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Virchows Archiv B

, Volume 40, Issue 1, pp 27–37 | Cite as

Estrogen receptors in human breast cancer

II. Correlation between the histochemical method and biochemical assay
  • C. J. L. M. Meijer
  • J. van Marle
  • J. P. Persijn
  • W. van Niewenhuizen
  • J. P. A. Baak
  • M. E. Boon
  • J. Lindeman
Article

Summary

The present study defines criteria for determining the presence of estrogen-receptors in human breast carcinomas demonstrated by a histochemical assay using 17β-estradiol-carboxy-methyl-oxim-bovine serum albumen-FITC.

The criteria were: 1) the percentage of cells showing fluorescence; 2) the intensity of the fluorescence observed, and 3) the percentage of epithelial structures in tissue specimens. Using these predefined criteria in 132 human breast carcinomas a 91.6% agreement was found between the results of the histochemical assay and those of the biochemical Charcoal method. The main causes of disagreement (7 of the 11 cases) were sampling errors between the tissue specimens used for the histochemical and biochemical assay, and an insufficient percentage of epithelial structures (< 15%) to allow biochemical identification of estrogen receptor activity. In the hands of pathologists with experience in the field of histochemistry this histochemical assay may be the method of choice for the assessment of estrogen receptors.

Key words

Breast carcinoma Estrogen receptors Immunohistochemistry Biochemistry 

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References

  1. Baak JPA, Agrafojo Blanco A, Kurver PHJ, Langley FA, Boon ME, Lindeman J, Overdiep SH, Niewlaat A, Brekelmans E (1981) Quantitation of borderline and malignant mucinous ovarian tumors. Histopathology 5:353–360PubMedCrossRefGoogle Scholar
  2. Chamnes GC, Mercer WD, McGuire WL (1980) Are histochemical methods for estrogen receptor valid? J Histochem Cytochem 28:792–798Google Scholar
  3. Heuson JC, Longeval E, Matheiem WH, Deboel MC, Sylvester RJ, Leclerq G (1972) Significance of quantitative assessment of estrogen receptors for endocrine therapy in advanced breast cancer. Cancer 39:1971–1977CrossRefGoogle Scholar
  4. Korsten CB, Persijn JP (1977) Evaluation of, and additional data of an improved simple charcoal method to determine estrogen receptors. J Clin Chem Biochem 15:297–301Google Scholar
  5. Lee SH (1980) Cellular estrogen and progesterone receptors in mammary carcinoma. Am J Clin Pathol 73:323–329PubMedGoogle Scholar
  6. Lee SH (1981) The histochemistry of estrogen receptors. Histochemistry 71:491–500PubMedCrossRefGoogle Scholar
  7. McCarthy KS, Woodard BH, Nichols DE, Wilkinson W, McCarthy KSSR (1980) Comparison of biochemical and histochemical techniques for estrogen receptor analysis in mammary carcinoma. Cancer 46:2842–2845CrossRefGoogle Scholar
  8. McGuire WL, Horwitz KB, Zava DT, Carola RE, Chamnes GC (1978) Hormones in breast cancer update 1978. Metabolism 27:487–501PubMedCrossRefGoogle Scholar
  9. Meijer JS, Stevens SC, White WL, Hixon B (1978) Estrogen receptor assay of carcinoma of the breast by a simplified dextrancharcoal method. Am J Clin Pathol 70:655–659Google Scholar
  10. Pertschuck LP, Tobin EH, Brigati DJ, Kim DS, Bloom ND, Gaetjens E, Berman PJ, Carter AC, Degen Shein GA (1978) Immunofluorescent detection of estrogen receptors in breast cancer. Cancer 41:907–911CrossRefGoogle Scholar
  11. Pertschuck LP, Tobin EH, Tanapat P, Gaetjens E, Carter AC, Bloom ND, Macchia RJ, Eisenberg KB (1980) Histochemical analysis of steroid hormone receptors in breast and prostatic carcinoma. J Histochem Cytochem 28:799–810Google Scholar
  12. Stallmann PJ, Wagenaar SjSc, Swierenga J, Van der Wal J, Feltkamp-Vroom ThM (1977) Cellbound IgE on tumor mast cells and basophylic granulocytes in atopic and non-atopic subjects. Int Arch All Appl Immunol 54:443–450Google Scholar
  13. Taylor CR, Cooper CL, Kurman RJ, Goebelsmann U, Markland FS (1981) Detection of estrogen receptor in breast and endometrial carcinoma by the immunoperoxidase technique. Cancer 47:2634–2640PubMedCrossRefGoogle Scholar
  14. Van Marie J, Lindeman J, Ariëns AT, Labruyère W, Van Weeren-Kramer J (1982) Estrogen receptors in human breast cancer. I. Specificity of the histochemical localization of estrogen receptors using an estrogen-albumin F.I.T.C. complex. Virchows Arch [Cell Pathol] 39:17–25Google Scholar
  15. Walker RA, Cove DH, Howell A (1980) Histological detection of estrogen receptor in human breast carcinomas. Lancet I:171–173CrossRefGoogle Scholar
  16. Walker RA (1981) The cytochemical demonstration of estrogen receptors in human breast carcinomas. J Pathol 135:237–247PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • C. J. L. M. Meijer
    • 1
  • J. van Marle
    • 2
  • J. P. Persijn
    • 4
  • W. van Niewenhuizen
    • 3
  • J. P. A. Baak
    • 1
  • M. E. Boon
    • 1
  • J. Lindeman
    • 1
  1. 1.Department of PathologyS.S.D.Z.AD DelftThe Netherlands
  2. 2.Pharmacological LaboratoryUniversity of AmsterdamThe Netherlands
  3. 3.Gaubius InstituteLeidenThe Netherlands
  4. 4.Antoni van Leeuwenhock HospitalAmsterdam

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