Advertisement

Mycotoxin Research

, Volume 19, Issue 1, pp 69–72 | Cite as

Development and applications of a yeast-based bioassay for the mycotoxin zearalenone

  • R. Mitterbauer
  • H. Bachmann
  • B. Poppenberger
  • N. Safaie
  • G. Adam
Article

Abstract

Zearalenone (ZON) is a non-steroidal estrogenic mycotoxin produced by plant pathogenic species ofFusarium. As a consequence of infection withF. culmorum andF. graminearum, ZON can be found in cereals and derived food products. Several countries have established monitoring programs and guidelines for ZON levels in grain intended for human consumption and animal feed. We have developed a sensitive yeast bioassay allowing detection of the estrogenic activity of ZON in cereal extracts without requiring further clean up steps. The high sensitivity makes this assay suitable for low cost monitoring of contamination of small grain cereals with estrogenicFusarium mycotoxins, but also attractive as a tool for basic research. We have successfully used yeast indicator strains to screen for mutants ofF. graminearum which no longer produce detectable amounts of ZON, and have identified a plant cDNA encoding a ZON detoxification enzyme.

Keywords

zearalenone estrogenic bioassay Fusarium 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kuiper-Goodman T, Scott PM, Watanabe H (1987) Risk assessment of the mycotoxin zearalenone. Regul. Toxicol. Pharmacol. 7:253–306PubMedCrossRefGoogle Scholar
  2. 2.
    Mirocha CJ, and Christensen CM (1974) Estrogenic mycotoxins synthesized by Fusarium. p. 129–148. In I.F.H. Purchase (ed.), Mycotoxins, Elsevier, AmsterdamGoogle Scholar
  3. 3.
    Bundesministerium für Gesundheit, Sport und Konsumentenschutz (1993) Richtwerte für Mykotoxine in Getreide für den menschlichen Genuß. (GZ 32.110/1-III/B/1b/93). Mitteilungen der österreichischen Sanitätsverwaltung 94(7–8):360Google Scholar
  4. 4.
    Lew H (1999) Empfohlenes Richtwerteschema für Desoxynivalenol (Vomitoxin) und Zearalenon in Futter. Der Förderungsdienst 47(5):157Google Scholar
  5. 5.
    Bundesministerium für Ernährung, Landwirtschaft und Forsten (2000) Orientierungswerte für die Beurteilung der Gehalte an Deoxynivalenol und Zearalenon in Futtermitteln im Rahmen des §3 des Futtermittelgesetzes im Futter von Schwein, Rind und Huhn. BML Rdschr. 2000-324-3830/323Google Scholar
  6. 6.
    Pierrat B, Heery DM, Lemoine Y, and Losson R (1992) Functional analysis of the human estrogen receptor using a phenotypic transactivation assay in yeast. Gene 119: 237–245PubMedCrossRefGoogle Scholar

Copyright information

© Society of Mycotoxin Research and Springer 2003

Authors and Affiliations

  • R. Mitterbauer
    • 1
  • H. Bachmann
    • 1
  • B. Poppenberger
    • 1
  • N. Safaie
    • 2
  • G. Adam
    • 1
  1. 1.Center of Applied GeneticsBOKU — University of Natural Resources and Applied Life SciencesViennaAustria
  2. 2.College of AgricultureTarbiat Modarres UniversityTehranIslamic Republic of Iran

Personalised recommendations