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Butyrate production from carbon monoxide byButyribacterium methylotrophicum

  • R. M. Worden
  • A. J. Grethlein
  • J. G. Zeikus
  • R. Datta
Session 3 Bioengineering research

Abstract

Carbon monoxide is produced in high concentrations by gasification of coal or biomass, and is a potentially inexpensive feedstock for biological processes. A number of anaerobic microorganisms metabolize carbon monoxide, with acetate, hydrogen, or methane being the primary reduced products. The CO strain ofButyribacterium methylotrophicum was previously shown to grow on carbon monoxide as the sole carbon and energy source, with acetate being the primary product. This paper demonstrates that by modifying culture conditions, the carbon and electron flow ofB. methylotrophicum can be manipulated to yield butyrate as the major product. A butyrate concentration of 6 g/L was obtained in batch culture with continuous addition of 100% carbon monoxide. The significance of this fermentation for fuels and chemicals production from carbon monoxide is discussed.

Index Entries

Carbon monoxide butyrate fermentation masstransfer Butyribacterium methylotrophicum 

References

  1. 1.
    Busche, R. M. (1985),Biotechnol. Prog. 1, 165.CrossRefGoogle Scholar
  2. 2.
    Keim, W. (1987),ACS Symposium Series 328, American Chemical Society, Washington, DC, 1.Google Scholar
  3. 3.
    Zeikus, J. G. (1983),Adv. Microb. Physiol. 24, 215.CrossRefGoogle Scholar
  4. 4.
    Williams, E., Colby, J., Lyons, C. M., and Bell, J. (1986),Biotechnology and Genetic Engineering Reviews, volume 4, Intercept, Ponteland, 169.Google Scholar
  5. 5.
    Zeikus, J. G., Lynd, L. H., Thompson, T. E., Krzycki, J. A., Weimer, P. J., and Hegge, P. W. (1980),Current Microbiol. 3, 381.CrossRefGoogle Scholar
  6. 6.
    Moench, T. T. and Zeikus, J. G. (1983),Current Microbiol. 9, 151.CrossRefGoogle Scholar
  7. 7.
    Kerby, R. and Zeikus, J. G. (1987),J. Bacter. 169, 2063.Google Scholar
  8. 8.
    Lynd, L., Kerby, R., and Zeikus, J. G. (1982),J. Bacteriol. 149, 255.Google Scholar
  9. 9.
    Zeikus, J. G., Kerby, R., and Krzycki, J. A. (1985),Science 227, 1167.CrossRefGoogle Scholar
  10. 10.
    Datta, R. (1982),Biotech. Bioeng. Symp. Ser. 12, 177.Google Scholar
  11. 11.
    Erickson, L. E., Minkevich, I. G., and Eroshin, V. K. (1978),Biotech. Bioeng. 20, 1595.CrossRefGoogle Scholar
  12. 12.
    Erickson, L. E. and Oner, M. D. (1983),Ann. N Y Acad. Sci. 413, 99.CrossRefGoogle Scholar
  13. 13.
    Leudeking, R. and Piret, E. L. (1959),J. Biochem. Microbiol. Technol. Eng. 1, 393.CrossRefGoogle Scholar
  14. 14.
    Erickson, L. E., Selga, S. E., and Viesturs, U. E. (1978),Biotech. Bioeng. 20, 1623.CrossRefGoogle Scholar
  15. 15.
    Roels, J. A. (1983),Energetics and Kinetics in Biotechnology, Elsevier, New York, p. 40.Google Scholar
  16. 16.
    Jones, R. P. and Greenfield, P. F. (1982),Enzyme Microb. Technol. 4, 210.CrossRefGoogle Scholar
  17. 17.
    Stumm, W. and Morgan, J. J. (1970),Aquatic Chemistry, Wiley, New York, p. 127.Google Scholar
  18. 18.
    Burden, R. L., Faires, J. D., and Reynolds, A. C. (1981),Numerical Analysis, 2nd ed., Prindle, Weber and Schmidt, Boston, p. 127.Google Scholar
  19. 19.
    Lee, Y. H. and Luk, S. (1983),Ann. Rep. Verm. Proc. 6, 121.Google Scholar
  20. 20.
    National Research Council (1928),International Critical Tables, vol. 3, McGraw-Hill, New York, 254.Google Scholar
  21. 21.
    Wilke, C. R. and Chang, P. (1955),AICHE J. 1, 264.CrossRefGoogle Scholar
  22. 22.
    Danckwerts, P. V. (1955),AICHE J. 1, 456.CrossRefGoogle Scholar
  23. 23.
    Agreda, V. H. (1988),Chemtech April, 250.Google Scholar
  24. 24.
    Reisman, H. B. (1988),Economic Analysis of Fermentation Processes, CRC Press, Boca Raton, FL, p. 24.Google Scholar
  25. 25.
    Datta, R. (1981),Biotechnol. Bioeng. Symp. Ser. 11, 521.Google Scholar
  26. 26.
    Meyer, C. L., Roos, J. W., and Papoutsakis, E. T. (1986),App. Microbiol. Biotechnol. 24, 159.Google Scholar
  27. 27.
    Lenz, R. W. (1988), Michigan State University, personal communication.Google Scholar

Copyright information

© Humana Press Inc. 1989

Authors and Affiliations

  • R. M. Worden
    • 1
  • A. J. Grethlein
    • 1
  • J. G. Zeikus
    • 2
  • R. Datta
    • 2
  1. 1.Department of Chemical EngineeringMichigan State UniversityEast Lansing
  2. 2.Michigan Biotechnology InstituteLansing

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