Advertisement

Biologia Plantarum

, Volume 13, Issue 3, pp 200–206 | Cite as

Morphogenic studies in tissue cultures of the parasiteSantalum album L.

  • P. S. Rao
  • N. S. Rangaswamy
Article

Abstract

Whole seeds, excised embryos, and excised endosperm ofSantalum album were aseptically cultured with a view to studying seed germination in isolation from the host species, and to establishing callus cultures from both embryo and endosperm for comparative studies et their morphogenesis. Seed germination and seedling formation occurred normally only on modified White's medium supplemented with casein hydrolysate or coconut milk, or with both substances. Neither the excised embryo nor the endosperm grew on any of the culture media tested. However in about 17 per cent seed cultures on White's medium supplemented with 2,4-D, kinetin, and yeast extract, the endosperm degenerated, whereas the embryo callused and subsequently differentiated into innumerable embryoids; eventually the embryoids developed into normal plantlets. Callusing of the endosperm occurred also in seed cultures on four media supplemented variously with 2,4-D, kinetin, and yeast extract. Although the endosperm tissue grew through several passages no organ fornation was observed.

Keywords

Kinetin Seed Culture Callus Tissue Coconut Milk Santalum Album 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Morfogenetické studie explantátových kultur parasitické rostlinySantalum album L.

