Applied Biochemistry and Biotechnology

, Volume 36, Issue 1, pp 75–80 | Cite as

A simple method for detection of monoclonal isotypes

  • Y. M. Gomes
  • L. N. Regis
  • A. B. Carvalho
  • M. Nakazawa
  • L. Monjour
  • A. F. Furtado


A simple rapid method of enzyme labeled anti-isotype assay (ELIA) for detection of monoclonal isotype on hybridoma cells is proposed. This alternative method was first carried out on hybridoma cell lines 147C11 and 257C11 produced againstTrypanosoma cruzi and male accessory secretion ofPanstrongylus megistus, respectively. The monoclonal antibodies produced by these hybridoma were characterized by this method as IgM (147C11) and IgGl (257C23) isotypes, allowing evaluation of isotype without having to wait until the concentration of antibody present in the supernatant itself rises. Results were confirmed by Ouchterlony immunodiffusion. The proposed method offers the advantages of a permanent rapid procedure for light microscopy.

Index Entries

Monoclonal isotypes characterization of monoclonal isotypes monoclonal antibody 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kohler, G. and Milstein, C. (1975),Nature 256, 256–497.CrossRefGoogle Scholar
  2. 2.
    Snary, D., Ferguson, M. A. J., Scott, M. T., and Allen, A. K. (1981),Biochem. Parasitol. 3 343–356.CrossRefGoogle Scholar
  3. 3.
    Monjour, L., Berneman, A., Vouldoukis, I., Domurado, M., Guillemin, M.C., Chopin, C., Alfred, C., and Roseto, A. (1985),C. R. Acad. Sc. Paris 300, 395–398.Google Scholar
  4. 4.
    Kresina, T. F. and Olds, R. (1989),J. Clin. Invest. 83, 912–920.CrossRefGoogle Scholar
  5. 5.
    Ruiz, A. M., Esteva, M., Subias, E., Moreno, M., Rosenstein de Campanini, A., Velazquez, E., and Segura, E. L. (1990),Mol. Biochem. Parasitol. 39, 117–126.CrossRefGoogle Scholar
  6. 6.
    Nisonoff, A. (1982), InIntroduction to Molecular Immunology, Sinauer Associates, Inc., Sunderland, MA, pp. 161–172.Google Scholar
  7. 7.
    Ouchterlony, O. (1958), InProgress in Allergy (Kahlos, P., ed.), Karger, Basel, vol. 5, pp. 1–78.Google Scholar
  8. 8.
    Voller, A. (1975),The Lancet 22, 426–428.CrossRefGoogle Scholar
  9. 9.
    Coons, A. H. and Kaplan, M. M. (1950),J. Exper. Med. 91, 1–13.CrossRefGoogle Scholar
  10. 10.
    Schulman, M., Wilde, C. D., and Kohler, G. (1978),Nature 276, 269–270.CrossRefGoogle Scholar
  11. 11.
    Gomes, Y. M., Debons-Guillemin, M. C, Regis, L. N., Furtado, A. F., and Monjour, L. (1991),Mem. Inst. Oswaldo Cruz (Suppl. I)86, 229.Google Scholar
  12. 12.
    Regis, L. N., Furtado, A. F., Gomes, Y. M., and Monjour, L. (1990),Mem. Inst. Oswaldo Cruz (Suppl. I)85, 111.Google Scholar
  13. 13.
    Bourne, J. A. (1983), InHandbook of Imtnunoperoxidase Staining Methods, Immunochemistry Laboratory DAKO Corporation, pp. 6–37.Google Scholar

Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Y. M. Gomes
    • 1
  • L. N. Regis
    • 1
    • 2
  • A. B. Carvalho
    • 1
  • M. Nakazawa
    • 1
  • L. Monjour
    • 3
  • A. F. Furtado
    • 1
  1. 1.Departamento de ImunologiaCentro de Pesquisas Aggeu Magalhāes-FIOCRCIZRecife-PEBrazil
  2. 2.Departamento de ZoologiaUniuersidade Federal de Pemambuco-UFPERecife-PEBrazil
  3. 3.Service de Parasitologie et Médecine Tropicale, Groupe Hospitaller, Pitié-SalpātrièreINSERM U 313, 91ParisFrance

Personalised recommendations