Skip to main content
SpringerLink
Log in

Characterization of chronic lymphocytic leukemia (CLL) cells with low density of surface membrane bound immunoglobulins (smIg)

  • Articles
  • Published:
Medical Oncology and Tumor Pharmacotherapy Aims and scope Submit manuscript

Abstract

The study consists of 6 CLL patients with leukemic blood lymphocytes lacking T-cell characteristics and with smIg on only a very small fraction of the cells detected by microscopy or FACS analysis after direct immunofluorescence (IFL) staining. Using B-cell specific monoclonal antibodies all leukemias were found to be of the B-cell type. SmIg, mainly of IgD-class and the monotypic light chain, was detected on a large number of cells in all cases when using direct IFL. No lymphocyte clone was judged to be of the pre-B-cell type or represented fully differentiated terminal B-cells. CLL cells from the individual cases probably represent intermediate B-cell maturation steps. The results lend no support to the suggestion that this subtype of CLL necessarily belongs to an early stage of differentiation just beyond the pre-B-cell level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rai K R, Sawitsky A, Cronkite E P, Chanana A D, Levy R N, Pasternack B S: Clinical staging of chronic lymphocytic leukemia.Blood 46, 219 (1975).

    PubMed  CAS  Google Scholar 

  2. Simonsson B, Nilsson K:3H-thymidine uptake in chronic lymphocytic leukemia cells.Scand J Haemat 24, 169 (1980).

    PubMed  CAS  Google Scholar 

  3. Hernandez-Nieto L, Montserrat-Costa E, Muncunill J, Roznan C: Bone marrow patterns and clinical staging in chronic lymphocytic leukemia.Lancet i, 1269 (1977).

    Article  Google Scholar 

  4. Simonsson B, Terenius L, Nilsson K: Glucocorticoid receptors, clinical characteristics and implications for prognosis in chronic lymphocytic leukemia.Cancer 49, 2493 (1982).

    Article  PubMed  CAS  Google Scholar 

  5. Rudders R A: B lymphocyte subpopulations in chronic lymphocytic leukemia.Blood 47, 229 (1976).

    PubMed  CAS  Google Scholar 

  6. Kimby E, Mellstedt H, Björkholm M, Holm G: Surface immunoglobulin pattern of the leukemic cell population in chronic lymphocytic leukemia (CLL) in relation to disease activity (manuscript to be published).

  7. Robért K-H: Induction of monoclonal antibody synthesis in malignant human B cells by polyclonal B cell activators.Immun Rev 48, 123 (1979).

    Article  PubMed  Google Scholar 

  8. Ideström K, Kimby E, Björkholm M, Mellstedt H, Engstedt L, Gahrton G, Johansson B, Killander D, Robért K-H, Stalfelt A-M, Udén A-M, Wadman B, Wählby S: Treatment of chronic lymphocytic leukemia and well differentiated lymphocytic lymphoma with continuous low or intermittent high dose prednimustine versus chlorambucil/prednisolone.Eur J Cancer Clin Oncol 11, 1117 (1982).

    Article  Google Scholar 

  9. Holm G, Pettersson D, Mellstedt H, Hedfors E, Bloth B: Lymphocyte subpopulation in peripheral blood of healthy persons. Characterization by surface markers and lack of selection during purification.Clin Exp Immun 20, 443 (1975).

    Google Scholar 

  10. Pettersson D, Mellstedt H, Holm G: IgG on human blood lymphocytes studied by immunofluorescence.Scand J Haemat 8, 535 (1978).

    CAS  Google Scholar 

  11. Stashenko P, Nadler L M, Hardy R, Schlossman S F: Characterization of a B-lymphocyte-specific antigen.J Immun 125, 1678 (1980).

    PubMed  CAS  Google Scholar 

  12. Nadler L M, Stashenko P, Hardy R, Agthoven A, Terhorst C, Schlossman S F: Characterization of a B cell specific antigen (B2) distinct from B1.J Immun 126, 1941 (1981).

