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Serum 25-hydroxy vitamin D in normal and osteomalacic subjects: A comparison of two assay techniques

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Summary

Two techniques for the assay of 25-(OH) votamin D are compared; one assay directly measures 25-(OH) vitamin D in serum, the other includes a chromatographic separation of serum 25-(OH) vitamin D from other vitamin D metabolites before radioassay.

Serum 25-(OH) vitamin D levels in normal and osteomalacic subjects are reported. Values in osteomalacia are low compared with control subjects.

Seasonal variation in 25-(OH) vitamin D level is demonstrable by both techniques.

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Authors and Affiliations

  1. The Department of Metabolism and Renal Diseases, St. Vincent's Hospital, Dublin

    B. Murray & R. Freaney

  2. Department of Medicine and Therapeutics, University College, Dublin

    B. Murray & R. Freaney

Authors
  1. B. Murray
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  2. R. Freaney
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Murray, B., Freaney, R. Serum 25-hydroxy vitamin D in normal and osteomalacic subjects: A comparison of two assay techniques. I.J.M.S. 148, 15–19 (1979). https://doi.org/10.1007/BF02938042

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