Production ofl-malic acid from fumaric acid by resting cells ofBrevibacterium sp.
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The ability of the resting cells ofBrevibacterium sp. B2D, a flocculent strain, to convert fumaric acid tol-malic acid was investigated under different fermentation conditions. The optimal temperature for the bioconversion is approx 36°C, and the optimal pH is around 6.4. The bacteria do not require special cultural conditions for the development of fumarase. In the presence of relatively high concentrations of fumarate (60 g/L), the bacterial cells exhibited a high rate ofl-malic acid production with high yield without the coproduction of unwanted byproducts like succinic acid. A yield of 89.8% ofl-malic acid from fumaric acid and an average specific productivity of 0.41 g/g/h was obtained. The presence of surfactant, even at low concentrations like 0.02%, resulted in over a twofold increase in specific malic acid production rate.