Advertisement

Biologia Plantarum

, Volume 13, Issue 2, pp 110–121 | Cite as

Histogenesis and localization of non-specific esterase in root tip

  • K. Beneš
Article

Abstract

A procedure was developed for satisfactory freeze-sectioning of root tips. The use of Ca formol-fixed material kept and frozen in Holt's syrup is recommended. The existence and different localization of 2 fractions of non—specific esterase was verified in root tips ofVicia faba. The same results were revealed in fixed and unfixed material. The dynamics ofin situ reaction was followed with respect to optimal incubation time. The results with substrates of different chain length support the existence of 2 fraction of the studied enzyme, none of which, concerning substrate specificity, is a lipase. It follows from the present studies inVicia faba and other species (Cucurbita pepo, Lupinus albus, Pisum sativum Zea mays), that non-specific esterase localization is not directly given by histogenesis.

Keywords

Colour Intensity Central Cylinder Enzyme Localization Optimal Incubation Time Specific Esterase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Histogenese a lokalisace nespecifické esterázy v kořenové špiěce

Abstract

V této práci byl vypracován postup pro získávání dobrých zmrazených řezů ze špiček klíčních kořenůVicia faba i jiných druhů rostlinCucurbita pepo, Lupinus albus, Pisum sativum, Zea mays). Spolehlivě lze použít Ca formolem fixovaného materiálu praného a řezaného v Holtově sirupu, lze však použít i materiálu nefixovaného, řezaného v H2O. UVicia byla ověřena existence a odlišná lokalisace 2 frakcí nespecifické esterasy. Výsledky u fixovaných i nefixovaných objektů byly stejné. Používaný postup se ukázal spolehlivý a nebyla zjištěna větší variabilita objektu. Rovněž uVicia byla sledována dynamika reakce in situ v souvislosti s optimální dobou inkubace. Existence 2 různých frakcí nespecifické esterasy byla zde ověřena použitím substrátů o různé délce řetězce. Z hlediska substrátové specificity není uVicia prokazovaný enzym lipasa. Z nálezů frakcí nespecifické esterasy uVicia i ostatních sledovaných objektů (Cucurbita, Lupinus, Pisum, Zea) plyne, že jejich lokalisace není jednoznačně dána histogenesí. Důsledně respektovat vývojově anatomické hledisko je conditio sine qua non jakékoliv srovnávací studie lokalisace enzymů.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abe, M., Kramer, S. P., Seligman, A. M.: The histochemical demonstration of pancreatic like lipase and comparison with the distribution of esterase.—J. Histochem. Cytochem.12: 364 to 383, 1964.CrossRefGoogle Scholar
  2. Adámková, A., Beneš, K.: Position and extent of the elongation zone in the root tip of the broad beanVicia faba L.—Biol. Plant.8: 427–430, 1966.CrossRefGoogle Scholar
  3. Baker, J. R.: Principles of biological microtechnique.—Methuen, London, 1958,Google Scholar
  4. Beneš, K.: Cytological effect of low temperatures.—Folia biol.4: 244–245, 1958.Google Scholar
  5. Beneš, K.: The cytomorphological effects of low temperatures studied by temperated fixatives. —Biol. Plant.1: 93–98, 1959.CrossRefGoogle Scholar
  6. Beneš, K.: Gistochimičeskoje dokazatel'stvo esterazy azokopuljacyonnym metodom v korněvoj verchuške bobaVicia faba L. [Histochemical demonstration of esterase in the root tip of Vicia faba L. with azo-coupling method.]—Biol. Plant.4: 211–219, 1962a.CrossRefGoogle Scholar
  7. Beneš, K.: Lokalisace nespecifické esterasy v kořenové špičce. Příspěvek k histochmickému studiu diferenciace pletiv. [The localization of non-specific esterase in root tip. A contribution to the histochemical study of tissue differentiation.]—CSc. thesis, Inst. of Exper. Botany, Praha, 1962b.Google Scholar
  8. Benš, K.: Application of histo-and cytochemical methods to studying the differentiating apical meristem.—In: Differentiation of apical meristems and some problem of ecological regulation of development of plants. (A Symposium, 1964)—Academia, Praha, 1966.Google Scholar
  9. Beneš, K.: On the stainability of plant cell walls with alcian blue.—Biol. Plant.10: 334 to 346, 1968.Google Scholar
  10. Beneš, K.: Kořenová špička, její anatomie a genese pletiv kořene. [Root tip, its anatomy and root histogenesis.]—Biol. Listy35: 275–284, 1970.Google Scholar
  11. Beneš, K., Opatrná, J.: Localization of acid phosphatase in the differentiating root meristem.— Biol. Plant.6: 8–16, 1964.CrossRefGoogle Scholar
  12. Beneš, K., Lojda, Z., Hořavka, B.: A contribution to the histochemical demonstration of some hydrolytic and oxidative enzymes in plants.—Histochemie2: 313–320, 1961.CrossRefPubMedGoogle Scholar
