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Elicitation of camptothecin production in cell cultures ofCamptotheca acuminata

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Abstract

Campothecin production was increased with elicitors, methyl jasmonate, jasmonic acid, yeast extract elicitor, and ferulic acid in suspension cultures ofCamptotheca acuminata. jasmonic acid was found to be the most efficient elicitor. Camptothecin production increased 11 times by using the optimum dosing concentration of jasmonic acid which was 50 μM. The kinetics of camptothecin accumulation in response to the treatment with jasmonic acid showed that the camptothecin accumulation reached the maximum value at 4 days after jasmonic acid dosing and then a rapid decrease in camptothecin accumulation was observed.

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Authors and Affiliations

  1. School of Chemical Engineering and Biotechnology, Ajou University, San 5, Wonchon-dong, Paldal-ku, 442-749, Suwon-city, Kyungki-do, Korea

    Sang Yo Byun

  2. Department of Chemical Engineering, Seoul National University, Seoul, Korea

    Seung Hoon Song

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  1. Seung Hoon Song
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  2. Sang Yo Byun
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Correspondence to Sang Yo Byun.

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Song, S.H., Byun, S.Y. Elicitation of camptothecin production in cell cultures ofCamptotheca acuminata . Biotechnol. Bioprocess Eng. 3, 91–95 (1998). https://doi.org/10.1007/BF02932509

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