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Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng

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Abstract

Among the various plant growth regulators (PGR), 9.0 μM 2,4-D(2,4-dichlorophenoxyacetic acid) turned out to be the best for callogenesis and 4.5 μM 2,4-D was revealed to be more suitable for callus proliferation. Of the four basal media, the highest number of 8.2 adventitious roots was induced in Woody plant medium (WPM) containing 14.8 μM IBA(indole 3-butyic acid). F tests on each auxin treatment in the ANOVA revealed highly significant difference at α=0.01. The number of multiple adventitious roots (MARs) formed in each adventitious roots highly depended on the types and levels of auxin tested. At both 24.6 μM and 34.4 μM IBA, more than 25 MARs were induced after 4 weeks of culture. When different medium were applied, immersion type showed the highest fresh weight of MARs at the time of harvest. With 160 g fresh weight of inoculum, about 14.2 times of biomass was obtained after 6 weeks of culture. Identical fractionation pattern of the amplified fragment length polymorphism (AFLP) analysis was observed between the adventitious root and donor of a mountain ginseng. This observation suggested that any rearrangements of the genomic DNA had not occurred in the adventitious roots after 1 year of cultures. The cultivated ginseng and the mountain ginseng groups could be distinguished by 2 AFLP markers.

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Authors and Affiliations

  1. Division of Biotechnology, Forestry Research Institute, Forestry Administration, 44-3 Omokcheon-dong, 441-350, Suwon, Korea

    Sung Ho Son, Sung Mee Choi, Soo Jin Hyung, Seung Rho Yun & Myung Suk Choi

  2. Division of Genetics and Physiology, Forestry Research Institute, Forestry Administration, 44-3 Omokcheondong, 441-350, Suwon, Korea

    Eun-Myoung Shin & Yong-Pyo Hong

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  1. Sung Ho Son
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  2. Sung Mee Choi
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Correspondence to Sung Ho Son.

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Son, S.H., Choi, S.M., Hyung, S.J. et al. Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng. Biotechnol. Bioprocess Eng. 4, 119–123 (1999). https://doi.org/10.1007/BF02932381

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