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Vibrio Cholerae neuraminidase susceptible n-acetyl neuraminic acid (sialic acid) levels in human leukaemia

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Summary

Cell surface vibrio Cholerae neuraminidase (VCN) susceptible sialic acid has been studied in leucocytes of normal human subjects and patients with acute myeloid, acute lymphatic and chronic lymphatic leukaemia as well as in myeloid metaplasia. The mean VCN susceptible sialic acid released in normal lymphocytes was 222±72 n mole/109 cells as compared to 158±n mole/109 cells in leukaemic lymphocytes, and 178±48 n mole/109 cells in leukaemic granulocytes. This release of surface sialic acid by VCN may play a part in increasing immunogenicity of leukaemic cells for immunotherapy.

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References

  • Aminoff, P. 1961. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem. J. 81, 384.

    PubMed  CAS  Google Scholar 

  • Bekesi, J. G., St. Arneault, G. and Holland, J. F. 1971. Increase in leukaemia L1210 immunogenicity by Vibrio Cholerae neuraminidase treatment. Cancer Res. 31, 2130.

    PubMed  CAS  Google Scholar 

  • Bekesi, J. G., St. Arneault, G., Walter, L. and Holland, J. F. 1972. Immunogenicity of leukaemia L1210 cells after neuraminidase treatment. J. Natl. Cancer Inst. 49, 107.

    PubMed  CAS  Google Scholar 

  • Gottschalk, A. and Bhargava, A. S. 1971. The Enzymes, Vol. V, 3rd Ed. New York, Academic Press, p. 321.

    Google Scholar 

  • Harris, R., Ukaeljiofo, E., Pierce, G. E. and Yang, J. P. 1969. Cellular and humoral immunity to different types of human neoplasms. Nature (Lond.) 220, 1352.

    Google Scholar 

  • Hogan Ryan, M. A. and Fennelly, J. J. 1978. Neuraminidase-like effect of vitamin A on cell surface. Eur. J. Cancer. 14, 113.

    PubMed  CAS  Google Scholar 

  • Im, H. M. and Simmons, R. L. 1971. Modification of graft versus host disease by neuraminidase treatment of donor cells. Transplantation, 12, 472.

    Article  PubMed  CAS  Google Scholar 

  • Kornfeld, S. 1969. Decreased phytohaemagglutinin receptor sites in chronic lymphocytic leukaemia. Biochem. Biophys. Acta, 192, 542.

    PubMed  CAS  Google Scholar 

  • Lichtmann, M. A. and Weed, R. I. 1970. Electrophoretic mobility and N-acetylneuraminic acid content of normal and leukaemic lymphocytes and granulocytes. Blood, 35, 12.

    Google Scholar 

  • Lindenmann, J. and Klein, P. A. 1967. Immunological aspects of viral oncolysis: recent results. Cancer Res. 9, 1.

    Google Scholar 

  • Lundgren, G. and Simmons, R. L. 1971. Effect of neuraminidase on the stimulatory capacity of cells in mixed lymphocyte cultures. Clin Exptl. Immunol. 9, 915.

    CAS  Google Scholar 

  • Mehrishi, J. N. 1974. Cell surface membrane interactions with special reference to lymphocytes in leukaemia. Behring inst. Mitt No. 55, 290.

    CAS  Google Scholar 

  • Mehrishi, J. N. and Thompson, A. E. R. 1968. Relationship between pH and electrophoretic mobility for lymphocytes circulating in chronic lymphatic leukaemia. Nature (Lond), 219, 1080.

    Article  CAS  Google Scholar 

  • McClelland, D. A. and Bridges, J. M. 1973. The total N-Acetyl neuraminic acid content of normal and leukaemic lymphocytes. Br. J. Cancer. 27, 114.

    PubMed  CAS  Google Scholar 

  • Powles, R. L., Crowther, D., Bateman, C. J. L., Beard, M. E. J., McElwain, T. J., Russell, J., Lister, T. A., Whitehouse, J. M., Wrigley, P. F. M., Pike, M., Alexander, P., Hamilton-Fairley, G. 1973. Immunotherapy for acute myelogenous leukaemia. Br. J. Cancer 28, 365.

    PubMed  CAS  Google Scholar 

  • Rios, A. and Simmons, R. L. 1972. Comparative effect of Mycobacterium Bovis and neuraminidase treated tumour cells on the growth of established methylcholanthrene fibrosarcomas in syngeneic mice. Cancer Research 32, 16.

    PubMed  CAS  Google Scholar 

  • Rundell, J. O., Sato, T., Wetzelberger, E., Veda, H., Seiler, F. R., Sedlacek, H. H. 1974. Comparative studies on properties of lysosomes in L1210 leukaemia cells underin vivo andin vitro conditions. Lab. Invest. 30, 279.

    PubMed  CAS  Google Scholar 

  • Seiler, F. R. and Sedlack, H. H. 1974. Alterations of immunological phenomena by neuraminidase. Behring, Inst. Mitt., No. 55, 254

    Google Scholar 

  • Sethi, K. K. and Brandis, H. 1973. Synergistic cytoxic effect of macrophages and normal mouse serum on neuraminidase-treated leukaemia cells. Europ. J. Cancer 9, 809

    CAS  Google Scholar 

  • Simmons, R. L., Rios, A. 1974. Immunospecific regression of methycholanthrene fibrosarcoma with the use of neuraminidase. Israel Med. Sci. 10, 925.

    Google Scholar 

  • Warren, L. 1959. The thiorbarbituric acid assay of sialic acids. J. Biol. Chem. 234, 1971.

    PubMed  CAS  Google Scholar 

  • Weiss, L., Mayhew, E., Ulrich, K. 1966. The effects of neuraminidase on the phagocytic process in human monocytes. Lab. Invest. 15, 1304.

    PubMed  CAS  Google Scholar 

  • Weiss, L. 1965. Studies on cell deformability. I. Effect of surface charge. J Cell Biol. 26, 735.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine and Therapeutics, University College and St. Vincent's Hospital, Dublin 4

    Antoinette Hogan Ryan & J. J. Fennelly

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  1. Antoinette Hogan Ryan
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  2. J. J. Fennelly
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Ryan, A.H., Fennelly, J.J. Vibrio Cholerae neuraminidase susceptible n-acetyl neuraminic acid (sialic acid) levels in human leukaemia. I.J.M.S. 147, 308–312 (1978). https://doi.org/10.1007/BF02939424

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  • DOI: https://doi.org/10.1007/BF02939424

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