Abstract
A procedure was developed for satisfactory freeze-sectioning of root tips. The use of Ca formol-fixed material kept and frozen in Holt's syrup is recommended. The existence and different localization of 2 fractions of non—specific esterase was verified in root tips ofVicia faba. The same results were revealed in fixed and unfixed material. The dynamics ofin situ reaction was followed with respect to optimal incubation time. The results with substrates of different chain length support the existence of 2 fraction of the studied enzyme, none of which, concerning substrate specificity, is a lipase. It follows from the present studies inVicia faba and other species (Cucurbita pepo, Lupinus albus, Pisum sativum Zea mays), that non-specific esterase localization is not directly given by histogenesis.
Abstract
V této práci byl vypracován postup pro získávání dobrých zmrazených řezů ze špiček klíčních kořenůVicia faba i jiných druhů rostlinCucurbita pepo, Lupinus albus, Pisum sativum, Zea mays). Spolehlivě lze použít Ca formolem fixovaného materiálu praného a řezaného v Holtově sirupu, lze však použít i materiálu nefixovaného, řezaného v H2O. UVicia byla ověřena existence a odlišná lokalisace 2 frakcí nespecifické esterasy. Výsledky u fixovaných i nefixovaných objektů byly stejné. Používaný postup se ukázal spolehlivý a nebyla zjištěna větší variabilita objektu. Rovněž uVicia byla sledována dynamika reakce in situ v souvislosti s optimální dobou inkubace. Existence 2 různých frakcí nespecifické esterasy byla zde ověřena použitím substrátů o různé délce řetězce. Z hlediska substrátové specificity není uVicia prokazovaný enzym lipasa. Z nálezů frakcí nespecifické esterasy uVicia i ostatních sledovaných objektů (Cucurbita, Lupinus, Pisum, Zea) plyne, že jejich lokalisace není jednoznačně dána histogenesí. Důsledně respektovat vývojově anatomické hledisko je conditio sine qua non jakékoliv srovnávací studie lokalisace enzymů.
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References
Abe, M., Kramer, S. P., Seligman, A. M.: The histochemical demonstration of pancreatic like lipase and comparison with the distribution of esterase.—J. Histochem. Cytochem.12: 364 to 383, 1964.
Adámková, A., Beneš, K.: Position and extent of the elongation zone in the root tip of the broad beanVicia faba L.—Biol. Plant.8: 427–430, 1966.
Baker, J. R.: Principles of biological microtechnique.—Methuen, London, 1958,
Beneš, K.: Cytological effect of low temperatures.—Folia biol.4: 244–245, 1958.
Beneš, K.: The cytomorphological effects of low temperatures studied by temperated fixatives. —Biol. Plant.1: 93–98, 1959.
Beneš, K.: Gistochimičeskoje dokazatel'stvo esterazy azokopuljacyonnym metodom v korněvoj verchuške bobaVicia faba L. [Histochemical demonstration of esterase in the root tip of Vicia faba L. with azo-coupling method.]—Biol. Plant.4: 211–219, 1962a.
Beneš, K.: Lokalisace nespecifické esterasy v kořenové špičce. Příspěvek k histochmickému studiu diferenciace pletiv. [The localization of non-specific esterase in root tip. A contribution to the histochemical study of tissue differentiation.]—CSc. thesis, Inst. of Exper. Botany, Praha, 1962b.
Benš, K.: Application of histo-and cytochemical methods to studying the differentiating apical meristem.—In: Differentiation of apical meristems and some problem of ecological regulation of development of plants. (A Symposium, 1964)—Academia, Praha, 1966.
Beneš, K.: On the stainability of plant cell walls with alcian blue.—Biol. Plant.10: 334 to 346, 1968.
Beneš, K.: Kořenová špička, její anatomie a genese pletiv kořene. [Root tip, its anatomy and root histogenesis.]—Biol. Listy35: 275–284, 1970.
Beneš, K., Opatrná, J.: Localization of acid phosphatase in the differentiating root meristem.— Biol. Plant.6: 8–16, 1964.
Beneš, K., Lojda, Z., Hořavka, B.: A contribution to the histochemical demonstration of some hydrolytic and oxidative enzymes in plants.—Histochemie2: 313–320, 1961.
Bünning, E.: Weitere Untersuchungen über die Differenzierungsvorgänge in Wurzeln.— Z. Bot.40: 385–406, 1952.
