Abstract
It has been proved that co-cultivation of human neuroblastoma cells and human fibrolast cells can enhance nerve cell growth and the production of BDNF in perfusion cultivation. In batch co-cultivation, maximum cell density was increased up to 1.76×106 viable cells/mL from 9×105 viable cells/mL of only neuroblastoma cell culture. The growth of neuroblastoma cells was greatly improved by culturing both nerve and fibroblast cells in a perfusion process, maintaining 1.5×106 viable cells/mL, which was much higher than that from fed-batch cultivation. The nerve cell growth was greatly enhanced in both fed-batch and perfusion cultivations while the growth of fibroblast cells was not. It strongly implies that the factors secreted from, human fibroblast cells and/or the environments of co-culture system can enhance both cell growth and BDNF secretion. Specific BDNF production rate was not enhanced in co-cultures; however, the production period was increased as the cell growth was lengthened in the co-culture case. Competitive growth between nerve cells and fibroblast cells was not observed in all cases, showing no changes of fibroblast cell growth and only enhancement of the neuroblastoma cell growth and overall BDNF production. It was also found that the perfusion cultivation was the most appropriate process for cultivating two cell lines simultaneously in a bioreactor.
Similar content being viewed by others
References
Roush, W. (1996) New neurons use outlook to navigate nervous system.Science 271:1807–1808.
Silos, S. I. and W. S. Snider (1992) Development of commissural neurons in the embryonic rat spinal cord.J. Com. Neurol. 325: 514–526.
Kumar, S., J. Huber, L. A. Pena, and J. de Vallis (1990) Characterization of functional nerve growth factor-receptor in a CNS glial cell line.J. Neurosci. Res. 27:408:417.
Merlino, J. P., P. Ernfors, Z. Kokaiza, D. S. Middlemas, and H. Persson (1993) Increase production of the trkB protein kinase receptor after brain insults.Neuron. 10:151–164.
Greene, L. A. and A. S. Tischler (1976) Establishment of a noradrenergic clonal line of rat pheochromocytoma cells which respond to nerve growth factor.Proc. Natl. Acad. Sci. USA 73: 2424–2428.
Barigana, M. (1994) Neurotrophic factors enter the clinic.Science 264:772–774.
Schorderet, M. (1995) Alzheimer’s disease: fundament and therapeutic aspects.Experientia 51:99–105.
Frank, E. and P. Westerfield (1983) Development of sensory-motor synapes in the spinal cord of the frog.J. Physiol. 343:593–610.
Wang, A. A., G.K. Ojakian and W. J. Nelson (1990) Steps in the morphogenesis of a polarized epithelium.J. Cell Sci. 95:137–151.
Spier, R. E., J. R. Griffiths and Berthold, W. eds. (1994)Animal cell culture technology, ESACT, Butterworth Publ., London.
Paine, A. J., L. Williams and L. J. Hockins (1989) Potent maintenance by metyrapone.Biochem. J. 188:937–942.
Freshney, R. I. (ed.) (1983)Cultivation of animal cells. Alan R. Liss, Inc. pp. 199, New NY.
Hong, J. S., K. H. Woo, K. Y. Park and H. Y. Lee (1997) The Secretion of BDNF from the cultures of human neuroblastoma cells.Kor. J. Microbiol. Biotechnol. 25:102–105.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hong, JS., Oh, SJ., Kim, SH. et al. Enhancement of BDNF production by co-cultivation of human neuroblastoma and fibroblast cells. Biotechnol. Bioprocess Eng. 3, 51–54 (1998). https://doi.org/10.1007/BF02932501
Issue Date:
DOI: https://doi.org/10.1007/BF02932501