Folia Microbiologica

, Volume 36, Issue 1, pp 67–70 | Cite as

Isolation method for lysine-excreting mutants ofArthrobacter globiformis

  • S. K. Sen


To improve the yield of lysine by the isolate, auxotrophic mutants were isolated. Among the mutants, only one auxotrophic mutant required vitamin B12. This mutant producedα-alanine. About 200 mutants resistant to the lysine analog S-(2-aminoethyl)-l-cysteine were isolated and some of them produced well above the wild type.


Lysine Auxotrophic Mutant Lysine Producer Lysine Analog Arthrobacter Globiformis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ajinomoto Co.: Production ofl-lysine by fermentation.Brit.Pat. 1 258 380;Chem.Abstr. 76, 84530c (1971).Google Scholar
  2. Ajinomoto Co.:l-Lysine production.Brit. Pat. 1 425 648;Microb.Abstr. 12 (11),3 (1976).Google Scholar
  3. Kaudewitz F., Vielmetter W., Friedrich-Freksa H.: Mutagene Wirkung des Zerfalles von radioaktiven Phosphor nach Einbau von Zellen vonEscherichia coli.Z.Naturforsch. 13B, 793 (1958).Google Scholar
  4. Kinoshita S., Nakayama K., Kitada S.:l-Lysine production by microbial auxotroph.J.Gen.Appl.Microbiol. 4, 128 (1958).CrossRefGoogle Scholar
  5. Lederberg J., Lederberg E.M.: Replica plating technique and indirect selection of bacterial mutants.J.Bacteriol. 63, 399 (1952).PubMedGoogle Scholar
  6. Mukula R., Finnerty W.R.: Microbial assimilation of hydrocarbons. I. Fatty acids derived fromn-alkanes.J.Bacteriol. 95, 2108. (1968).Google Scholar
  7. Sano K., Shiio I.: Microbial production ofl-lysine. IV. Selection of lysine producing mutants fromBrevibacterium flavum by detecting threonine sensitivity or halo-forming method.J.Gen.Appl. Microbiol. 17, 97 (1971).CrossRefGoogle Scholar
  8. Sen S.K., Chatterjee M., Chatterjee S.P.: Microbial production of amino acids from hydrocarbon enrichment, isolation and selection of microorganisms.Biol.Bul.India 4, 131 (1982).Google Scholar
  9. Sen S.K.: Isolation method of lysine overproducers from hydrocarbon utilizing microorganisms.Acta Biotechnol. 5, 379 (1985).CrossRefGoogle Scholar
  10. Tosaka O., Koichi T., Hirose Y.: Production ofl-lysine by leucine auxotrophs derived from AEC-resistant mutant ofBrevibacterium lactofermentum.Agric.Biol.Chem. 42, 1181 (1978).Google Scholar
  11. Tosaka O., Takinami K.: Lysine, pp. 157–172 inK. Aida, I. Chibata, K. Nakayama, K. Takinami, H. Yamada:Biotechnology of Amino Acid Production. Elsevier, Amsterdam-Oxford-New-York-Tokyo 1986.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1991

Authors and Affiliations

  • S. K. Sen
    • 1
  1. 1.Microbiology Laboratory, Department of Botany, School of Life ScienceVisva-Bharati University, SantiniketanWest BengalIndia

Personalised recommendations