai, K. Tsuchiya, S. Hirai, Y. Okada, and H. Haraguchi,Natur-wissenschaften,75, 405–406 (1988).CrossRefGoogle Scholar
  10. 10.
    Glenn S. Baldew, J. Gordon McVie, Martin A. van der Valk, Gerrit Los, Jeroen J. M. De Goey, and Nico P. E. VermeulenCancer Res.,50, 7031–7036 (1990).PubMedGoogle Scholar
  11. 11.
    C. Shenberg, A. Klein, T. Izak-Biran, M. Mantel, and S. Chaitchik, inFifth Int. Symp. on Selenium in Biology and Medicine, Abstract Book, Nashville, TN, 1992, p. 109.Google Scholar
  12. 12.
    G. C. Guidi, G. Bellisola, G. Bonadonna, F. Manzato, O. Ruzzenente, R. Schiavon, S. Galassini, Q. X. Liu, H. R. Shao, G. Moschini, and G. Perona,J. Trace Elem. Electrolytes Health Dis. 4, 157–161 (1990).PubMedGoogle Scholar
  13. 13.
    R. Hay, J. Caputo, T. R. Chen, M. Macy, P. Meclintock, and Y. Reid, eds.,American Type Collection Catalog of Cell Lines and Hybridomas, 7th ed., 1992.Google Scholar
  14. 14.
    R. J. Geran, N. H. Greenberg, M. M. Mackdonald, A. M. Schumacher, and B. J. Abbot,Cancer Chemotherapy Reports, Part 3, vol. 3, 1972, pp. 7–87.Google Scholar

Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Cesia Shenberg
    • 1
  • Miriam Boazi
    • 1
  • Jacob Cohen
    • 1
  • Abraham Klein
    • 2
  • Marcus Kojler
    • 2
  • Abraham Nyska
    • 3
  1. 1.Soreq Nuclear Research CentreYavneIsrael
  2. 2.Department of Life SciencesBar-Ilan UniversityRamat-GanIsrael
  3. 3.Kimron Veterinary InstituteBeit-DaganIsrael

Personalised recommendations