Biotechnology and Bioprocess Engineering

, Volume 9, Issue 6, pp 440–446 | Cite as

Bipolar heterothallism, a principal mating system ofCordyceps militaris in vitro

  • Bhushan Shrestha
  • Ho-Kyung Kim
  • Gi-Ho Sung
  • Joseph W. Spatafora
  • Jae-Mo Sung


Interest inin vitro study of entomopathogenic fungi, includingCordyceps species, has been increasing due to their valuable bioactive compounds and biocontrol effects. AmongCordyceps species,in vitro stromata ofC. militaris has been successfully produced and cultivated for industrial purposes. However, genetic study onin vitro stromata formation ofC. militaris has not been carried out yet. Here, relationship between mating system and perithecial stromata formation ofC. militaris is reported. Mating system was determined by observing perithecial stromata formation from mono-ascospore cultures and their pair-wise combinations. Certain combinations of mono-ascospore strains produced perithecial club-shaped stromata, whereas other combinations produced either no stromata or only abnormal non-perithecial stromata. Similarly, monoascospore cultures without combination produced either no stromata or only abnormal nonperithecial stromata. Despite obvious heterothallism, self-fertility was occasionally observed in few strains ofC. militaris. These observations indicated thatC. militaris behaves as a bipolar heterothallic fungus and requires two mating compatible strains in order to produce regular clubshaped perithecial stromata, a fundamental requirement for its industrial cultivation.


artificial fruiting bipolar heterothallism hermaphroditic nature mating system selffertility 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2004

Authors and Affiliations

  • Bhushan Shrestha
    • 1
  • Ho-Kyung Kim
    • 2
  • Gi-Ho Sung
    • 3
  • Joseph W. Spatafora
    • 3
  • Jae-Mo Sung
    • 1
  1. 1.Department of Applied BiologyKangwon National UniversityChuncheonKorea
  2. 2.Mushtech Co. LtdChuncheonKorea
  3. 3.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA

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