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Mycotoxin Research

, Volume 16, Supplement 2, pp 224–226 | Cite as

Analysis of aflatoxins in various food and feed matrices-results of international validation studies

  • Stroka J 
  • Anklam E 
Article

Abstract

Collaborative studies were conducted to evaluate the effectiveness of analytical methods based on immunoaffinity column clean-up prior to high performance liquid chromatography (HPLC) for the determination of aflatoxin B1 and total aflatoxins at European regulatory limits in various food products (4 ng/g total aflatoxins, 2 ng/g aflatoxin B1), baby food (0.1 ng/g aflatoxin B1 in discussion) and feeding stuff (1 ng/g aflatoxin B1 in discussion).

The methods showed acceptable within-laboratory and between-laboratory precision for all matrices at these low levels for the determination of both, total aflatoxins and aflatoxin B1.

Keywords

High Performance Liquid Chromatography Aflatoxin Peanut Butter Baby Food Immunoaffinity Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Gemeinsame Arbeiten konnten durchgeführt werden, um die Wirksamkeit analytischer Methoden auf der Basis von Affinitätssäulen zu evaluieren. Hier wurde vor allem die Hochdruckflüssigkeitschromatographie (HPLC) zur Bestimmung von Aflatoxin B1 und Gesamtaflatoxinen nach den europäischen Regelwerten (Nahrungsmittel: 4 ng/g Gesamtaflatoxine und 2 ng/g Aflatoxin B1, Babynahrung: 0,1 ng/g Aflatoxin B1 als Richtwert in der Diskussion, Futtermittel: 1 ng/g Aflatoxin B1 in der Diskussion) für verschiedene Produkte überprüft. Die Methoden mit allen angewendeten Matrices lieferten akzeptierbare Ergebnisse sowohl bei Vergleichen innerhalb eines Labors als auch bei solchen aus verschiedenen Laboratorien.

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References

  1. 1.
    Commission Regulation (98/53/EC) of 16. July 1998, Laying down the sampling methods and the methods of analysis for the official control of the levels for certain contaminants in foodstuffs. Official Journal of the European Communities, L201/93.Google Scholar
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    Horwitz W, Albert, R, (1991) JAOAC Int 74: 718–744.Google Scholar
  3. 3.
    Stroka J, Anklam E, Jörissen U, Gilbert J (2000) Immunoaffinity column cleanup with liquid chromatography using post-column bromination for determination of aflatoxins in peanut butter, pistachio paste, fig paste, and paprika powder: collaborative study. JAOAC Int 83: 320–340Google Scholar
  4. 4.
    Stroka J, Anklam E, Jörissen U, Gilbert J (2000) Immunoaffinity column clean-up with liquid chromatography using post-column bromination for determination of aflatoxin B1 in baby food (infant formula): collaborative study. JAOAC Int in printGoogle Scholar
  5. 5.
    Stroka J, Reutter M, von Holst C, Anklam E (2000) Immunoaffinity column clean-up with liquid chromatography using post-column bromination for the determination of aflatoxin B1 in animal feeding stuff: collaborative study. J AOAC Int submitted for publicationGoogle Scholar

Copyright information

© Society of Mycotoxin Research and Springer 2000

Authors and Affiliations

  • Stroka J 
    • 1
  • Anklam E 
    • 1
  1. 1.Institute for Health and Consumer Protection, Joint Research Centre of the European CommunitiesFood Products and Consumer Goods UnitIspra (VA)Italy

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