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Fermentation of sugars in organe peel hydrolysates to ethanol by recombinantEscherichia coli KO11

  • Session 3 Bioprocessing Research
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Abstract

The conversion of monosaccharides in organe peel hydrolysates to ethanol by recombinantEscherichia coli KO11 has been investigated in pH-controlled batch fermentations at 32 and 37°C. pH values and concentration of peel hydrolysate were varied to determine approximate optimal conditions and limitations of these fermentations. Very high yields of ethanol were achieved by this microorganism at reasonable ethanol concentrations (28–48 g/L). The pH range between 5.8 and 6.2 appears to be optimal. The microorganism can convert all major monosaccharides in organe peel hydrolysates to ethanol and to smaller amounts of acetic and lactic acids. Acetic acid is coproduced in equimolar amounts with ethanol by catabolism of salts of galacturonic acid.

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Authors and Affiliations

  1. US Citrus and Subtropical Products Laboratory, 600 Avenue S, N.W., P.O. Box 1909, 33883-1909, Winter Haven, Florida

    K. Grohmann & R. G. Cameron

  2. Florida Department of Citrus, 600 Avenue S, N.W., P.O. Box 1909, 33883-1909, Winter Haven, Florida

    B. S. Buslig

Authors
  1. K. Grohmann
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  2. R. G. Cameron
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  3. B. S. Buslig
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South Atlantic Area, Agricultural Research Service, US Department of Agriculture. Mention of a trademark or proprietary product is for identification only, and does not imply a guarantee or warranty of the product by the US Department of Agriculture. All programs and services of the US Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap.

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Grohmann, K., Cameron, R.G. & Buslig, B.S. Fermentation of sugars in organe peel hydrolysates to ethanol by recombinantEscherichia coli KO11. Appl Biochem Biotechnol 51, 423–435 (1995). https://doi.org/10.1007/BF02933445

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