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Applied Biochemistry and Biotechnology

, Volume 36, Issue 1, pp 75–80 | Cite as

A simple method for detection of monoclonal isotypes

  • Y. M. Gomes
  • L. N. Regis
  • A. B. Carvalho
  • M. Nakazawa
  • L. Monjour
  • A. F. Furtado
Article

Abstract

A simple rapid method of enzyme labeled anti-isotype assay (ELIA) for detection of monoclonal isotype on hybridoma cells is proposed. This alternative method was first carried out on hybridoma cell lines 147C11 and 257C11 produced againstTrypanosoma cruzi and male accessory secretion ofPanstrongylus megistus, respectively. The monoclonal antibodies produced by these hybridoma were characterized by this method as IgM (147C11) and IgGl (257C23) isotypes, allowing evaluation of isotype without having to wait until the concentration of antibody present in the supernatant itself rises. Results were confirmed by Ouchterlony immunodiffusion. The proposed method offers the advantages of a permanent rapid procedure for light microscopy.

Index Entries

Monoclonal isotypes characterization of monoclonal isotypes monoclonal antibody 

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Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Y. M. Gomes
    • 1
  • L. N. Regis
    • 1
    • 2
  • A. B. Carvalho
    • 1
  • M. Nakazawa
    • 1
  • L. Monjour
    • 3
  • A. F. Furtado
    • 1
  1. 1.Departamento de ImunologiaCentro de Pesquisas Aggeu Magalhāes-FIOCRCIZRecife-PEBrazil
  2. 2.Departamento de ZoologiaUniuersidade Federal de Pemambuco-UFPERecife-PEBrazil
  3. 3.Service de Parasitologie et Médecine Tropicale, Groupe Hospitaller, Pitié-SalpātrièreINSERM U 313, 91ParisFrance

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