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Routine immunofluorescent microscopy in renal diseases

  • A. H. Nagi
Article
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Summary

Immunoflourescent microscopy has been used as a routine method in the diagnosis of renal biopsies from 128 patients. Positive fluorescence of immunoglobulins was obtained in the majority of renal glomerular diseases with the exception of focal glomerular sclerosis and the renal lesion of Henoch-Schonlein’s syndrome, in which no fluorescence was obtained. On the basis of distribution pattern of immunoglobuiins it has been suggested that fluorescent antibody tests when used in combination with the usual histological examinations, may be an important differential diagnostic tool, and may help in understanding the pathogenesis of various renal diseases of unknown aetiology.

Keywords

Glomerulonephritis Lupus Nephritis Renal Biopsy Glomerular Basement Membrane Membranous Nephropathy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Andres, G. A. L., Accinni, L., Hsu, K. C., Zabriskie, J. B., Seegal, B. C. 1966. Electron microscopic studies of human glomerulonephritis with ferritin conjugated antibody. J. Exp. Med. 123, 399.PubMedCrossRefGoogle Scholar
  2. Barabas, A. Z., Nagi, A. H. and Lannigan, R. 1970. Induction of autologous immune-complex nephritis in rats by heterologous anti-kidney mitochondrial antisrum. Inter. Urol. Neph. 2, 303.CrossRefGoogle Scholar
  3. Dixon, F. J. 1968. The pathogenesis of glomerulonephritis. Amer. J. Med. 44, 493.PubMedCrossRefGoogle Scholar
  4. Earle, D. P. and Jennings, R. B. 1961. Post-streptococcal glomerulonephritis. In Wolsten-holme, G. E. W. and Cameron, M. P. (Eds.), Ciba Foundation symposium on renal biopsy. Boston, Little, Brown, p. 156.CrossRefGoogle Scholar
  5. Edgington, T. S., Glassock, R. J. and Dixon, F. J. 1967. Autologous immune-complex pathogenesis of experimental allergic glomerulonephritis. Science. 155, 1432.PubMedCrossRefGoogle Scholar
  6. Friedman, P., Peters, J. H. and Kark, R. M. 1960. Localisation of gamma-globulin in diseased kidney. Arch. Intern. Med. 105, 524.Google Scholar
  7. Hardwicke, J. 1971. Immunological mechanisms in glomerulonephritis. J. Roy. Coll. Phys. Lond. 5, 140.Google Scholar
  8. Herdman, R. C. Pickering, R. J., Michael, A. F., Vernier, R. L., Fish, A. J., Gewurz, H. and Good, R. A. 1970. Chronic glomerulonephritis associated with low serum complement activity (chronic hypocomplementemic glomerulonephritis). Medicine, 49, 207.PubMedCrossRefGoogle Scholar
  9. Heymann, W., Hackle, D. B., Harwood, S., Wilson, S. G. E. and Hunter, J. L. P. 1959. Production of nephrotic syndrome in rats by Freund’s adjuvant and rat kidney suspension. Proc. Soc. Exper. Biol. Med. 100, 660.Google Scholar
  10. Heymann, W., Grupe, W. E., Hunter, J. L. P., Camma, A., Hackle, D. B., Cuppage, F. E. and Weinberg, A. G. 1970. Production of experimental autoimmune nephrosis in rats by heterologous tissues. Nephron. 7, 363.CrossRefGoogle Scholar
  11. Hauba, V., Alison, A. C., Hendrickse, R. G., de Petris, S., Edington, G. M. and Adeniyi, A. Immune complexes in nephrotic syndrome of Nigerian children. In Proceedings of the International Symposium on Immune Complex Diseases. Bonomo, L. and Turk, J. L. (Eds.). Carlo Erba Foundation. Milan, Italy. p. 23-35.Google Scholar
  12. Kaplan, M. H. 1958. Localisation of streptococcal antigens in tissues. I. Histologic distribution and persistence of M proteins, types 1, 5, 12 and 19 in tissues of mouse. J. Exp. Med. 107, 341.PubMedCrossRefGoogle Scholar
