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Depolymerization of water soluble coal polymer from subbituminous coal and lignite by lignin peroxidase

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Abstract

Coal polymers, water soluble at pH 3.5, were prepared from North Dakota lignite and German subbituminous coal in 35–61% yield. Gel permeation chromatography showed a major component of relatively narrow molecular weight range >75,000. The material did not dialyze through a 12,000-14,000 MW cutoff membrane under several conditions. Minor amounts of smaller fragments were present, but monomeric components were not detected. Incubation of soluble polymer with lignin peroxidase ofPhanerochaete chrysosporium caused substantial disappearance of the high molecular weight polymer and formation of smaller amounts of both higher and lower molecular weight components, but not of monomeric compounds. Addition of veratryl alcohol enhanced depolymerization. Coal polymer competitively inhibited veratryl alcohol oxidation by lignin peroxidase.

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References

  1. Cohen, M. S., and Gabriele, P. D. (1982),Appl. Environ. Microbiol. 44, 23–27.

    CAS  Google Scholar 

  2. Scott, C. D., Strandberg, G. W., and Lewis, S. N. (1986),Biotechnol. Prog. 2, 131–139.

    CAS  Google Scholar 

  3. Strandberg, G. W. and Lewis, S. N. (1987),J. Ind. Microbiol. 1, 371–275.

    Article  CAS  Google Scholar 

  4. Wilson, B. W., Lewis, E. J., Stewart, D. L., and Li, S. M. (1985), pp. 31.1–31.8 in Proceedings of The Tenth Annual EPRI Contractors’ Conference on Clean Liquid and Solid Fuels, April 23-25,1985, Palo Alto, CA, Electric Power Research Institute, Palo Alto, CA.

  5. Ward, H. B. (1985),Sys. Appl. Microbiol. 6, 236–238.

    Google Scholar 

  6. Wilson, B. W., Bean, R. M., Franz, J. A., Thomas, B. L., Cohen, M. S., Aronson, H., and Gray, Jr., E. T. (1987),Energy & Fuels 1, 80–84.

    Article  CAS  Google Scholar 

  7. Pyne, J. W., Jr., Stewart, D. L., Fredrickson, J., and Wilson, B. W. (1987),Appl. Envion. Microbiol. 53, 2844–2848.

    CAS  Google Scholar 

  8. Wilson, B. W., Lewis, E. J., Stewart, D. L., Li, S. M., Bean, R. M., Chess, E. K., Pyne, J., Cohen, M., and Aronson, H. (1986), pp. IV88-IV98, in Proceedings of Department of Energy Coal Liquification Contractors’ Meeting, Pittsburgh, PA, November 19-21, 1985, NTIS, Springfield, VA.

  9. Tien, M. and Kirk, T. K. (1983),Science 221, 661–663.

    Article  CAS  Google Scholar 

  10. Glenn, J. K., Morgan, M. A., Mayfield, M. B., Kuwahara, M., and Gold, M. H. (1983),Biochem. Biophys. Res. Commun. 114, 1077–1083.

    Article  CAS  Google Scholar 

  11. Tien, M. and Kirk, T. K. (1984),Proc. Natl. Acad. Sci. USA 81, 2280–2284.

    Article  CAS  Google Scholar 

  12. Gold, M. H., Kuwahara, M., Chiu, A. A., and Glenn, J. K. (1984),Arch. Biochem. Biophys. 234, 353–362.

    Article  CAS  Google Scholar 

  13. Umezawa, T., Shimada, M., Higuchi, T., and Kusai, K. (1986),FEBS Lett. 205, 287–292.

    Article  CAS  Google Scholar 

  14. Umezawa, T. and Higuchi, T. (1987),Agric. Biol. Chem. 51, 2281–2284.

    CAS  Google Scholar 

  15. Haemmerli, S. D. Leisola, M. S. A., Sangïard, D., and Fiechter, A. (1986),J. Biol. Chem. 251, 6900–6903.

    Google Scholar 

  16. Hammel, K. W., Kalyanaraman, B., and Kirk, T. K. (1986),J. Biol. Chem. 261, 16948–16952.

    CAS  Google Scholar 

  17. Kersten, P. J., Tien, M., Kalyanaraman, B., and Kirk, T. K. (1985),J. Biol. Chem. 260, 2609–2612.

    CAS  Google Scholar 

  18. Hammel, K. E., Tien, M., Kalyanaraman, B., and Kirk, T. K. (1985),J. Biol. Chem. 260, 8348–8353.

    CAS  Google Scholar 

  19. Hammel, K. E., Kalyanaraman, B., and Kirk, T. K. (1986),Proc. Natl. Acad. Sci. USA 83, 3708–3712.

    Article  CAS  Google Scholar 

  20. Kirk, T. K., S., Croan, and Tien, M., Murtagh, K. E., and Farrell, R. L. (1986),Enzyme Microb. Technol. 8, 27–32.

    Article  CAS  Google Scholar 

  21. Archibald, F. S. and Fridovich, I. (1982),Arch. Biochem. Biophys. 214, 452–463.

    Article  CAS  Google Scholar 

  22. Glenn, J. K. and Gold, M. H. (1985),Arch. Biochem. Biophys. 242, 329–341.

    Article  CAS  Google Scholar 

  23. Johnson, M. J. (1949),J. Biol. Chem. 181, 707–711.

    CAS  Google Scholar 

  24. Beyer, M. (1987),Biotechnol. Lett. 9, 19–24.

    Article  CAS  Google Scholar 

  25. van Krevelen, D. W. (1950),Fuel 29, 269.

    Google Scholar 

  26. Jüntgen, H., in Biotechnologie im Steinkohlenbergbau, Steinkohlenbergbauverein Workshop Coal Recovery, Second Generation, Jan 1983, Essen, FRG, p. 28.

  27. Schutz, J. G. and Sabourin, T., US Patent 4,052,488 (1977).

  28. 28. Schultz, J. G. D., and Zajac, J., US Patent 4,235,728 (1980).

  29. Juettner, B., Smith, R. C. and Howard, H. C. (1935),J. Am. Chem. Soc. 57, 2322–2326.

    Article  CAS  Google Scholar 

  30. Chowdhury, J. K., and Biswas, A. B. (1942),J. Indian Chem. Soc. 19, 289–298.

    CAS  Google Scholar 

  31. Charmbury, H. B., Eckerd, J. W., La Torre, J. S., and Kinney, C. R. (1945),J. Am. Chem. Soc., 625#628.

  32. Wondrack, L., Szanto, M., and Wood, W. A. (1987) (unpublished observations).

  33. Harvey, P. J., Shoemaker, H. E., and Palmer, J. M. (1986),FEBS Lett. 195, 242–246.

    Article  CAS  Google Scholar 

  34. Renganathan, V., Miki, K., and Gold, M. H. (1985),Arch. Biochem. Biophys. 241, 304–314.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Salk Institute Biotechnology, Industrial Associates, Inc., PO Box 85200, 92138, San Diego, CA

    Lillian Wondrack, Maria szanto & Willis A. wood

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  1. Lillian Wondrack
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  2. Maria szanto
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  3. Willis A. wood
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Wondrack, L., szanto, M. & wood, W.A. Depolymerization of water soluble coal polymer from subbituminous coal and lignite by lignin peroxidase. Appl Biochem Biotechnol 20, 765–780 (1989). https://doi.org/10.1007/BF02936524

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