Stms Marker for Tagging High Sugar Genes in Sugarcane
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The use of DNA markers for the genetic analysis and manipulation of desirable agronomic traits has become an increasingly useful tool in sugarcane breeding. DNA was extracted from immature leaves of eight contrasting high and low sugar commercial varieties of sugarcane by c-TAB method, purified and quantified at 20 ng/µl. Parental screening was made by available 46 SSR forward and reverse primers (SMS and STMS) from public domain on agarose and polyacrylamide gel electrophoresis. Bands at 208 and 318 bp were observed by STMS-34 primer (cgtcttgtggattggattgg-forward and tggattgctcaggtgtttca-reverse) in high sugar trait lines but absent in low sugar trait line which may be used as a marker after further validation.
Key wordsSugar gene tagging sugarcane varieties SSR STMS
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