Abstract
A self-aggregating strain ofSaccharomyces uvarum (U4) was used as a biocatalyst to carry out continuous ethanol fermentation in a tower fermentor equipped with a cell separator. Cell aggregates (2–3 mm) formed a stable packed bed in the fermentor, and the cell separator retained yeast cells effectively. Corn steep liquor was used as a nitrogen source for the fermentation of corn syrup and black strap molasses. An ethanol productivity of 54 g/L/h was reached using corn syrup at a dilution rate of 0.7/h, and sugar concentration in the feed was 15% (w/v). For molasses fermentation, an ethanol productivity of 22 g/L/h was obtained at a dilution rate of 0.7/h, and sugar concentration in the feed was 12.5% (w/v). Ethanol yields obtained from tower fermentation are higher than those obtained from flask fermentation (96% for corn syrup fermentation and 92% for molasses fermentation). No significant loss in fermentation activity was observed after 3 mo of operation.
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References
Del Rosario, E. J. and Pamatong, F. V. (1985),Biotechnol. Lett. 7, 819.
Wada, M., Kato, J., and Chibata, (1981),Appl. Microbiol. Biotechnol. 11, 67.
Navarro, A., Marangoni, H., Plaza, I. M., and Callieri, D. (1984),Biotechnol. Lett. 6, 465.
Linko, Y. Y. and Linko, P. (1981),Biotechnol. Lett. 3, 21.
Arcuri, E. J. (1982),Biotechnol. Bioeng. 24, 595.
Perterson, J. N. and Davidson, B. H. (1991),Appl. Biochem. Biotechnol. 28/29, 685.
Dauglis, A. J., Brown, N. M., Cluett, W. R., and Dunlop, D. B. (1981),Biotechnol. Lett. 3, 651.
Moo-Young, M., Lamptey, J., and Robinson, C. W. (1980),Biotechnol. Lett. 2, 541.
Black, G. M., Webb, C., Matthews, T. M., and Atkinson, B. (1984),Biotechnol. Bioeng. 26, 134.
Douglas, S. I., Michaels, A. S., Robertson, C. R., and Matin, A. (1985),Applied Microbiol. Biotechnol. 23, 85.
Bulock, J. D., Comberbach, D. M., and Ghommich, C. (1984),Chem. Eng. J. 29, B9.
Admassu, W. and Korus, R. (1985),Chem. Eng. J. 31, B1.
Jones, S. T., Korus, R. A., Admassu, W., and Heimsch, R. C. (1984),Biotechnol. Bioeng. 26, 742.
Netto, C. B. and Goma, G. (1987),Biotechnol. Bioeng. 30, 320.
Chen, L. F. and Gong, C. S. (1985),Biotechnol. Bioeng. 14, 257.
Chen, L. F. and Gong, C. S. (1986),Appl. Microbiol. Biotechnol. 24, 25.
Kida, K., Yamadaki, M., Asano, S., Nakata, T., and Snonda, Y. (1989),J. Ferm. Bioeng. 68, 107.
Prince, I. G. and Barford, J. P. (1982),Biotechnol. Lett. 4, 649.
Greenshields, N. R., Yates, J., Sharp, P., and David, T. M. C. (1972),J. Inst. Brew. 79, 236.
Hamamci, H. and Ryu, D. Y. (1988),Appl. Microbiol. Biotechnol. 28, 515.
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Chen, CS., Chan, E., Wang, S.L. et al. Ethanol fermentation in a tower fermenter using self-aggregatingSaccharomyces uvarum . Appl Biochem Biotechnol 45, 531–544 (1994). https://doi.org/10.1007/BF02941828
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DOI: https://doi.org/10.1007/BF02941828