Salivary SIgA and SIgA 1 in coeliac disease, inflammatory bowel disease and controls
Levels of secretory IgA1 (SIgA1) in the saliva have not been measured previously in either coeliac disease (CD) or inflammatory bowel disease (IBD). Saliva was collected from coeliacs, IBD patients and controls. The concentration of total SIgA in saliva was measured by enzyme linked immunosorbent assay (ELISA) with an anti-human SIgA antibody as the bound phase and human SIgA isolated from colostrum as the standard. The concentration of SIgA1 was determined using an ELISA with a lectin with a high affinity for human SIgA1. The IBD patients have a significantly higher concentration of SIgA1 than the controls. The rate of secretion of saliva and %SIgA1 was significantly lower in coeliacs than in the control and IBD groups. The rate of secretion of SIgA1 was significantly higher in the IBD than in the coeliacs. We describe hitherto unreported levels of SIgA1 in CD and IBD.
KeywordsInflammatory Bowel Disease Celiac Disease Inflammatory Bowel Disease Patient Saliva Sample Coeliac Disease
Unable to display preview. Download preview PDF.
- 1.Hanson, L. A. Comparative immunological studies of the immunoglobulins of human milk and blood serum. Int. Arch. Appl. Immunol. 1961; 18: 241–267.Google Scholar
- 5.Mearin, F., Mearin, M. L., Pena, A. S. Distribution of IgA1 and IgA2 immunocytes in the jejunum of adult coeliac patients and controls. J. Clin. Nutr. Gastroenterol. 1986; 1: 79–82.Google Scholar
- 6.Baklein, K., Brandzaeg, P., Fausa, O. Immunoglobulin in jejunal mucosa and serum from patients with adult coeliac disease. Scand. J. Gastroent. 1977; 12: 149–159.Google Scholar
- 10.MacDermott, R. P., Nash, G. S., Bertovich, M. J., Seiden, M. V., Bragdon, M. J., Bealf, M. G. Alterations of IgM, IgG and IgA synthesis and secretions by peripheral blood and intestinal mononuclear cells from patients with ulcerative colitis and Crohn’s disease. Gastroenterology 1981; 81: 844–852.PubMedGoogle Scholar
- 11.Brandtzeag, P., Halstensen, T. S., Kett, K., Krajci, P., Kvale, D., Rognum, T. O., Scott, H., Sollid, L. M. Immunobiology and immunopathology of the human gut mucosa: Humoral immunity and intraepithelial lymphocytes. Gastroenterology 1989; 97: 1562–1584.Google Scholar
- 14.Gibney, M. J., Brady, C. Systemic and salivary antibodies to dietary antigens (gliadin, casein and ovalbumin) in treated and untreated coeliac disease and in Crohn’s disease. Eur. J. Intern. Med. 1991; 2: 115–119.Google Scholar
- 20.Clarke, J. A., Callicoat, P. A., Brenner, N. A., Bradley, C. A., Smith, D. M. Selective IgA deficiency in blood donors. Am. J. Clin. Pathol. 1983; 80: 210–213.Google Scholar