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Ricerca in clinica e in laboratorio

, Volume 10, Issue 3, pp 511–519 | Cite as

Immunoreactive trypsin on dried-blood spots as a possible neonatal test for cystic fibrosis

I. Evaluation of the method and preliminary field trial
  • Roberto Dominici
  • Fabrizio Monaco
  • Silvana Morano
  • Italo Antonozzi
Original Contributions

Summary

A method for blood spot immunoreactive trypsin (IRT) determination suitable for neonatal mass screening, and the preliminary steps towards its large-scale application are described. The method showed a highly significant correlation between blood spot and plasma values, and a study of plasma reference values in a population of 1,050 newborn infants demonstrated a log-normal distribution with a mean IRT concentration of 238.3 ng/ml. The results and their implications for neonatal mass screening are discussed.

Key-words

Cystic fibrosis Immunoreactive trypsin Neonatal test 

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References

  1. 1.
    Adrian T. E., Besterman H. S., Mallinson C. N., Galarotis C., Bloom S. R.: Plasma trypsin in the diagnosis of steatorrhea due to chronic pancreatitis - Gut19, A446, 1978.CrossRefGoogle Scholar
  2. 2.
    Antonozzi I., Del Castello P. G., Santagata G.: Loscreening neonatale di massa per le aminoacidopatie - Neuropsichiat. infant.203, 655, 1978.Google Scholar
  3. 3.
    Antonozzi I., Dominici R., Andreoli M., Monaco F.: Neonatal screening in Italy for congenital hypothyroidism and metabolic disorders: hyperphenylalaninemia, maple syrup urine disease and homocystinuria - J. endocr. Invest. (In press).Google Scholar
  4. 4.
    Applegarth D. A., Davidson A. G. F., Kirby L. T., Bridges M., Sorensen P., Wong L. T. K., Hardwicke D. F.: Dried-blood spot screening for cystic fibrosis - Lancetii, 1236, 1979.CrossRefGoogle Scholar
  5. 5.
    Bambule-Dick J., Gobelet C., Séchaud R., Magnenat P., Felber J.-P.: Preliminary results of blood immunoreactive trypsin levels in chronic pancreatitis - Irish J. med. Sci.146 (Suppl. 1), 3, 1977.Google Scholar
  6. 6.
    Crossley J. R., Elliott R. B., Smith P. A.: Dried-blood spot screening for cystic fibrosis in the newborn - Lanceti, 472, 1979.CrossRefGoogle Scholar
  7. 7.
    Dandona P., Hodson M., Bell J., Ramdial L., Batten J. C.: Serum-immunoreactivetrypsin concentrations in cystic fibrosis - Lanceti, 1032, 1979.CrossRefGoogle Scholar
  8. 8.
    Elias E., Redshaw M., Wood T.: Diagnostic importance of changes in circulating concentrations of immunoreactive trypsin - Lancetii, 66, 1977.CrossRefGoogle Scholar
  9. 9.
    Grant E. L., Leavenworth R. S.: Statistical quality control - McGraw-Hill Co., 1980; 5th edition.Google Scholar
  10. 10.
    King D. N., Heeley A. F., Walsh M. P., Kuzemko J. A.: Sensitive trypsin for dried-blood specimens as a screening procedure for early detection of cystic fibrosis - Lancetii, 1217, 1979.CrossRefGoogle Scholar
  11. 11.
    Kollberg M., Hellsing K.: Screening for cystic fibrosis by analysis of albumin in meconium - Acta paediat. scand.64, 477, 1975.PubMedCrossRefGoogle Scholar
  12. 12.
    Koop H., Arnild R., Lankisch P. G., Creutzfeldt W.: Trypsin radioimmunoassay in the diagnosis of chronic pancreatitis - In: XI Symposium of the European Pancreatic Club, Zürich, 1978. (Abstract).Google Scholar
  13. 13.
    Lake-Bakaar G., McKavanagh S., Wood T., Redshaw M. R., Summerfield J. A.: Assessment of the value of changes in serum concentration of immunoreactive trypsin after a Lundh meal - Gut19, A446, 1978.Google Scholar
  14. 14.
    Mastella G.: Terapia e prevenzione della fibrosi cistica - In: Atti del XXXVIII Congresso Nazionale della Società Italiana di Pediatria. Bari, 23–26 ottobre 1976; p. 87.Google Scholar
  15. 15.
    Stanbury J. B., Wyngaarden J. B., Fredrickson D. S.: The metabolic basis of inherited disease - McGraw-Hill Co., 1978; 4th edition, p. 1683.Google Scholar
  16. 16.
    Wood T. P., Redshaw M. R., Rousell R. H.: Units for immunoreactive trypsin - Lanceti, 781, 1979.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Roberto Dominici
    • 1
  • Fabrizio Monaco
    • 1
  • Silvana Morano
    • 2
  • Italo Antonozzi
    • 2
  1. 1.Istituto di Neuropsichiatria Infantile, Centro delia TiroideUniversità degli Studi di RomaItalia
  2. 2.Centro di Medicina Sociale per le Oligofrenie Dismetaboliche Istituto di Neuropsichiatria InfantileUniversità degli Studi di RomaRomaItalia

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