Abstract
Uptake of radiolabelled ochratoxin A (OTA) into porcine urinary bladder epithelial cells (PUBEC) was measured at neutral (pH 7.5) or acidic (pH 5.0) conditions. Genotoxicity of OTA was evaluated with the Comet assay and cytotoxicity with the neutral red uptake assay.
At acidic pH-conditions, the bladder cells were able to take up more OTA than at neutral conditions. Cytotoxic effects were not increased at pH 5.0 compared to pH 7.5, but higher OTA uptake correlated with stronger genotoxic effects in the Comet assay at pH 5.0 compared to pH 7.5.
These results demonstrate that uptake of OTA has to be regarded as an important factor for the toxicity of OTA as adverse effects depend on the amount of OTA taken up by the cells.
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References
Babich H, Borenfreund E (1992) Neutral red assay for toxicology in vitro. In: Watson RR (ed.) In Vitro Methods of Toxicology, chapter 17, CRC Press, Boca Raton, Ann Arbor, London, Tokyo, pp 237–251
Föllmann W, Guhe C, Mähler S, and Bolt HM (2000) Cultured porcine urinary bladder epithelial cells (PUBEC) as a screening model for genotoxic effects of aromatic amines: Characterization and application of the cell culture model. ATLA 28: 833–854; 2000
Föllmann, W, Lebrun S, Kullik B, Koch M, Römer HC, and Golka K (2000) Cytotoxicity of ochratoxin A and citrinin in different cell types in vitro. Mycotoxin Res. 16: 123–127
Lebrun S, and Föllmann W (2002) Detection of Ochratoxin A-induced DNA-damage in MDCK cells by alkaline single cell gel electrophoresis (Comet-Assay). Arch. Toxicol. 75: 734–741
Schwerdt G, Freudinger R, Silbernagl S, and Gekle M (1998) Apical uptake of radiolabelled ochratoxin A into Madin-Darby canine kidney cells. Toxicology 131: 193–202
Singh NP, McCoy MT, Tice RR, and Schneider EL (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 175: 184–191
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Föllmann, W., Lebrun, S. Uptake and genotoxic effects of ochratoxin A in cultured porcine urinary bladder epithelial cells. Mycotox Res 19, 24–26 (2003). https://doi.org/10.1007/BF02940086
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DOI: https://doi.org/10.1007/BF02940086