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Optimization for a recombinantE. coli fed-batch fermentation

  • Session 3 Bioprocessing Research
  • Published:
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Abstract

The operating strategy that produces the maximum foreign protein expression for a fed-batch process is desired. This is achieved by using a feasible quadratic programming (FSQP) algorithm with a structured model that describes cell growth and product formation for recombinantE. coli. Optimization calculations for a fed-batch culture have not been performed with a model of this complexity up to this point. A constraint on the maximum cell concentration was included. For a fixed value of batch time, the results show that the optimal time profile of feed flow rate can increase the yield of foreign protein by 12–29% over a constant feed rate policy. Also, it was found that the computation time for the FSQP algorithm can be reduced significantly by considering suboptimal profiles of the feed rate, with a minor effect on calculated protein yield.

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Author notes

  1. Q. Chen

    Present address: Cray Research, 55121-1500, Eagan, MN

Authors and Affiliations

  1. Department of Chemical Engineering, University of Maryland, 20742, College Park, MD

    Q. Chen, W. E. Bentley & W. A. Weigand

  2. Center for Agricultural Biotechnology, University of Maryland, 20742, College Park, MD

    W. E. Bentley

Authors
  1. Q. Chen
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  2. W. E. Bentley
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  3. W. A. Weigand
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Chen, Q., Bentley, W.E. & Weigand, W.A. Optimization for a recombinantE. coli fed-batch fermentation. Appl Biochem Biotechnol 51, 449–461 (1995). https://doi.org/10.1007/BF02933447

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  • DOI: https://doi.org/10.1007/BF02933447

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