Advertisement

Production ofl-malic acid from fumaric acid by resting cells ofBrevibacterium sp.

  • C. S. Gong
  • Ningjun Cao
  • Yan Sun
  • G. T. Tsao
Session 3 Bioprocessing Research

Abstract

The ability of the resting cells ofBrevibacterium sp. B2D, a flocculent strain, to convert fumaric acid tol-malic acid was investigated under different fermentation conditions. The optimal temperature for the bioconversion is approx 36°C, and the optimal pH is around 6.4. The bacteria do not require special cultural conditions for the development of fumarase. In the presence of relatively high concentrations of fumarate (60 g/L), the bacterial cells exhibited a high rate ofl-malic acid production with high yield without the coproduction of unwanted byproducts like succinic acid. A yield of 89.8% ofl-malic acid from fumaric acid and an average specific productivity of 0.41 g/g/h was obtained. The presence of surfactant, even at low concentrations like 0.02%, resulted in over a twofold increase in specific malic acid production rate.

Index Entries

Fumaric acid l-malic acid Brevibacterium sp. B2D fumarase effect of surfactant 

References

  1. 1.
    Buck, R. E. and Mottern, H. H. (1947),Ind. Eng. Chem. 39, 1087–1089.CrossRefGoogle Scholar
  2. 2.
    Kamenetsky, M., Kharas, G. B., Watson, K., Caywood, G. A., and Kitchell, J. P. (1993),Poly. Materials 69, 333–337.Google Scholar
  3. 3.
    Bercovitz, A., Peleg, Y., Battat, E., Rokem, J. S., and Goldberg, I. (1990),Appl. Environ. Microbiol. 56, 1594–1597.Google Scholar
  4. 4.
    Battat, E., Peleg, Y., Bercovitz, A., Rokem, J. S., and Goldberg, I. (1991),Biotechnol. Bioeng. 37, 1108–1116.CrossRefGoogle Scholar
  5. 5.
    Yamamoto, K., Tosa, T., Yamashita, K., and Chibata, I. (1976),Eur. J. Appl. Microbiol. 3, 169–183.CrossRefGoogle Scholar
  6. 6.
    Takata, I., Yamamoto, K., Tosa, T., and Chibata, I. (1980),Enzyme Microb. Technol. 2, 30–36.CrossRefGoogle Scholar
  7. 7.
    Peleg, Y., Stieglitz, B., and Goldberg, I. (1988),Appl. Microbiol. Biotechnol. 28, 69–75.CrossRefGoogle Scholar
  8. 8.
    Figueiredo, Z. M. B. and Carvalho, L. B., Jr. (1991),Appl. Biochem. Biotechnol. 30, 217–224.CrossRefGoogle Scholar
  9. 9.
    Neufeld, R. J., Peleg, Y., Rokem, J. S., Pines, O., and Goldberg, I. (1991),Enzyme Microb. Technol. 13, 991–996.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • C. S. Gong
    • 1
  • Ningjun Cao
    • 2
  • Yan Sun
    • 3
  • G. T. Tsao
    • 1
  1. 1.Laboratory of Renewable Resources EngineeringPurdue UniversityWest Lafayette
  2. 2.Food Science DepartmentPurdue UniversityWest Lafayette
  3. 3.The Store Institute of Grain Storage TechnologyBeijingChina

Personalised recommendations