Production ofl-malic acid from fumaric acid by resting cells ofBrevibacterium sp.

  • C. S. Gong
  • Ningjun Cao
  • Yan Sun
  • G. T. Tsao
Session 3 Bioprocessing Research


The ability of the resting cells ofBrevibacterium sp. B2D, a flocculent strain, to convert fumaric acid tol-malic acid was investigated under different fermentation conditions. The optimal temperature for the bioconversion is approx 36°C, and the optimal pH is around 6.4. The bacteria do not require special cultural conditions for the development of fumarase. In the presence of relatively high concentrations of fumarate (60 g/L), the bacterial cells exhibited a high rate ofl-malic acid production with high yield without the coproduction of unwanted byproducts like succinic acid. A yield of 89.8% ofl-malic acid from fumaric acid and an average specific productivity of 0.41 g/g/h was obtained. The presence of surfactant, even at low concentrations like 0.02%, resulted in over a twofold increase in specific malic acid production rate.

Index Entries

Fumaric acid l-malic acid Brevibacterium sp. B2D fumarase effect of surfactant 


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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • C. S. Gong
    • 1
  • Ningjun Cao
    • 2
  • Yan Sun
    • 3
  • G. T. Tsao
    • 1
  1. 1.Laboratory of Renewable Resources EngineeringPurdue UniversityWest Lafayette
  2. 2.Food Science DepartmentPurdue UniversityWest Lafayette
  3. 3.The Store Institute of Grain Storage TechnologyBeijingChina

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