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Secretion of active urokinase-type plasminogen activator from the yeastYarrowia lipolytica

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Abstract

In order to study the secretion of the human urokinase-type plasminogen activator, u-PA, from the yeastYarrowia lipolytica, three kinds of integrative expression vector were constructed. These vectors differed only in their secretion control regions, pre-, pre-dip- (dipeptide stretch) or pre-dip-pro sequences of the alkaline extracellular protease, which were joined inframe to the human u-PA cDNA. The recombinantY. lipolytica strains, transformed with the expression vectors, secreted the hyperglycosylated u-PA. A fibrin plate assay of the culture supernatants showed that the hyperglycosylated u-PA proteins could catalyze fibrinolysis, and that the pre-dip sequence was the most efficient secretory signal for the secretion of the u-PA fromY. lipolytica. This result suggests thatY. lipolytica can be developed as a potential host for the production of recombinant human u-PA.

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Authors and Affiliations

  1. Department of Microbiology, Chungnam National University, 305-764, Daejeon, Korea

    Ho Myoung Ryu, Woo Kyu Kang & Jeong-Yoon Kim

  2. Korea Research Institute of Bioscience and Biotechnology, 305-764, Daejeon, Korea

    Hyun Ah Kang

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  1. Ho Myoung Ryu
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  2. Woo Kyu Kang
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  4. Jeong-Yoon Kim
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Correspondence to Jeong-Yoon Kim.

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Ryu, H.M., Kang, W.K., Kang, H.A. et al. Secretion of active urokinase-type plasminogen activator from the yeastYarrowia lipolytica . Biotechnol Bioproc E 8, 162–165 (2003). https://doi.org/10.1007/BF02940274

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  • DOI: https://doi.org/10.1007/BF02940274

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