Biotechnology and Bioprocess Engineering

, Volume 6, Issue 1, pp 72–74 | Cite as

Cultivation of transgenicNicotiana tabacum suspension cells in bioreactors for the production of mGM-CSF

  • Sang-Yoon Lee
  • Won Hur
  • Gyu Heon Cho
  • Dong-Il Kim


TransgenicNicotiana tabacum cells were cultivated for the production of murine granulocyte macrophage-colony stimulating factor (mGM-CSF) in both a stirred, tank biore|actor and an airlift bioreactor with draft tube. Cell growth and mGM-CSF production in the airlift bioreactor were found to be better than those achieved in the stirred tank bioreactor. In the airlift bioreactor. 9.0 g/L of cells and 2.2 ng/mL of mGM-CSF were obtained (11.0 g/L and 2.4 ng/mL, respectively in shake flasks). Although the lag period was prolonged and mGM-CSF production was lowered by 33% in the stirred tank bioreactor as compared to the control culture, the maximum cell density was increased up to 12.0 g/L due to better mixing by agitation at the higher cell density.


bioreactor foreign protein mGM-CSF plant cell culture transgenic plant 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2001

Authors and Affiliations

  • Sang-Yoon Lee
    • 1
  • Won Hur
    • 2
  • Gyu Heon Cho
    • 3
  • Dong-Il Kim
    • 1
  1. 1.Department of Biological Engineering and Center of Advanced Bioseparation TechnologyInha UniversityIncheonKorea
  2. 2.Department of BiotechnologyKangwon National UniversityChunchonKorea
  3. 3.Department of Chemical EngineeringKangwon National UniversityChunchonKorea

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