    PubMed  CAS  Google Scholar 

  13. Nadler L M, Anderson K C, Marti G, Bates M P, Park E K, Daley J F, Schlossman S F: B4, a human B lymphocyte associated antigen expressed on normal mitogen activated and malignant B lymphocytes.J Immun 131, 244 (1983).

    PubMed  CAS  Google Scholar 

  14. Nadler L M, Korsmeyer S J, Anderson K C, Boyd A W, Slaughenhoupt B, Park E, Jensen J, Coral F, Mayer R J, Sallan S E, Ritz J, Schlossman S F: B cell origin of non-T cell acute lymphoblastic leukemia. A model for discrete stages of neoplastic and normal pre-B cell differentiation.J Clin Invest 74, 332 (1984).

    Article  PubMed  CAS  Google Scholar 

  15. Second Int Congr Human Leukocyte Antigens. Boston, 17–20 September 1984.

  16. Ritz J, Pesando J M, Notis-McConarty J, Lazarus H, Schlossman S F: A monoclonal antibody to human acute lymphoblastic leukemia antigen.Nature 283, 583 (1980).

    Article  PubMed  CAS  Google Scholar 

  17. Anderson K C, Park E K, Bates M P, Leonard R C F, Hardy R, Schlossman S F, Nadler L M: Antigens on human plasma cells identified by monoclonal antibodies.J Immun 130, 1132 (1983).

    PubMed  CAS  Google Scholar 

  18. Anderson K C, Bates M P, Slaughenhoupt B, Schlossman S F, Nadler L M: A monoclonal antibody with reactivity restricted to normal and neoplastic plasma cells.J Immun 132, 3172 (1984).

    PubMed  CAS  Google Scholar 

  19. Gerdes J, Naeim M, Mason D Y, Stein H: Human complement (C3b) receptors defined by a mouse monoclonal antibody.Immunology 45, 645 (1982).

    PubMed  CAS  Google Scholar 

  20. Reinherz E L, Kung P C, Goldstein G, Schlossman S F: A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells.J Immun 123, 1312 (1979).

    PubMed  CAS  Google Scholar 

  21. Breard J, Reinherz E L, Kung P C, Goldstein G, Schlossman S F: A monoclonal antibody reactive with human peripheral blood monocytes.J Immun 124, 1943 (1980).

    PubMed  CAS  Google Scholar 

  22. Reinherz E L, Kung P C, Goldstein G, Levey R H, Schlossman S F: Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblast of T-cell lineage.Proc Natl Acad Sci U.S.A. 77, 1588 (1980).

    Article  PubMed  CAS  Google Scholar 

  23. Janossy G, Bollum F J, Bradstock K F, McMichael A, Rapson N, Greaves M F: Terminal transferasepositive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia.J Immun 123, 1525 (1979).

    PubMed  CAS  Google Scholar 

  24. Lindemalm C, Mellstedt H, Biberfeld P, Björkholm M, Christensson B, Holm G, Johansson B, Sundblad R: Blood and lymph node T lymphocyte subsets in non-Hodgkin lymphomas.Scand J Haemat 30, 68 (1983).

    PubMed  CAS  Google Scholar 

  25. Axelsson B, Hammerström S, Robertsson E S, Åman P, Mellstedt H, Perlmann P: The large sialoglycoprotein of human lymphocytes. Distribution on T- and B-lineage cells as revealed by a monospecific chicken antibody.Eur J Immun (in press).

  26. Tötterman T H, Nilsson K, Claesson L, Simonsson B, Åman P: Differentiation of chronic lymphocytic leukemia cellsin vitro. I. Phorbol ester induced changes in the synthesis of immunoglobulin and HLA-DR.Human Lymphocyte Differentiation 1, 13 (1981).

    Google Scholar 

  27. Slease R B, Wistar R, Scher I: Surface immunoglobulin density on human peripheral blood mononuclear cells.Blood 54, 72 (1979).

