Journal of the American Oil Chemists Society

, Volume 62, Issue 6, pp 1016–1021 | Cite as

Continuous hydrolysis of olive oil by lipase in microporous hydrophobic membrane bioreactor

  • Mohammad Mozammel Hoq
  • Tsuneo Yamane
  • Shoichi Shimizu
  • Tadashi Funada
  • Shiro Ishida


Continuous hydrolysis of olive oil byCandida cylindracea’s lipase was studied in a microporous hydrophobic membrane bioreactor. Olive oil and buffer solution, fed continuously through two compartments partitioned by membrane, caused reaction at the interface of lipase-adsorbed membrane and buffer solution. Fatty acid was obtained in a single phase without being mixed with components of other phases. At all mean residence times, countercurrent flow mode was superior to cocurrent one. The lipase was adsorbed onto the membrane, and its adsorption was suggested to be partially specific from the experiments with enzymes having various levels of purity. The percent hydrolysis depended hyperbolically on the interfacial enzyme concentration. The hydrolysis seemed to be limited by diffusion of fat or fatty acid through the micropores of the membrane at higher interfacial enzyme concentrations. The lipase was stabilized significantly by glycerol added to the buffer solution. Satisfactory performance of the membrane bioreactor was obtained in a longterm continuous operation which lasted for 24 days by feeding buffer-glycerol (18.0%) solution over the adsorbed lipase. The operational half-life of the adsorbed enzyme was 15 days at 40 C.


Lipase Enzyme Solution Glycerol Concentration Membrane Bioreactor Glycerol Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Stirton, A.J., In Bailey’s Industrial Oil and Fat Products (D. Swern, ed.) John Wiley and Sons, New York, 1964, p. 940.Google Scholar
  2. 2.
    Miyoshi Oil and Fat Co., Japan, Jap. Chem. Week (1981).Google Scholar
  3. 3.
    Sonntag, N.O.V., JAOCS 56:729A (1979).Google Scholar
  4. 4.
    Stansby, M.E., JAOCS 56:793A (1979).Google Scholar
  5. 5.
    Kosugi, Y., and H. Suzuki, J. Ferment. Technol. 61:287 (1983).Google Scholar
  6. 6.
    Ishida, S., K. Koyama, M. Nishimura and T. Funada, Abstract of Annual Meeting of Japan Oil Chemists’ Society, p. 13 (1983).Google Scholar
  7. 7.
    Kobayashi, T., S. Mukataka, H. Kataoka and J. Takahashi, Abstract of Annual Meeting of the Soc. Ferment. Technol. Japan, p. 21 (1983).Google Scholar
  8. 8.
    Linfield, W.M., D.J. O’Brien, S. Serota and R.A. Barauskas, JAOCS 61:1067 (1984).CrossRefGoogle Scholar
  9. 9.
    Lieberman, R.B., and D.F. Ollis, Biotechnol. Bioeng. 17:1401 (1975).CrossRefGoogle Scholar
  10. 10.
    Kilara, A., K.M. Shahani and F.W. Wagner, Ibid 19:1703 (1977).CrossRefGoogle Scholar
  11. 11.
    Lavayre, J., and J. Bratti, Ibid. 24:1007 (1982).CrossRefGoogle Scholar
  12. 12.
    Kimura, Y., A. Tanaka, K. Sonomoto, T. Nihira and S. Fukui, Eur. J. Appl. Microbiol. Biotechnol. 17:107 (1983).CrossRefGoogle Scholar
  13. 13.
    Kosugi, Y., and H. Suzuki, J. Ferment. Technol. 51:895 (1973).Google Scholar
  14. 14.
    Bell, G., J.R. Todd, J.A. Blain, J.D.E. Patterson and C.E.L. Shaw, Biotechnol. Bioeng. 23:1703 (1981).CrossRefGoogle Scholar
  15. 15.
    Hoq, M. Mozammel, T. Yamane, S. Shimizu, T. Funada and S. Ishida, JAOCS 61:776 (1984).CrossRefGoogle Scholar
  16. 16.
    Hoq, M. Mozammel, H. Tagami, T. Yamane and S. Shimizu, Agric. Biol. Chem. 49:335 (1985).Google Scholar
  17. 17.
    Yamane, T., T. Funada and S. Ishida, J. Ferment. Technol. 60:517 (1982).Google Scholar
  18. 18.
    Murakami, S., T. Funada and S. Ishida, J. Jap. Oil Chem. Soc. 32:493 (1983).Google Scholar
  19. 19.
    Sugiura, M., and M. Isobe, Biochim. Biophys. Acta 397:412 (1975).Google Scholar
  20. 20.
    Macrea, A.R., “Microbial Enzymes and Biotechnology” (W.M. Fogarty, ed.) Applied Science Publishers, London & New York, 1983, Chap. 5.Google Scholar
  21. 21.
    Technical Service Bulletin, The microbial lipase ofCandida cylindracea, Nov. sp., Meito Sangyo Co. Ltd., Japan (1975).Google Scholar

Copyright information

© American Oil Chemists 1985

Authors and Affiliations

  • Mohammad Mozammel Hoq
    • 1
  • Tsuneo Yamane
    • 1
  • Shoichi Shimizu
    • 1
  • Tadashi Funada
    • 2
  • Shiro Ishida
    • 2
  1. 1.Laboratory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of AgricultureNagoya UniversityNagoyaJapan
  2. 2.Research Laboratory, Amagasaki WorksNippon Oil and Fats Co. Ltd.AmagasakiJapan

Personalised recommendations