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Chinese Journal of Cancer Research

, Volume 10, Issue 3, pp 178–181 | Cite as

Adenovirus-mediated wild-type P53 expression suppresses growth of lung adeno-carcinoma cells

  • Li Jian 
  • Xia Yongjing 
  • Jiang Lei 
  • Li Hongxia 
  • Hu Yajun 
  • Yi Lin 
  • Hu Shixue 
  • Xu Hongji 
Basic Investigations

Abstract

Objective: To study the growth suppression of lung adenocarcinoma cell by the introduction of wild-type P53 gene and explore a gene therapy approach for lung adenocarcinoma. Methods: A replication-deficient adenovirus vector encoding a wild-type P53 was constructed and transfected into the cultured human lung adenocarcinoma cell line GLC-82. The efficiency of gene transfection and expression was detected by immunochemical staining and polymerase chain reaction. The cell growth rate and cell cycle were analysed by cell-counting and flow cytometry. Results: Wild-type P53 gene could be quickly and effectively transfected into the cells by adenovirus vector. Wild-type P53 expression could inhibit GLC-82 cell proliferation and induce apoptosis. Conclusion: The results indicated that recombinant adenovirus expressing wild-type P53 might be useful vector for gene therapy of human lung adenocarcinoma.

Key words

Adenovirus vector P53 gene GLC-82 cell Gene therapy 

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Copyright information

© Chinese Journal Of Cancer Research 1998

Authors and Affiliations

  • Li Jian 
    • 1
  • Xia Yongjing 
    • 1
  • Jiang Lei 
    • 1
  • Li Hongxia 
    • 1
  • Hu Yajun 
    • 1
  • Yi Lin 
    • 1
  • Hu Shixue 
    • 2
  • Xu Hongji 
    • 2
  1. 1.Beijing Institute of GeriatricsBeijing HospitalBeijingChina
  2. 2.Department of Molecular OncologyThe University of Texas MD Anderson Cancer CenterHoustonUSA

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