Virchows Archiv B

, Volume 40, Issue 1, pp 181–189 | Cite as

An immunohistochemical study of cells with surface and cytoplasmic immunoglobulins in situ in Peyer’s patches and lamina propria of rat small intestine

  • T. Sminia
  • Benita E. C. Plesch


The distribution of cells with surface and cytoplasmic immunoglobulins was studied in Peyer’s patches (PP) and intestine of rats, using both frozen and paraffin sections, with a two-step peroxidase technique. And IgM, IgG, IgA and IgE sera were used. Surface staining was found within PP with all antisera used. Although the villi contained predominantly IgA plasma cells (PC), IgG PC and a few IgM and IgE PC were also found. Within PP, however, no IgA PC were found but IgM and IgG PC were present in all stages of development, mainly in the dome. PC of all types, but mostly IgA cells, were present in and around high endothelial venules (HEV). The results suggest that IgM and IgG PC precursors can develop to PC within PP whereas IgA precursors do not. PC appear to home to the gut preferentially via HEV.

Key words

Peyer’s patches Rat Immunohistochemistry Plasma cells Peroxidase technique 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • T. Sminia
    • 1
  • Benita E. C. Plesch
    • 1
  1. 1.Department of Histology, Medical FacultyFree University AmsterdamMC AmsterdamThe Netherlands

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