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Production ofl-malic acid from fumaric acid by resting cells ofBrevibacterium sp.

  • Session 3 Bioprocessing Research
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Abstract

The ability of the resting cells ofBrevibacterium sp. B2D, a flocculent strain, to convert fumaric acid tol-malic acid was investigated under different fermentation conditions. The optimal temperature for the bioconversion is approx 36°C, and the optimal pH is around 6.4. The bacteria do not require special cultural conditions for the development of fumarase. In the presence of relatively high concentrations of fumarate (60 g/L), the bacterial cells exhibited a high rate ofl-malic acid production with high yield without the coproduction of unwanted byproducts like succinic acid. A yield of 89.8% ofl-malic acid from fumaric acid and an average specific productivity of 0.41 g/g/h was obtained. The presence of surfactant, even at low concentrations like 0.02%, resulted in over a twofold increase in specific malic acid production rate.

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Authors and Affiliations

  1. Laboratory of Renewable Resources Engineering, Purdue University, 47907, West Lafayette, IN

    C. S. Gong & G. T. Tsao

  2. Food Science Department, Purdue University, 47907, West Lafayette, IN

    Ningjun Cao

  3. The Store Institute of Grain Storage Technology, Beijing, China

    Yan Sun

Authors
  1. C. S. Gong
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  2. Ningjun Cao
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  3. Yan Sun
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  4. G. T. Tsao
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Gong, C.S., Cao, N., Sun, Y. et al. Production ofl-malic acid from fumaric acid by resting cells ofBrevibacterium sp. . Appl Biochem Biotechnol 57, 481–487 (1996). https://doi.org/10.1007/BF02941728

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  • DOI: https://doi.org/10.1007/BF02941728

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