Biologia Plantarum

, Volume 14, Issue 6, pp 385–389 | Cite as

Conditions for the extraction of DNA and RNA from tobacco pollen by sodium chloride and perchloric acid

  • J. Süss


Studying the influence of the pH of 10% NaCl solutions used for the extraction of RNA and DNA on the yield of both nucleic acids, the maxima of pH were found at which both types of nucleic acids pass into extracts better than at neutral pH and do not remain in residues of the experimental material.The perchloric acid extraction temperature was also studied for obtaining the hydrolysate of nucleic acids from the trichloroacetic acid precipitate of sodium chloride extracts differing by 5 °C within the range of 35 °C to 90 °C and it was found that in this wide range almost the same amount of RNA is extracted by the method used. However, at a lower temperature, some DNA remained in the extracted residue of the trichloroacetic acid precipitate of sodium chloride extracts.


Nucleic Acid Diphenylamine Orcinol Tobacco Pollen Washed Material 



trichloroaoetic acid


perchloric acid


nucleic acids


phosphorus of the sum of nucleic acids


phosphorus of ribonucleic acid


phosphorus of deoxyribonueleio acid






Lowry positive substances


residue of the TCA precipitate after PCA extraction


residue of the pollen matter after NaCl extraction


original fraction from pollen

Podminky extrakce DNA a RNA z tabákového pylu pomocí roztoků chloridu sodného a kyseliny chloristé


Pŕi studiu vlivu pH roztoku 10% chloridu sodného užitého k extrakci RNK a DNK na vítěžek obou typů nukleovích kyselin byla nalezena maxima pH, pŕi nichž oba typy nukleovích kyselin lépe pŕecházejí do extraktu než pŕi neutrálním pH a nezůstávají ve zbytku pokusného materiálu po extrakci. Studovány byly také extrakční teploty pro získaní hydrolysátu nukleovích kyselin kyselinou chloristou ze sraženiny získane kyselinou trichloroctovou z extraktu v roztoku chloridu sodného, lišící se o 5 °C v rozmezí 35 °C až 90 °C. Bylo zjiatěno, že v tomto širokém rozmezí teplot se použitou metodou vyextrahuje pŕibližně stejně velkě množství RNK. Avšak pŕi nižŮích teplotách část DNK zůstává v extrahovaném zbytku sraženiny získané kyselinou trichloroctovou z extraktů v roztoku chloridu sodného.


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Copyright information

© Institute of Experimental Botany 1972

Authors and Affiliations

  • J. Süss
    • 1
  1. 1.Department of Genetics, Institute of Experimental BotanyCzechoslovak Academy of SciencesPraha 6 — DejviceCzechoslovakia

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