Effect of prevention and treatment of murine acute viral myocarditis and protection of lymphoid organ atrophy with xinkang oral liquid

Abstract

Objective

The effect of prevention and treatment of Xinkang oral liquid, (

, XKOL) on experimental coxsackievirus B₃ (CVB₃) myocarditis mice model were investigated.

Methods

The mice were inoculated intraperitoneally with 0.3 ml of 10⁵ TCID₅₀ of CVB₃ to induce acute viral myocarditis model. These mice were divided into model control group (Group A), prevention high dosage group (Group B) and prevention low dosage group (Group C), treatment high dosage group (Group D) and treatment low dosage group (Group E), respectively. In addition, XKOL control group (Group F) and normal control group (Group G) were not infected with CVB₃ intraperitoneally. The administration of XKOL in Group B and C began 2 days before virus infection. All animals were sacrificed on day 20 for evaluation.

Results

Histological examination showed extensive myocardial necrosis and cell infiltration in most of Group A mice, but necrosis and cell infiltration were less severe in Group B,C,D and E mice. Thymus weight in Group B,C,D and E mice were heavier and less cell depletion occurred than those in Group A.

Conclussion

The XKOL could effectively inhibit myocardial CVB₃ replication, reduce the myocardial inflammatory response, lower incidence rate of myocarditis and prevent the disease associated lymphoid organ atrophy in this animal models.