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Enhancement ofcis,cis-muconate productivity by overexpression of catechol 1,2-dioxygenase inPseudomonas putida BCM114

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Abstract

For enhancement ofcis,cis-muconate productivity from benzoate, catechol 1,2-dioxygenase (C12O) which catalyzes the rate-limiting step (catechol conversion tocis,cis-muconate) was cloned and expressed in recombinantPseudomonas putida BCM114. At higher benzoate concentrations (more than 15 mM),cis,cis-muconate productivity gradually decreased and unconverted catechol was accumulated up to 10 mM in the case of wildtypeP. putida BM014, whereascis,cis-muconate productivity continuously increased and catechol was completely transformed tocis,cis-muconate forP. putida BCM114. Specific C12O activity ofP. putida BCM114 was about three times higher than that ofP. putida BM014, and productivity was enhanced more than two times.

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Authors and Affiliations

  1. Department of Chemical Technology, College of Engineering, Seoul National University, 151-742, Seoul, Korea

    Beum Jun Kim, Won Jae Choi & Cha Yong Choi

  2. Department of Food Science and Technology, College of Engineering, Kyungsung University, 608-736, Pusan, Korea

    Eun Yeol Lee

Authors
  1. Beum Jun Kim
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  2. Won Jae Choi
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  3. Eun Yeol Lee
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  4. Cha Yong Choi
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Correspondence to Cha Yong Choi.

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Kim, B.J., Choi, W.J., Lee, E.Y. et al. Enhancement ofcis,cis-muconate productivity by overexpression of catechol 1,2-dioxygenase inPseudomonas putida BCM114. Biotechnol. Bioprocess Eng. 3, 112–114 (1998). https://doi.org/10.1007/BF02932513

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