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Journal of Gastroenterology

, Volume 32, Issue 2, pp 200–205 | Cite as

Induction of nonspecific cross-reacting antigen mRNA by interferon-γ and anti-fibronectin receptor antibody in colon cancer cells

  • Yuji Hinoda
  • Tehzoh Saito
  • Hiroki Takahashi
  • Fumio Itoh
  • Masaaki Adachi
  • Kohzoh Imai
Alimentary Tract

Abstract

Nonspecific cross-reacting antigen (NCA), a member of the carcinoembryonic antigen (CEA) family, shows increased expression levels in colorectal cancer tissues. To elucidate the mechanism, we observed the effect of interon (IFN)-γ on the expression level of NCA mRNA in colon cancer cell lines by quantitative reverse transcriptase-polymerase chain reaction assay. IFN-γ induced NCA mRNA in three of four cell lines tested. The effect of anti-fibronectin receptor (FnR) antibody on the expression of NCA mRNA was then examined in the same manner. Colo201 and DLD-1 cells showed an increased expression level of NCA mRNA after stimulation with the antibody. On flow cytometry, FnR was expressed in only two, Colo201 and DLD-1, of the five cell lines tested. These findicate that IFN-γ and anti-FnR antibody induce NCA mRNA in cultured colon cancer cell lines, suggesting that inflammatory response and cell-to-extracellular matrix interaction may be related to the increased expression of NCA mRNA in colorectal cancers in vivo.

Key words

nonspecific cross-reacting antigen (NCA) carcinoembryonic antigen (CEA) interferon-γ antifibronectin antibody colon cancer cells 

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Yuji Hinoda
    • 1
  • Tehzoh Saito
    • 1
  • Hiroki Takahashi
    • 1
  • Fumio Itoh
    • 1
  • Masaaki Adachi
    • 1
  • Kohzoh Imai
    • 1
  1. 1.First Department of Internal MedicineSapporo Medical UniversitySapporoJapan

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