Danielli, J. F. (ed.): General cytochemical methods I.—Acad. Press, New York, 1958.
v. Deimling, O., Neuschäfer-Rube, G., Noltenius, H.: Methodische Untersuchungen zur quantitativen und histologischen Verteilung der alkalischen Phosphatase in den Hauptstücken der Rattenniere.—Histochemie8: 183–199, 1967.
Eränko, O., Kokko, A., Söderholm, U.: Histochemical evidence of three type of esterases in the adrenal medulla of the rat.—Histochemie2: 383–388, 1962.
Esau, K.: Plant Anatomy.—Wiley, New York, 1953.
Felgenhauer, K., Glenner, G. G.: Quantitation of tissue—bound renal aminopeptidase by a microdensitometric technique.—J. Histochem. Cytochem.14: 53–63, 1966.
Gahan, P. B.: Histochemical evidence for the presence of lysosome-like particles in root meristem cells ofVicia faba.—J. exper. Bot.16: 350–356, 1965.
Gahan, P. B., Maple, A. J.: The behaviour of lysosome—like particles during cell differentiation. —J. exp. Bot.17: 151–155, 1966.
Gahan, P. B., McLean, J., Kalina, M., Sharma, W.: Freezing-sectioning of plant tissues. The technique and its use in plant histochemistry.—J. exp. Bot.18: 151–159, 1967.
Hall, J. L., Butt, V. S.: Localization and kinetic properties of β-glycerophosphatase in barley roots.—J. exp. Bot.19: 276–287, 1968.
Holt, S. J., Hicks, R. M. Studies in formalin fixation for electron microscopy and cytochemical staining purposes.—J. biophys. biochem. Cytol.11: 31–45, 1961.
Holt, S. J., Hobbiger, E. E., Pawan, G. L. S: Preservation of integrity of rat tissues for cytochemical staining purposes.—J. biophys. biochem. Cytol.7: 383–386, 1960.
Hopsu, V. K., Glenner, G. G.: A histochemical enzyme kinetic system applied to the trypin-like amidase and esterase activity in human mast cells.—J. Cell Biol.17: 503–520, 1963.
Hopwood, D.: Fixatives an fixation: a review.—Histochem. J.1: 323, 1969.
Jensen, W. A.: Relation of primary cell wall formation to cell development in plants.—In:Rudnick, D. (ed.): Synthesis of molecular and cellular structure (19th Symp. Soc. Study Devel. Growth).—Ronald, New York, 1961.
De Jong, D. W.: Histochemical demonstration of extracellular distribution of acid phosphatase in onion roots.—Fyton22: 141–146, 1965.
Läuchli, A.: Histochemische Darstellung der sauren Phosphatase in pflanzlichen Kryostatschnitten. —Naturwiss.53: 533, 1966a.
Läuchli, A.: Cryostat technique for fresh plant tissues and its application in enzyme histochemistry. —Planta70: 13–25, 1966b.
Livingston, D. C., Coombs, M. M., Franks, L. M., Maggi, V., Gahan, P. B.: A lead phthalocyanin method for the demonstration of acid hydrolyses in plant and animal tissues.— Histochemie18: 48, 1969.
Lojda, Z.: Fixation in histochemistry.—Folia morphol.13: 65–83, 1965.
Meany, A., Gahan, P. B., Maggi, V.: Effect of triton X-100 on acid phosphatases with different substrate epecificities.—Histochemie11: 280, 1967.
Sahulka, J., Beneš, K.: Fraction of non-specific esterase in root tip ofVicia faba L. revealed by disc electrophoresis in acrylamide gel.—Biol. Plant.11: 23–33, 1969.
Thielke, Ch.: Enzymmuster im Wurzelmeristem.—Planta68: 371–374, 1966.
Walek-Czernecka, A.: Histochemical demonstration of some hydrolytic enzymes in the spherosomes of plant cells.—Acta. Soc. Botan. Polon.34: 573–588, 1965.
Wolman, M., Bubis, J. J., Wiener, H.: Histochemical study of the nature of the asymmetry of cell membranes.—Histochemie9: 1–12, 1967.
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Beneš, K. Histogenesis and localization of non-specific esterase in root tip. Biol Plant 13, 110–121 (1971). https://doi.org/10.1007/BF02936827
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DOI: https://doi.org/10.1007/BF02936827