  13. Bünning, E.: Weitere Untersuchungen über die Differenzierungsvorgänge in Wurzeln.— Z. Bot.40: 385–406, 1952.Google Scholar
  14. Danielli, J. F. (ed.): General cytochemical methods I.—Acad. Press, New York, 1958.Google Scholar
  15. v. Deimling, O., Neuschäfer-Rube, G., Noltenius, H.: Methodische Untersuchungen zur quantitativen und histologischen Verteilung der alkalischen Phosphatase in den Hauptstücken der Rattenniere.—Histochemie8: 183–199, 1967.CrossRefPubMedGoogle Scholar
  16. Eränko, O., Kokko, A., Söderholm, U.: Histochemical evidence of three type of esterases in the adrenal medulla of the rat.—Histochemie2: 383–388, 1962.CrossRefPubMedGoogle Scholar
  17. Esau, K.: Plant Anatomy.—Wiley, New York, 1953.Google Scholar
  18. Felgenhauer, K., Glenner, G. G.: Quantitation of tissue—bound renal aminopeptidase by a microdensitometric technique.—J. Histochem. Cytochem.14: 53–63, 1966.CrossRefPubMedGoogle Scholar
  19. Gahan, P. B.: Histochemical evidence for the presence of lysosome-like particles in root meristem cells ofVicia faba.—J. exper. Bot.16: 350–356, 1965.CrossRefGoogle Scholar
  20. Gahan, P. B., Maple, A. J.: The behaviour of lysosome—like particles during cell differentiation. —J. exp. Bot.17: 151–155, 1966.CrossRefGoogle Scholar
  21. Gahan, P. B., McLean, J., Kalina, M., Sharma, W.: Freezing-sectioning of plant tissues. The technique and its use in plant histochemistry.—J. exp. Bot.18: 151–159, 1967.CrossRefGoogle Scholar
  22. Hall, J. L., Butt, V. S.: Localization and kinetic properties of β-glycerophosphatase in barley roots.—J. exp. Bot.19: 276–287, 1968.CrossRefGoogle Scholar
  23. Holt, S. J., Hicks, R. M. Studies in formalin fixation for electron microscopy and cytochemical staining purposes.—J. biophys. biochem. Cytol.11: 31–45, 1961.PubMedCentralCrossRefPubMedGoogle Scholar
  24. Holt, S. J., Hobbiger, E. E., Pawan, G. L. S: Preservation of integrity of rat tissues for cytochemical staining purposes.—J. biophys. biochem. Cytol.7: 383–386, 1960.PubMedCentralCrossRefPubMedGoogle Scholar
  25. Hopsu, V. K., Glenner, G. G.: A histochemical enzyme kinetic system applied to the trypin-like amidase and esterase activity in human mast cells.—J. Cell Biol.17: 503–520, 1963.PubMedCentralCrossRefPubMedGoogle Scholar
  26. Hopwood, D.: Fixatives an fixation: a review.—Histochem. J.1: 323, 1969.CrossRefPubMedGoogle Scholar
  27. Jensen, W. A.: Relation of primary cell wall formation to cell development in plants.—In:Rudnick, D. (ed.): Synthesis of molecular and cellular structure (19th Symp. Soc. Study Devel. Growth).—Ronald, New York, 1961.Google Scholar
  28. De Jong, D. W.: Histochemical demonstration of extracellular distribution of acid phosphatase in onion roots.—Fyton22: 141–146, 1965.Google Scholar
  29. Läuchli, A.: Histochemische Darstellung der sauren Phosphatase in pflanzlichen Kryostatschnitten. —Naturwiss.53: 533, 1966a.CrossRefPubMedGoogle Scholar
  30. Läuchli, A.: Cryostat technique for fresh plant tissues and its application in enzyme histochemistry. —Planta70: 13–25, 1966b.CrossRefPubMedGoogle Scholar
  31. Livingston, D. C., Coombs, M. M., Franks, L. M., Maggi, V., Gahan, P. B.: A lead phthalocyanin method for the demonstration of acid hydrolyses in plant and animal tissues.— Histochemie18: 48, 1969.CrossRefPubMedGoogle Scholar
  32. Lojda, Z.: Fixation in histochemistry.—Folia morphol.13: 65–83, 1965.Google Scholar
  33. Meany, A., Gahan, P. B., Maggi, V.: Effect of triton X-100 on acid phosphatases with different substrate epecificities.—Histochemie11: 280, 1967.CrossRefPubMedGoogle Scholar
  34. Sahulka, J., Beneš, K.: Fraction of non-specific esterase in root tip ofVicia faba L. revealed by disc electrophoresis in acrylamide gel.—Biol. Plant.11: 23–33, 1969.CrossRefGoogle Scholar
  35. Thielke, Ch.: Enzymmuster im Wurzelmeristem.—Planta68: 371–374, 1966.CrossRefPubMedGoogle Scholar
  36. Walek-Czernecka, A.: Histochemical demonstration of some hydrolytic enzymes in the spherosomes of plant cells.—Acta. Soc. Botan. Polon.34: 573–588, 1965.CrossRefGoogle Scholar
  37. Wolman, M., Bubis, J. J., Wiener, H.: Histochemical study of the nature of the asymmetry of cell membranes.—Histochemie9: 1–12, 1967.CrossRefPubMedGoogle Scholar

Copyright information

© Institute of Experimental Botany 1971

Authors and Affiliations

  • K. Beneš
    • 1
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencePraha

Personalised recommendations