Skip to main content
Log in

The production of xylitol by enzymatic hydrolysis of agricultural wastes

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Agricultural waste products, beech wood and walnut shells, were hydrolyzed at 40°C using mixed crude enzymes produced byPenicillium sp. AHT-1 andRhizomucor pusillus HHT-1.d-xylose, 4.1 g and 15.1 g was produced from the hydrolysis of 100 g of beech wood and walnut shells, respectively. For xylitol production,Candida tropicalis IFO0618 and the waste product hydrolyzed solutions were used. The effects on xylitol production, of adding glucose as a NADPH source,d-xylose and yeast extract, were examined. Finally, a 50% yield of xylitol was obtained by using the beech wood hydrolyzed solution with the addition of 1% yeast extract and 1% glucose at an initial concentration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Rolla, G., A. A. Schele, and S. Assev (1987) Plaque formation and plaque inhibition.Dutsch. Zahnarztl. 42: 39–41.

    Google Scholar 

  2. Van Eys, J., Y. M. Wang, S. Chan, S. Tanphaichitr, and S. M. King (1974) Sugar in nutrition: Xylitol as a therapeutic agent in glucose-6 phosphate dehydrogenase deficiency, 8 ed, pp. 613–631 In: H. L. Sipple and K. W. McNatt (eds.). Academic Press, New York, USA.

    Google Scholar 

  3. Pepper, T. and P. M. Olinger (1988) Xylitol in sugar-free confections.Food Technol. 42: 98–106.

    Google Scholar 

  4. FAO (1999) Food and agriculture organization of the United Nations,FAO production yearbook 1999.

  5. Winkelhausen, E. and S. Kuzmanova (1998) Microbial conversion of D-xylose to xylitol.J. Ferment. Bioeng. 86: 1–14.

    Article  CAS  Google Scholar 

  6. Rahman, A. K. M. S., S. Kawamura, M. Hatsu, M. M. Hoq, and K. Takamizawa (2001) Physicochemical properties of a novel α-L-arabinofuranosidase fromRhizomucor pusillus HHT-1.Can. J. Microbiol. 47: 67–772.

    Google Scholar 

  7. Rahman, A. K. M. S., N. Sugitani, M. Hatsu, and K. Takamizawa (2003) A role of xylanase, α-L-arabinofuranosidase, and xylosidase in xylan degradation.Can. J. Microbiol. 49: 58–64.

    Article  CAS  Google Scholar 

  8. Horitsu, H., Y. Yahashi, K. Takamizawa, K. Kawai, K. Suzuki, and N. Watanabe (1992) Production of xylitol from D-xylose byCandida tropicalis: optimization of production rate.Biotech. Bioeng. 40: 1085–1091.

    Article  CAS  Google Scholar 

  9. Yokoyama, S., T. Suzuki, K. Kawai, H. Horitsu, and K. Takamizawa (1995) Purification, characterization and structure analysis of NADPH dependent D-xylose reductase fromCandida tropicalis.J. Ferment. Bioengi. 79: 217–223.

    Article  CAS  Google Scholar 

  10. Yahashi, Y., H. Horitsu, K. Kawai, T. Suzuki, and K. Takamizawa (1996) Production of xylitol from D-xylose byCandida tropicalis-the effect of D-glucose feeding.J. Ferment. Bioeng. 81: 148–152.

    Article  CAS  Google Scholar 

  11. Bailey, M. J., P. Biely, and K. Poutanen (1992) Interlaboratory testing of methods for assay of xylanase activity.J. Biotechnol. 23: 257–270.

    Article  CAS  Google Scholar 

  12. Cho, C. H., M. Hatsu, and K. Takamizawa (2002) The production of D-xylose by enzymatic hydrolysis of agricultural wastes.Water Sci. Technol. 45: 97–102.

    CAS  Google Scholar 

  13. Oh, D. K. and S. Y. Kim (1998) Increase of xylitol yield by feeding xylose and glucose inCandida tropicalis.Appl. Microbiol Biotechnol. 50: 419–425.

    Article  CAS  Google Scholar 

  14. Kim, Y. S., S. Y. Kim, J. H. Kim, and S. C. Kim (1999) Xylitol production using recombinantSaccharomyces cerevisiae containing multiple xylose reductase genes at chromosomal δ-sequences.J. Biotechnol. 67: 159–171.

    Article  CAS  Google Scholar 

  15. Choi, J.-H., K.-H. Moon, Y.-W. Ryu, and J.-H. Seo (2000) Production of xylitol in cell recycle fermentation ofCandida tropicalis.Biotechnol Lett. 22: 1625–1628.

    Article  CAS  Google Scholar 

  16. Azuma, M. I., J. Keuchi, R. Kinitani, J. Kato, and H. Ooshima (2000) Increase in xylitol production ofCandida tropicalis.Biotechnol. Lett. 22: 1625–1628.

    Article  Google Scholar 

  17. Chung, Y. S., M. D. Kim, W. L. Lee, Y. W. Ryu, J. H. Kim, and J. H. Seo (2002) Stable expression of xylose reductase gene enhance xylitol production in recombinantSaccharomyces cerevisiae.Enzyme Microb. Technol. 20: 809–816.

    Article  Google Scholar 

  18. Kim, M. D., Y. S. Jeun, S. G. Kim, Y. W. Ryu, and J. H. Seo (2002) Comparison of xylitol production in recombinantSaccharomyces cerevisiae strain harboring XYL1 gene ofPichia stipitis and GRE3 gene ofS. cerevisiae.Enzyme Microb. Technol. 31: 862–866.

    Article  CAS  Google Scholar 

  19. Hallborn, J., M. Waldfridsson, U. Airakisinen, H. Ojamo, B. Haln-Hägerdal, M. Penttila, and S. Keränen (1991) Xylitol production by recombinantSaccharomyces cerevisiae Bio/Technology 9: 1090–1095.

    Article  CAS  Google Scholar 

  20. Delgenes, J. P., M. C. Escare, J. M. Laplace, R. Moletta, and J. M. Navarro (1998) Biological production of industrial chemicals,i.e. xylitol and ethanol, from lignocellulose by controlled mixed culture.Ind. Crops Products. 7: 101–111.

    Article  CAS  Google Scholar 

  21. Cho, C. H., S. Aruga, M. Hatsu, T. Suzuki, K. Kawai, and K. Takamizawa (2000) Development of medium by the combined treatment of steam explosion and chemical decomposition of peanut shells for production of xylitol byCandida tropicalis.J. Japan Soc. Waste Manage. Experts 11: 11–20.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kazuhiro Takamizawa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tran, L.H., Yogo, M., Ojima, H. et al. The production of xylitol by enzymatic hydrolysis of agricultural wastes. Biotechnol. Bioprocess Eng. 9, 223–228 (2004). https://doi.org/10.1007/BF02942297

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02942297

Keywords

Navigation