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Immobilization of glucose oxidase and peroxidase and their application in flow-injection analysis for glucose in serum

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Abstract

Glucose oxidase (GOD) and Horseradish peroxidase (HRP) were covalently coupled to alkylamine controlled pore glass by means of glutaraldehyde. About 700–800 U/g of immobilized GOD and 300–400 U/g of immobilized HRP were obtained. Some factors of affecting enzyme immobilization were discussed.

The immobilized enzymes were packed into a plastic tube and used in flow-injection analysis (FIA) for glucose in serum. A good linearity range was observed for this immobilized enzyme system at 20 mg/mL to 1000 mg/dLd-glucose, the recovery was 95.4–103.5%, the within-batch imprecision was 0.8–2.2%, and the between-batch imprecision was 2.2–4.2%. More than 100 samples were measured within an hour. One enzyme column with five units of immobilized GOD and HRP, applied for 50 assays/d, has been used for more than 2 mo.

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References

  1. Johansson, G., Ogren, L., and Olsson, B. (1983),Anal. Chim. Acta 145, 71.

    Article  CAS  Google Scholar 

  2. Hara, T., Toriyama, M., and Imaki, M. (1982),Bull. Chem. Soc. Jpn. 55, 1854.

    Article  CAS  Google Scholar 

  3. Ye, Ying-wu and Mo, Pei-sheng (1986),Updated Clinical Laboratory Methods, Tai An Bronches, Shan Dong Publishing House, China, p. 67 (in Chinese).

    Google Scholar 

  4. Guilbault, G. G. (1976),Handbook of enzymatic methods of analysis, Marcel Dekker, New York, p. 147.

    Google Scholar 

  5. Lowry, O. H. (1951),J. Biolg. Chem. 193, 265.

    CAS  Google Scholar 

  6. Jiang, Cheng-gan (1984),Enzyme Linked Immuno-Sorbance Assay, People’s Health Publishing House, China, p. 22 (in Chinese).

    Google Scholar 

  7. Li, G. X., Wang, Y. X., Kou, X. F., and Zhang, S. Z. (1982),Appl. Biochem. Biotech. 7, 325.

    Article  CAS  Google Scholar 

  8. Ruslling, J. F., Luttrell, G. H., Cullen, L. F., and Papariello, G. J. (1976),Anal. Chem. 48, 1211.

    Article  Google Scholar 

  9. Robinson, P. J., Dunnill, P., and Lilly, M. D. (1971),Biochim. Biophys. Acta 242, 659.

    CAS  Google Scholar 

  10. Waddell, T. G., Leyden, D. E., and DeBello, M. T. (1981),J. Am. Chem. Soc. 103, 5303.

    Article  CAS  Google Scholar 

  11. Jiang, Cheng-gan (1984),Enzyme Linked Immuno-Sorbance Assay, People’s Health Publishing House, China, p. 31 (in Chinese).

    Google Scholar 

Download references

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Authors and Affiliations

  1. National Center for Clinical Laboratory, 100730, Beijing

    Jian Guo & Pei-Sheng Mo

  2. Institute of Microbiology, Academia Sinica, 100080, Beijing, People’s Republic of China

    Gao-Xiang Li

Authors
  1. Jian Guo
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  2. Pei-Sheng Mo
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  3. Gao-Xiang Li
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Guo, J., Mo, PS. & Li, GX. Immobilization of glucose oxidase and peroxidase and their application in flow-injection analysis for glucose in serum. Appl Biochem Biotechnol 23, 15–24 (1990). https://doi.org/10.1007/BF02942049

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  • DOI: https://doi.org/10.1007/BF02942049

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