    PubMed  CAS  Google Scholar 

  28. Foa R, Catovsky D, Brozovic M, Marsh G, Ooyirilangkumaran T, Cherchi M, Gallton D: Clinical staging and immunological findings in chronic lymphocytic leukemia.Cancer 44, 483 (1979).

    Article  PubMed  CAS  Google Scholar 

  29. Dillman R O, Beauregard J C, Lea J, Green M, Sobol R, Roysten I: Chronic lymphocytic leukemia and other chronic lymphoid proliferations: surface marker phenotypes in clinical correlations.J Clin Oncol 1, 180 (1983).

    Google Scholar 

  30. Rudders R A, Poldre P A: The B cell IgM Fc receptor: further evidence for the B cell origin of ‘null’ chronic lymphocytic leukemia.Blood 64, 375 (1984).

    PubMed  CAS  Google Scholar 

  31. Haegert D, Coombs R: Do human B and null lymphocytes form a single immunoglobulin bearing population?Lancet ii, 1051 (1979).

    Article  Google Scholar 

  32. Gordon J: Molecular aspects of immunoglobulin expression by human B-cell leukemias and lymphomas.Adv Cancer Res 41, 72 (1984).

    Google Scholar 

  33. Knowles D, Halper J, Azzo W, Wang C: Reactivity of monoclonal antibodies LEU1 and OKT1 with malignant human lymphoid cells. Correlation with conventional cell markers.Cancer 52, 1369 (1983).

    Article  PubMed  Google Scholar 

  34. Mellstedt H, Holm G, Pettersson D, Peest D: Idiotype bearing lymphoid cells in plasma cell neoplasia, in Salmon S E (ed):Clinics in Hematology, vol. 11, p. 65. Philadelphia, W. B. Saunders (1982).

    Google Scholar 

  35. Herrmann F, Wirthmüller R: Cell surface marker phenotyping in patients with leukemic non-Hodgkin lymphomas (NHL) of low and intermediate malignancy.Immunobiology 163, 77 (1982).

    PubMed  CAS  Google Scholar 

  36. Conti I, Pacilli L, Zorli V, de Santes G, Manella E, Bornomo G, Laurenzi A, pandolfi F: Unsual phenotype (LEU7+, OKT4+, OKM1+) expressed by cells from patients with a normal expansion of granular lymphocytes.Acta Hemat 310, 315 (1984).

    Google Scholar 

  37. Ligler F S, Schlam M, Curley R, Brodski I, Bentzel J E: Monocyte markers and the common acute lymphoblastic leukemia antigen on chronic lymphocytic leukemic cells.Am J Hermat 15, 335 (1983).

    Article  CAS  Google Scholar 

  38. Gordon J, Mellstedt H, Åman P, Biberfeld P, Klein G: Phenotypic modulation of chronic lymphocytic leukemia cells by phorbol ester: induction of IgM secretion and changes in the expression of B cell associated surface antigens.J Immun 132, 541 (1984).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, Division of Hematology, Danderyd Hospital, S-104 01, Stockholm, Sweden

    E. Kimby & M. Björkholm

  2. Department of Immunology, Stockholm University, S-104 01, Stockholm, Sweden

    B. Axelsson

  3. Department of Clinical Immunology, Huddinge Hospital, S-104 01, Stockholm, Sweden

    G. Holm

  4. Radiumhemmet and Department of Medical Radiobiology, Karolinska Hospital, S-104 01, Stockholm, Sweden

    H. Mellstedt

  5. Department of Immunology, University of Birmingham, Birmingham, UK

    J. Gordon

Authors
  1. E. Kimby
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Axelsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Björkholm
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Gordon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Holm
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Mellstedt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kimby, E., Axelsson, B., Björkholm, M. et al. Characterization of chronic lymphocytic leukemia (CLL) cells with low density of surface membrane bound immunoglobulins (smIg). Med. Oncol. & Tumor Pharmacother. 2, 261–267 (1985). https://doi.org/10.1007/BF02934912

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02934912

Key words

Search

Navigation