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Partial purification and characterization of an extracellular protease fromXenorhabdus nematophilus, a symbiotic bacterium isolated from an entomopathogenic nematode,Steinernema glaseri

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Abstract

Entomopathogenic nematodes are used for insect control. Herein, an extracellular protease was partially purified from a culture supernatant ofXenorhabdus nematophilus, a symbiotic bacterium of an entomopathogenic nematode,Steinernema glaseri, using precipitation with 80% v/v isopropyl alcohol followed by gel permeation chromatography with a packed Sephacryl S-300 HR media. The partially purified protease exhibited maximal activity at pH 7 in the presence of 1 mM CaCl2. The protease was identified as a metallo-protease based on the inhibition of its activity by the metal chelating agent, EDTA.

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Correspondence to Keun-Garp Ryu.

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Chae, YR., Ryu, KG. Partial purification and characterization of an extracellular protease fromXenorhabdus nematophilus, a symbiotic bacterium isolated from an entomopathogenic nematode,Steinernema glaseri . Biotechnol Bioproc E 9, 379–382 (2004). https://doi.org/10.1007/BF02933061

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  • DOI: https://doi.org/10.1007/BF02933061

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