An XRF study of trace elements accumulation in kidneys of tumor-bearing mice after treatment withcis-DDP with and without selenite and selenocistamine
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The effect ofcis-DDP treatment with and without selenite and selenocistamine was studied on kidneys of tumor-bearing mice. The amounts ofcis-DDP, selenite, and selenocistamine injected were chosen so as to be compatible with the treatment of humans. The animals were sacrificed at 7, 14, and 28 d after treatment. The kidneys were removed and subjected to trace element analysis by a novel X-ray fluorescence (XRF) method and for pathological assessment. The results show that following treatment withcis-DDP, K, Fe, Cu, and Zn reach a maximum level after 7 d; K, Fe, and Cu levels were significantly reduced by the addition of selenite. The level of Zn was reduced only in groups treated with selenite whereas that of K and Cu was reduced also in groups treated with selenocistamine with and withoutcis-DDP. The greatest increase in Pt and Se levels was reached 1 wk after injection withcis-DDP, with and without selenocompounds, and in the case of Pt was partly reduced by addition of selenite. Se returned to control values 2 wk after injection, although Pt was still high in all groups 2 and 4 wk after injection. The results corroborate the findings of our previous studies. The effect of selenocistamine incis-DDP treated mice was partly insufficient. The pathological examination of the kidneys did not show any differences in the effect of various additives during the study.
Index EntriesTrace elements XRF mouse kidney cis-DDP selenite, selenocistamine
- 1.C. F. J. Barnard, M. J. Cleare, and P. C. Hydes,Chemistry in Britain, November,22, 1001–1004 (1986).Google Scholar
- 11.C. Shenberg, A. Klein, T. Izak-Biran, M. Mantel, and S. Chaitchik, inFifth Int. Symp. on Selenium in Biology and Medicine, Abstract Book, Nashville, TN, 1992, p. 109.Google Scholar
- 13.R. Hay, J. Caputo, T. R. Chen, M. Macy, P. Meclintock, and Y. Reid, eds.,American Type Collection Catalog of Cell Lines and Hybridomas, 7th ed., 1992.Google Scholar
- 14.R. J. Geran, N. H. Greenberg, M. M. Mackdonald, A. M. Schumacher, and B. J. Abbot,Cancer Chemotherapy Reports, Part 3, vol. 3, 1972, pp. 7–87.Google Scholar