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Biochemical analysis of BmNPV attachment toBombyx mori BmN-4 cells

  • Tae Yong Kim
  • Han Chul Kang
  • Jin O Park
  • Jong Hwa Park
  • Seok Kwon Kang
  • In Sik Chung
Article
  • 34 Downloads

Abstract

Binding characteristics ofBombyx mori nuclear polyhedrosis virus (BmNPV) toB. mori BmN-4 cells was determined. The cells had a single class of BmNPV-binding sites and displayed a low binding capacity for BmNPV in the range of 70 sites per cell. The biochemical nature of BmNPV-binding sites on the cell surface was identified. Treatment of BmN-4 cells with protease, wheat germ agglutinin, metabolic inhibitors, or lysosomotropic agents reduced BmNPV binding to BmN-4 cells, whereas treatment with phospholipase C showed no effect on virus binding. These results indicate that the structure of the binding site moiety is a protein-oligosaccharide complex.

Key words

Bombyx mori nuclear polyhedrosis virus BmN-4 cells binding site 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering 1997

Authors and Affiliations

  • Tae Yong Kim
    • 1
  • Han Chul Kang
    • 2
  • Jin O Park
    • 1
  • Jong Hwa Park
    • 1
  • Seok Kwon Kang
    • 3
  • In Sik Chung
    • 1
  1. 1.Institute and Department of Genetic EngineeringKyung Hee UniversitySuwonKorea
  2. 2.Rural Development AdministrationSuwonKorea
  3. 3.Department of Agricultural BiologySeoul National UniversitySuwonKorea

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