  13. Lange, K., Treser, G., Sagal, I., Ty, A. and Wasserman, E. 1966. Routine immunohistology in renal diseases. Amer. J. Intern Med. 64, 25.Google Scholar
  14. Lawrence, J. R., Pollak, V. E., Pirani, C. L. and Kark, R. M. 1963. Histologic and clinical evidence of poststreptococcal glomerulonephritis in patients with the nephrotic syndrome. Medicine, 42, 1.CrossRefGoogle Scholar
  15. Lewis, E. J. and Couser, W. G. 1971. The immunologic basis of human renal disease. Pediat. Clin. North Amer. 18(2), 467.Google Scholar
  16. McCluskey, R. T. and Baldwin, D. S. 1963. Natural history of acute glomerulonephritis. Amer. J. Med. 35, 213.PubMedCrossRefGoogle Scholar
  17. McCluskey, R. T., Vassalli, P., Gallo, G. and Baldwin, D. S. 1966. An immunofluorescent study of pathogenic mechanisms in glomerular diseases. New Eng. J. Med. 274, 695.PubMedGoogle Scholar
  18. Markowitz, A. S. and Lange, C. F. 1964. Streptococcal related glomerulonephritis. I. Isolation, immunochemistry and comperetive chemistry of soluble fractions from type 12 nephritogenic streptococci and human glomeruli. J. Immunol. 92, 565.PubMedGoogle Scholar
  19. Markowitz, A. S. 1969. Streptococcal related glomerulonephritis in the rhesus monkey. Transplant Proc. 1, 985.PubMedGoogle Scholar
  20. Mellors, R. C. and Ortega, L. G. 1956. Analytical pathology. III. New observations on the pathogenesis of glomerulonephritis, lipid nephrosis, periarteritis nodosa and secondary amyloidosis in man. Amer. J. Path. 32, 455.PubMedGoogle Scholar
  21. Michael, A. F., Drummond, K. N., Vernier, R. L. and Good, R. A. Immunologic basis of renal disease. Pediat. Clin. N. Amer. 11, 685.Google Scholar
  22. Millar, F. 1961. Renal localisation and persistence of type 12 streptococcal M-protein. Proc. Soc. Exp. Biol. Med. 108, 539.Google Scholar
  23. Nagi, A. H. 1971a. The nephrotic syndrome—human and experimental observations. A thesis accepted for Ph.D. by the Queen’s University, Belfast. p. 73.Google Scholar
  24. Nagi, A. H. 1971b. Histological, ultrastructural and immunofluorescent studies in mem-branoproliferative glomerulonephritis. J. Path. 106, 151.CrossRefGoogle Scholar
  25. Nagi, A. H., Alexander, F. and Lannigan, R. 1971. Focal glomerular sclerosis—Light and electron microscopical studies. J. Clin. Path. 24, 845.CrossRefGoogle Scholar
  26. Nagi, A. H., Barabas, A. Z., Alexander, F. and Lannigan, R. 1971. Induction of progressive glomerulonephritis in rats by eluted gamma-globulin. Brit. J. Exp. Path. 52, 371.PubMedGoogle Scholar
  27. Stebley, R. W. 1962. Glomerulonephritis induced in sheep by injections of heterologous glomerular basement membrane and Freund’s complete adjuvant. J. Exp. Med. 116, 252.Google Scholar
  28. Stebley, R. W. 1963. Glomerulonephritis induced in sheep and monkeys by injections of heterologous glomeruloar basement membrane and Freund’s adjuvant. In Graber, P. and Miescher, P. A. (eds). 3rd international symposium on Immunopathology. Basel, Schwabe and Co. p. 252–262.Google Scholar
  29. Treser, G., Seinar, M., McVicar, M., Franklin, M., Ty, A., Sagel, I. and Lange, K. 1969. Antigenic streptococcal components in acute glomerulonephritis. Science, 163, 676.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 1973

Authors and Affiliations

  • A. H. Nagi
    • 1
  1. 1.Department of PathologyQueen’s University of BelfastBelfastN. Ireland

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