Serum 25-hydroxy vitamin D in normal and osteomalacic subjects: A comparison of two assay techniques

  • B. Murray
  • R. Freaney


Two techniques for the assay of 25-(OH) votamin D are compared; one assay directly measures 25-(OH) vitamin D in serum, the other includes a chromatographic separation of serum 25-(OH) vitamin D from other vitamin D metabolites before radioassay.

Serum 25-(OH) vitamin D levels in normal and osteomalacic subjects are reported. Values in osteomalacia are low compared with control subjects.

Seasonal variation in 25-(OH) vitamin D level is demonstrable by both techniques.


Osteomalacia Direct Assay Dihydrotachysterol Radioligand Receptor Assay Cytosol Binding Protein 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • B. Murray
    • 1
    • 2
  • R. Freaney
    • 1
    • 2
  1. 1.The Department of Metabolism and Renal DiseasesSt. Vincent's HospitalDublin
  2. 2.Department of Medicine and TherapeuticsUniversity CollegeDublin

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