Journal of Nuclear Cardiology

, Volume 1, Issue 4, pp 351–364 | Cite as

Myocardial uptake of thallium 201 and technetium 99m-labeled sestamibi after ischemia and reperfusion: Comparison by quantitative dual-tracer autoradiography in rabbits

  • Howard Weinstein
  • Christopher P. Reinhardt
  • John F. Wironen
  • Jeffrey A. Leppo
Original Articles



Myocardial scintigraphy with99mTc-labeled sestamibi (99mTc-sestamibi) or201Tl is used to assess regional perfusion in acute coronary syndromes associated with metabolic or functional abnormalities, such as acute coronary thrombosis with reperfusion and ischemia at rest. However, the initial uptake of these agents may be affected by a recent ischemic insult because the myocardial retention of these tracers depends on cellular metabolism.

Methods and Results

Accordingly,99mTc-sestamibi and201Tl were injected simultaneously in rabbits after transient brief (10 to 15 minutes, group I) or prolonged (45 to 60 minutes, group II) coronary occlusion. Accumulated subendocardial and subepicardial99mTc-sestamibi and corresponding201Tl activity were determined from autoradiographs of 30 µm short-axis slices comounted with serial tissue standards. Circumferential99mTc-sestamibi and201Tl activity profiles closely overlapped in both groups. The initial global and segmental myocardial activity per unit blood flow within the ischemic zone did not differ from unity for either tracer regardless of the duration of the ischemic insult. The initial myocardial uptake of both99mTc-sestamibi and201Tl after an acute ischemic insult reflected predominantly coronary blood flow, independent of myocardial viability.


Thus this study supports the use of both99mTc-sestamibi and201Tl as perfusion probes in acute coronary syndromes characterized by acute occlusion and reperfusion.

Key Words

sestamibi thallium 201 myocardial viability 


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Copyright information

© American Society of Nuclear Cardiology 1994

Authors and Affiliations

  • Howard Weinstein
    • 1
  • Christopher P. Reinhardt
    • 1
  • John F. Wironen
    • 1
  • Jeffrey A. Leppo
    • 1
  1. 1.From the Myocardial Isotope Research Laboratory, Departments of Nuclear Medicine and Medicine (Division of Cardiology)University of Massachusetts Medical CenterWorcester

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