Advertisement

Sugar Tech

, Volume 5, Issue 3, pp 189–191 | Cite as

Effect of methyl jasmonate on sugarcane seedlings

  • G. Seema
  • Manoj K. Srivastava
  • Sangeeta Srivastava
  • Ashok K. Shrivastava
Short Communication

Abstract

Sugarcane seedlings derived from single bud setts of CoLk 8102 were exposed to methyl jasmonate (1,2.5 and 5 ppm). Enzyme activities of catalase, peroxidase, Superoxide dismutase, nitrate reductase, isoenzyme analysis of Superoxide dismutase, proline and soluble protein content were determined after one month. The activities of catalase, Superoxide dismutase, nitrate reductase were lower at 1 ppm compared to control but at 5 ppm these were comparable to the control. The activity of peroxidase was higher than control under all MeJA treatments. The treatment with 2.5 and 5 ppm MeJA increased the number of isoenzymes of Superoxide dismutase in a dose dependent manner while 1 ppm MeJA treated ones had lesser number of isoenzymes as compared to control. Proline content was lower at 1 ppm but increased at 5 ppm. The reduction in enzymes activities at 1 ppm MeJA and increase at 5 ppm MeJA suggest methyl jasmonate regulated expression of these enzymes in sugarcane. These results suggest existence of the methyl jasmonate signalling pathway in sugarcane.

Keywords

Enzyme activities Methyl jasmonate Signalling Sugarcane 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bates, L.S., Waldron, R.P and Teare, I.D. (1973). Rapid determinaton of free proline for water studies.Plant Soil,39: 205–208.CrossRefGoogle Scholar
  2. Beauchamp, C and Fridovich, I. (1971). Superoxide dismutase improved assay and an assay applicable to acrylamide gels.Anal. Biochem.,44: 276–287.PubMedCrossRefGoogle Scholar
  3. Benedetti, C.E., Costa, C.L., Turcinelli, S.R and Arruda, P. (1998). Differential expression of a novel gene in response to coronatine, methyl jasmonate and wounding in the.ciol mutant ofArabidopsis.Plant Physiol.,116: 1037–1042.PubMedCrossRefGoogle Scholar
  4. Bower, N.I., Casu, R.E., Maclean, D.J and Manners, J.M. (2001). Identification of markers of the defence response in sugarcane.Proc. Int. Soc. Sugar Cane Technol.,24: 624–625.Google Scholar
  5. Chen, C.T., Chen, C.M and Kao, C.H. (1994). Methyl jasmonate induces the accumulation of putrescine but not proline in detached rice leaves.J. Plant Physiol.,143: 119–121.Google Scholar
  6. Creelamn, R.A. and Mullet, J.E. (1997). Biosynthesis and action of jasmonates in plants.Ann. Rev. Plant. Physiol. Plant. Mol. Biol,48: 355–381.CrossRefGoogle Scholar
  7. Euler H.Von andJosephson, K. (1927). Uber Katalase I Leibig Ann,452: 158–181.Google Scholar
  8. Jaworski, E.G. (1971). Nitrate reductase assay in intact plant tissue.Biochem. Biophys. Res. Commun.,43: 1272–1279.CrossRefGoogle Scholar
  9. Koetze, D.S andRidgeley, J.R. (1998). Characteristics of a novel cysteine protease expressed in soybean seedlings exposed to methyl jasmonate.URL: http://abstracts.aspb.org/aspb.1998/44/0209.shtml.Google Scholar
  10. Lowry, O.H., Rosenbrough, N.J., Farr, A.L andRandall, R.J. (1951) Protein measurement with Folin Phenol Reagent.J. Biol. Chem.,193: 265–275.PubMedGoogle Scholar
  11. Luck, H. (1963). Peroxidase In: Methods in Enzymatic Analysis (Hu Borgweyer ed) Academic Press, New York 895–897.Google Scholar
  12. Mader, J.C (1999). Effects of Jasmonic Acid, Silver Nitrate and L-AOPP on the distribution of free and conjugated polyamines in roots and shoots ofSolanum tuberosum in vitro.J. Plant Physiol.,154(1): 79–85.Google Scholar
  13. Manchenko, G.P. (1994). Hand book of detection of enzymes on electrophoresis gels. CRC Press Inc. Boca Raton, U.S.A.Google Scholar
  14. Sembdner, G. and Parthier, B. (1993). The biochemistry and the physiological and molecular actions of jasmonates.Ann. Rev. Plant Physiol. Plant Mol. Biol.,44: 569–589.CrossRefGoogle Scholar
  15. Seo, H.S., Song, J.T., Cheong, J.L., Lee, Y.H., Lee, Y.W., Hwang, I, Lee, J.S. and Choi, Y.D. (2001). Jasmonic acid carboxyl methyl transferase: A key enzyme for jasmonate regulated plant responses. PNAS, USA98(8): 4788–4793.CrossRefGoogle Scholar
  16. Watanabe, T. and Sakai, S. (1998). Effects of active oxygen species and methyl jasmonate on expression of the gene for wound inducible 1-aminocyclopropane-carboxylate synthase in winter squash(Cucurbita maxima).Planta,206: 570–576CrossRefGoogle Scholar
  17. Weidhase, R.A.E., Krameil, H.M., Lehmann, J., Liebisch, H.W., Lerbs, W. and Parthier, B. (1987). Methyl jasmonate induced changes in the polypeptide pattern of senescing barley leaf segments.Plant Sci.,51: 177–186.CrossRefGoogle Scholar

Copyright information

© Society for Sugar Research & Promotion 1999

Authors and Affiliations

  • G. Seema
    • 2
  • Manoj K. Srivastava
    • 1
  • Sangeeta Srivastava
    • 2
  • Ashok K. Shrivastava
    • 1
  1. 1.Division of Plant Physiology and BiochemistryIndian Institute of SugarcaneResearchIndia
  2. 2.Division of Crop ImprovementIndian Institute of Sugarcane ResearchLucknow - 226 002, Uttar PradeshIndia

Personalised recommendations