Advertisement

Influence of media on measurement of bacterial populations in the subsurface

Numbers and diversity
  • Anthony V. Palumbo
  • Chuanlun Zhang
  • Shi Liu
  • Shirley P. Scarborough
  • Susan M. Pfiffner
  • Tommy J. Phelps
Session 5 Environmental Biotechnology

Abstract

To examine the factors contributing to microbial heterogeneity, the US Department of Energy Subsurface Science Program has initiated research at a Coastal Plain site near Oyster, VA. Sediments at the site are composed of unconsolidated, fine to coarse beach sands and gravel. The influence of microbiological culture media on the measurement of colony-forming units (CFU) and microbial diversity was examined in this study. Two low-nutrient media formulations (i.e., dilute peptone, tryptone, yeast extract, and glucose medium [PTYG] and a soil extract medium) gave equivalent estimates of CFU (maximum of about 6.5 × 105 CFU/g). However, a higher nutrient medium, organic nutrients with brain-heart infusion (N/BHI), gave lower numbers than the dilute PTYG. A medium used to enumerate fungi also gave significantly lower counts than the dilute PTYG medium. Although the correlations were highly significant among total CFU numbers measured with the different types of media, we found few significant correlations in several indices of microbial diversity, and the correlation coefficients were below 0.25. Thus, CFU was a relatively robust measurement, but microbial diversity was not consistent between the media. Species richness was highest with the higher nutrient N/BHI medium, and N/BHI may be a better choice for a study of diversity as determined by colony morphology.

Index Entries

Diversity CFU bioremediation media, subsurface microbiology fungi 

References

  1. 1.
    Kaneko, T., Atlas, R. M., and Krichevsky, M. (1977),Nature 270, 596–599.CrossRefGoogle Scholar
  2. 2.
    Mills, A. L. and Wassel, R. A. (1980),Appl. Environ. Microbiol. 40, 578–586.Google Scholar
  3. 3.
    Atlas, R. M. (1984), inCurrent Perspectives in Microbial Ecology, Klug, J. and Reddy, C. A., eds., American Society for Microbiology, Washington, DC, pp. 540–545.Google Scholar
  4. 4.
    Atlas, R. M. (1984), inAdvances in Microbial Ecology, vol. 7, Plenum, New York, pp. 1–47.Google Scholar
  5. 5.
    Bone, T. L. and Balkwill, D. L. (1988), Microb. Ecol.16, 49–64.CrossRefGoogle Scholar
  6. 6.
    Balkwill, D. L., Fredrickson, J. K., and Thomas, J. M. (1989),Appl. Environ. Microbiol. 55, 1058–1065.Google Scholar
  7. 7.
    Fredrickson, J. K., Balkwill, D. L., Zachara, J. M., Li, S. W., Brockman, F. J., and Simmons, M. A. (1991),Appl. Environ. Microbiol. 57, 402–411.Google Scholar
  8. 8.
    Balkwill, D. L. and Ghiorse, W. C. (1985),Appl. Environ. Microbiol. 50, 580–588.Google Scholar
  9. 9.
    Brockman, F. J., Kieft, T. L., Fredrickson, J. K., Bjornstad, B. N., Li, S.-M. W., Spangenburg, W., and Long, P. E. (1992),Microb. Ecol. 23, 279–301.CrossRefGoogle Scholar
  10. 10.
    Difco (1984),Difco Manual: Dehydrated Culture Media and Reagents for Microbiology, Difco Laboratories, Detroit.Google Scholar
  11. 11.
    Balkwill, D. (1989),Geomicrobiol. J. 7, 33–52.CrossRefGoogle Scholar
  12. 12.
    Mixon, R. B. (1985), US Geol. Surv. Prof. Paper. 1067-C, pp. 53.Google Scholar
  13. 13.
    Russell, B., Phelps, T. J., Griffin, T., and Sargent, K. L. (1992),Water Well Winter 92, 96–104.Google Scholar
  14. 14.
    Smibert, R. M. and Krieg, N. R. (1994),Methods for General and Molecular Bacteriology, Gerhardt, P., Murry, R. G. E., Wood, W. A., and Krieg, N. R., eds., American Society for Microbiology, Washington, DC, pp. 607–654.Google Scholar
  15. 15.
    Odum, E. P. (1971),Fundamentals of Ecology. W. B. Saunders Co., Philadelphia.Google Scholar
  16. 16.
    Palumbo, A. V., Zhang, C., Phelps, T. J., Pfiffner, S. M., Ringleberg, D., and Chapatwala. K.Microb. Ecol. Submitted for publication.Google Scholar
  17. 17.
    Chapatwala, K. D., Babu, G. V., Armstead, E., Palumbo, A. V., Zhang, C., and Phelps, T.,Appl. Biochem. Biotechnol. 57/58, 813–821.Google Scholar
  18. 18.
    Bianchi, M. A. G. and Bianchi, A. J. M. (1982),Microb. Ecol. 8, 61–69.CrossRefGoogle Scholar
  19. 19.
    Olsen, K. D. and Sizemore, R. K. (1981),Dev. Ind. Microbiol. 22, 685–694.Google Scholar
  20. 20.
    Troussellier, M. and Legendre, P. (1981),Microb. Ecol. 7, 283–296.CrossRefGoogle Scholar
  21. 21.
    Hauxhurst, J. D., Kaneko, T., and Atlas, R. M. (1981),Microbial. Ecol. 7, 167–182.CrossRefGoogle Scholar
  22. 22.
    Stahl D. A., Lane, D. J., Olsen, G. C., and Pace, N. R. (1984),Science 224, 409–411.CrossRefGoogle Scholar
  23. 23.
    Stahl D. A., Lane, D. J., Olsen, G. C., and Pace, N. R. (1985),Appl. Environ. Microbiol. 49, 1379–1384.Google Scholar
  24. 24.
    Muyzer, G., De Wall, E. C., and Uitterlinden, A. G. (1993),Appl. Environ. Microbiol. 59, 695–700.Google Scholar
  25. 25.
    Fuhrman, J. A., McCallum, K., and Davis, A. A. (1993),Appl. Environ. Microbiol. 59, 1294–1302.Google Scholar
  26. 26.
    Moyer, C. L., Dobbs, F. C., and Karl, D. M. (1994),Appl. Environ. Microbiol. 60, 871–879.Google Scholar
  27. 27.
    Haldeman, D. L. and Amy, P. S. (1993),Appl. Environ. Microbiol. 59, 933–935.Google Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Anthony V. Palumbo
    • 1
  • Chuanlun Zhang
    • 1
  • Shi Liu
    • 1
  • Shirley P. Scarborough
    • 1
  • Susan M. Pfiffner
    • 1
  • Tommy J. Phelps
    • 1
  1. 1.Environmental Sciences DivisionOak Ridge National LaboratoryOak Ridge

Personalised recommendations