Medical Oncology and Tumor Pharmacotherapy

, Volume 2, Issue 4, pp 261–267 | Cite as

Characterization of chronic lymphocytic leukemia (CLL) cells with low density of surface membrane bound immunoglobulins (smIg)

  • E. Kimby
  • B. Axelsson
  • M. Björkholm
  • J. Gordon
  • G. Holm
  • H. Mellstedt


The study consists of 6 CLL patients with leukemic blood lymphocytes lacking T-cell characteristics and with smIg on only a very small fraction of the cells detected by microscopy or FACS analysis after direct immunofluorescence (IFL) staining. Using B-cell specific monoclonal antibodies all leukemias were found to be of the B-cell type. SmIg, mainly of IgD-class and the monotypic light chain, was detected on a large number of cells in all cases when using direct IFL. No lymphocyte clone was judged to be of the pre-B-cell type or represented fully differentiated terminal B-cells. CLL cells from the individual cases probably represent intermediate B-cell maturation steps. The results lend no support to the suggestion that this subtype of CLL necessarily belongs to an early stage of differentiation just beyond the pre-B-cell level.

Key words

CLL Low density smIg Differentiation 


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Copyright information

© Humana Press Inc. 1985

Authors and Affiliations

  • E. Kimby
    • 1
  • B. Axelsson
    • 2
  • M. Björkholm
    • 1
  • J. Gordon
    • 5
  • G. Holm
    • 3
  • H. Mellstedt
    • 4
  1. 1.Department of Medicine, Division of HematologyDanderyd HospitalStockholmSweden
  2. 2.Department of ImmunologyStockholm UniversityStockholmSweden
  3. 3.Department of Clinical ImmunologyHuddinge HospitalStockholmSweden
  4. 4.Radiumhemmet and Department of Medical RadiobiologyKarolinska HospitalStockholmSweden
  5. 5.Department of ImmunologyUniversity of BirminghamBirminghamUK

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