Biotechnology and Bioprocess Engineering

, Volume 9, Issue 1, pp 62–64 | Cite as

Biocatalytic preparation of chiral epichlorohydrins using recombinantPichia pastoris expressing epoxide hydrolase ofRhodotorula glutinis

  • Hee Sook Kim
  • Jae-Hwa Lee
  • Sunghoon Park
  • Eun Yeol Lee


The use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinantPichia pastoris with the enantioselective epoxide hydrolase ofRhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5% (v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100%ee and a yield of 26% were obtained within 5 min from 50 mM racemates.


enantioselective hydrolysis epichlorohydrin epoxide hydrolase Rhodotorula glutinis Pichia pastoris 


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Copyright information

© KSBB 2004

Authors and Affiliations

  • Hee Sook Kim
    • 1
  • Jae-Hwa Lee
    • 2
  • Sunghoon Park
    • 3
  • Eun Yeol Lee
    • 1
  1. 1.Department of Food Science and TechnologyKyungsung UniversityBusanKorea
  2. 2.Department of Bioscience and BiotechnologySilla UniversityBusanKorea
  3. 3.Department of Chemical and Biochemical EngineeringPusan National UniversityBusanKorea

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