Folia Microbiologica

, 37:210 | Cite as

Symbiotic effectiveness of Hup+ Rhizobium, VAM fungi and phosphorus levels in relation to nitrogen fixation and plant growth ofCajanus cajan

  • G. K. Sekhon
  • R. P. Gupta
  • M. S. Pandher
  • J. K. Arora


Effect of hydrogen uptake positive (Hup+) strain ofRhizobium sp. (pigeon pea) and VAM fungus (Glomus fasciculatum) was studied on the symbiotic parameters of pigeon pea (Cajanus cajan) cv. AL-15 at various levels of phosphorus. The Hup+ Rhizobium strain showed more nodulation, plant biomass and plant nitrogen content than its Hup counterpart. VAM infection in pigeon pea roots helped in translocating phosphorus from the soil and improved nitrogen fixation. Similarly, addition of phosphorus was found to play a positive role in enhancing all these parameters. Dual inoculation of Hup+ Rhizobium strain and VAM significantly increased nodulation, nitrogenase activity, plant nitrogen and phosphorus content and plant biomass compared to single inoculation of either organism and dual inoculation with Hup and VAM fungus.


Nitrogen Fixation Rhizobium Strain Hydrogen Uptake Dual Inoculation Phosphorus Application 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Behki R.M., Selvaraj G., Iyer V.N.: Derivatives ofRhizobium meliloti strains carrying a plasmid ofR. leguminosarum specifying hydrogen uptake and peaspecific symbiotic functions.Arch.Microbiol. 140, 352–357 (1985).CrossRefGoogle Scholar
  2. Cunningham S.D., Kapaiaik Y., Brewin N.J., Phillips D.A.: Uptake hydrogenase activity determined by plasmid pRL6J1 inR. leguminosarum does not increase symbiotic nitrogen fixation.Appl.Environ.Microbiol. 50, 791–794 (1985).PubMedGoogle Scholar
  3. Dejong T.M., Brewin N.J., Johnston A.W.B., Phillips D.A.: Improvement of symbiotic properties inR. leguminosarum by plasmid transfer.J.Gen.Mcrobiol. 128, 1829–1838 (1982).Google Scholar
  4. Drevon J.J., Frazier L., Russell S.A., Evans H.J.: Respiratory and nitrogenase activities of soybean nodules formed by hydrogen uptake negative (Hup) mutant and revertant strains ofR. japonicum characterized by protein patterns.Plant Physiol. 70, 1341–1346 (1982).PubMedGoogle Scholar
  5. Hanus F.J., Albrecht S.L., Zablotowicz R.M., Emerich D.W., Russell S.A., Evans H.J.: The effect of the hydrogenase system inRhizobium japonicum inocula on the nitrogen content and yield of soybean seed in field experiments.Agron.J. 73, 368–372 (1981).Google Scholar
  6. Hanus F.J., Carter K.R., Evans H.J.: Techniques for measurement of hydrogen evolution by nodules.Methods Enzymol. 69, 731–739 (1980).CrossRefGoogle Scholar
  7. Hanus F.J., Maier R.J., Evans H.J.: Autotrophic growth of H2uptake positive strains ofR. japonicum in an atmosphere supplied with hydrogen gas.Proc.Nat.Acad.Sci.USA 76, 1788–1792 (1979).PubMedCrossRefGoogle Scholar
  8. Hardy R.W.F., Burns R.C., Holsten R.D.: Application of acetyleneethylene assay for measurement of nitrogen fixation.Soil Biol.Biochem. 5, 47–81 (1973).CrossRefGoogle Scholar
  9. Jackson M.L.:Soil Chemical Analysis. Prentice Hall, New Delhi (India) 1973.Google Scholar
  10. Maier R.J., Campbell N.E.R., Hanus F.J., Simpson F.B., Russell S.A., Evans H.J.: Expression of hydrogenase activity in free livingR. japonicum.Proc.Nat.Acad.Sci.USA 75, 3258–3262 (1978).PubMedCrossRefGoogle Scholar
  11. Manjunath A., Bagyaraj D.J.: Response of pigeonpea and cowpea to phosphate and dual inoculation with vesicular arbuscular mycorrhiza andRhizobium.Trop.Agric. 61, 48–52 (1984).Google Scholar
  12. McGraw A.C., Hendrix J.W.: Host and soil fumigation effects on spore population densities of species of endogonaceous mycorrhizal fungi.Mycologia 76, 122–131 (1984).CrossRefGoogle Scholar
  13. McKenzie H.A., Wallace H.A.: The Kjeldahl determination of nitrogen.Austral.J.Chem. 17, 55–79 (1954).Google Scholar
  14. Pahwa K. Dogra R.C.: H2-recycling system in mungbeanRhizobium in relation to N2 fixation.Arch.Microbiol. 121, 380–383 (1981).CrossRefGoogle Scholar
  15. Punj V., Gupta R.P.: Response of Subabul to inoculation with vesiculararbuscular mycorrhiza, phosphorus and nitrogen fertilizers.J.Res., Punjab Agric.Univ., Ludhiana (India)26, 450–456 (1989).Google Scholar
  16. Rainbird R.M., Atkins C.A., Pate J., Sanford P.: Significance of hydrogen evolution in the carbon and nitrogen economy of nodulated cowpea.Plant Physiol. 71, 122–127 (1983).PubMedCrossRefGoogle Scholar
  17. Rena J.I., Sanchez Diaz M., Aguirreoleo J., Becana M.: Increased stress tolerance of nodule activity in theMedicago — Rhizobium — Glomus symbiosis under drought.J.Plant Physiol. 133, 79–83 (1988).Google Scholar
  18. Sekhon G.K.: Legume —Rhizobium symbiosis for enhanced nitrogen fixation in pigeonpea.MSc Thesis. Punjab Agricultural University, Ludhiana (India) 1990.Google Scholar
  19. Subba Rao N.S., Tilak K.V.B.R., Singh C.S.: Dual inoculation withRhizobium sp. andGlomus fasciculatum enhances nodulation, yield and nitrogen fixation in chickpea (Cicer arietinum Linn.).Plant & Soil 95, 351–359 (1986).CrossRefGoogle Scholar
  20. Suman Bala, Singh O.S.: Response of lentil to VA mycorrhizal inoculation and plant available P levels of unsterile soils.Plant & Soil 87, 445–447 (1985).CrossRefGoogle Scholar
  21. Vincent J.M.:A Manual for the Practical Study of Root Nodule Bacteria. Blackwell Scientific Publications, Oxford 1970.Google Scholar
  22. Wilson D.O., Reisenauer H.M.: Determination of leghemoglobin in legume nodules.Anal.Biochem. 6, 27–30 (1963).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1992

Authors and Affiliations

  • G. K. Sekhon
    • 1
  • R. P. Gupta
    • 1
  • M. S. Pandher
    • 1
  • J. K. Arora
    • 1
  1. 1.Department of MicrobiologyPunjab Agricultural UniversityLudhiana - 141 004, PunjabIndia

Personalised recommendations