Abstract

Celistvá semena, extirpovaná embrya a extirpovaný endosperm rostlinSantalum album byly kultivovány za aseptických podmínek. Účelem práce bylo studovat klíčení semen v nepřítomnosti hostitelské rostliny a získat kalusové kultury z embryí i endospermu k srovnávacím morfogenetickým pokusům. Klíčení semen a utváření klíčních rostlin probíhalo normálně pouze na Whiteově mediu, k němuž byl dodán kasein-hydrolysát nebo kokosové mléko, anebo obě tyto látky. Extirpovaná embrya ani endosperm nerostly na žádném z testovaných kultivačních medií. V 17 % semen kultivovaných na Whiteově mediu, doplněném 2,4-D, kinetinem a kvasničným extraktem, docházelo k degeneraci endospermu, kdežto z embrya se vytvořil kalus, který postupně diferencoval ve velmi četné embryoidy; z embryoidů se nakonec vyvinuly normální rostlinky. K tvorbě kalusů z endospermu došlo také v semenech kultivovaných na čtyřoch půdách, k nimž byly přidány 2,4-D, kinetin a kvasničný extrakt v různých kombinacích. Ačkoliv pletivo z endospermu rostlo po několik pasáží, nebyla pozorována tvorba orgánů.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bhojwani, S. S.: Differentiation of haustoria in the germinating embryos of mistletoe without host stimulus.—Experientia25: 543–544, 1969.CrossRefPubMedGoogle Scholar
  2. Fineran, B. A.: Studies on the root parasitism ofExocarpus bidwillii Hook. f. VI. Haustorial attachment to non-living objects and the phenomenon of self-parasitism.—Phytomorphology15: 387–399, 1965.Google Scholar
  3. Johnson, F.: Transmission of plant viruses by dodder.—Phytopathology31: 649–656, 1941.Google Scholar
  4. Johri, B. M.: Chemical induction of polyembryony.—In:Ramakrishnan, C. V. (ed.): Tissue Culture.—Dr. W. Junk N. V. Publishers. The Hague, pp. 330–337, 1965.Google Scholar
  5. Johri, B. M., Bajaj, Y. P. S.: In vitro response of the embryo ofDendrophthoe falcata (L. f.)Ettings.—In:Maheshwari, P., Rangaswamy, N. S. (ed.): Plant Tissue and Organ Culture —A Symposium, Intern. Soc. Pl. Morphologists, Delhi, pp. 292–301, 1963.Google Scholar
  6. Johri, B. M., Babaj, Y. P. S.: Growth responses of globular proembryos ofDendrophthoe falcata (L. f.)Ettings in culture—Phytomorphology15: 292–300, 1965.Google Scholar
  7. Johri, B. M., Nag, K. K.: Experimental induction of triploid shoots in vitro from endosperm ofDendrophthoe falcata (L. f.)Ettings.—Curr. Sci.37: 606–607, 1968.Google Scholar
  8. Kristensen, H. R.: The sandal spike disease.—FAO, ETAP Report to Government of India, No. 1229: 1–31, 1960.Google Scholar
  9. Maheshwari, P., Baldev, B.: In vitro induction of adventive buds from embryos ofCuscuta reflexa Roxb.—In: Plant Embryology—A Symposium, C.S.I.R., New Delhi, pp. 129–138, 1962.Google Scholar
  10. Maheshwari, P., Rangaswamy, N. S.: Embryology in relation to physiology and genetics.— In:Preston, R. D. (ed.): Adv. bot. Res.2: 219–321, 1965.Google Scholar
  11. Petrů, E.: Development of embryoids in carrot root calllus culture (Daucus carota L.).—Biol. Plant.12: 1–5, 1970.CrossRefGoogle Scholar
  12. Raghavan, V., Torrey, J. G.: Inorganic nitrogen nutrition of the seedlings of the orchidCattleya.—Amer. J. Bot.51: 264–274, 1964.CrossRefGoogle Scholar
  13. Rangan, T. S., Rangaswamy, N. S.: Morphogenic investigations on parasitic angiosperms. I.Cistanche tubulosa (Orobanchaceae).—Can. J. Bot.46: 263–266, 1968.CrossRefGoogle Scholar
  14. Rangan, T. S., Rangaswamy, N. S.: Morphogenic investigations in parasitic engiosperms. III.Cassytha filiformis (Lauraceae).—Phytomorphology19: 292–300, 1969.Google Scholar
  15. Rangaswamy, N. S.: Experimental studies on female reproductive structures ofCitrus microcarpa Bunge.—Phytomorphology11: 109–127, 1961.Google Scholar
  16. Rangaswamy, N. S.: Studies on culturing seeds ofOrobanche aegyptiaca Pers.—In:Maheshwari, P., Rangaswamy, N. S. (ed.): Plant Tissue and Organ Culture—A Symposium Intern. Soc. Pl. Morphologists, Delhi, pp. 345–354, 1963.Google Scholar
  17. Rangaswamy, N. S.: Morphogenesis of seed germination in angiosperms.—Phytomorphology (Maheshwari Memorial Vol.)17: 477–487, 1967.Google Scholar
  18. Rangaswamy, N. S., Rao, P. S.: Experimental studies inSantalum album.—establishment of tissue culture of endosperm.—Phytomorphology13: 450–454, 1963.Google Scholar
  19. Rao, L. N.: Parasitism in theSantalaceae.—Ann. Bot. (N.S.)6: 131–150, 1942.Google Scholar
  20. Rao, M. R.: Germination and growth of sandal seedlings.—Ind. For. Rec.2: 137–157, 1910.15: 175–179, 1965.Google Scholar
  21. Srimathi, R. A., Sreenivasaya, M. S.: Studies on the culture of root tips, haustoria and lateral buds ofSantalum album L.—In:Maheshwari, P., Rangaswamy, N. S. (ed.): Plant Tissue and Organ Culture—A Symposium, Intern. Soc. Pl. Morphologists, Delhi, pp. 55–57, 1963.Google Scholar
  22. Sussex, I. M., Frei, K. A.: Embryoid development in long-term tissue cultures of carrot.— Phytomorphology18: 339–349, 1968.Google Scholar
  23. Truscott, F. H.: Some aspects of morphogenesis inCuscuta gronovii.—Amer. J. Bot.53: 739–750, 1966.CrossRefGoogle Scholar
  24. Varma, A., Chenulu, V. V., Raychaudhuri, S. P., Prakash, N., Rao, P.: Mycoplasma-like bodies in tissues infected with sandal spike and brinjal little leaf.—Ind. Phytopath.22: 289–291, 1969.Google Scholar
  25. Ziebur, N. K., Brink, R. A.: The stimulative effect ofHordeum endosperms on the growth of immature plant embryosin vitro.—Amer. J. Bot.38: 253–256, 1951.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany 1971

Authors and Affiliations

  • P. S. Rao
    • 1
  • N. S. Rangaswamy
    • 1
  1. 1.Department of BotanyUniversity of DelhiDelhi-7India

Personalised recommendations