Skip to main content
SpringerLink
Log in

Characterization of sulfur oxidation by an autotrophic sulfur oxidizer,Thiobacillus sp. ASWW-2

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

An autotrophic sulfur oxidizer,Thiobacillus sp. ASWW-2, was isolated from activated sludge, and its sulfur oxidation activity was characterized.Thiobacillus sp. ASWW-2 could oxidize elemental sulfur on the broad range from pH 2 to 8. When 5–50 g/L of elemental sulfur was supplemented as a substrate, the growth and sulfur oxidation activity ofThiobacillus sp. ASWW-2 was not inhibited. The specific sulfur oxidation rate of strain ASWW-2 decreased gradually until sulfate was accumulated in medium up to 10 g/L. In the range of sulfate concentration from 10 g/L to 50 g/L, the sulfur oxidation rate could keep over 2.0 g-S/g-DCW-d. It indicated thatThiobacillus sp. ASWW-2 has tolerance to high concentration of sulfate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Eikum, A. S. and R. Storhang (1986) Odour problems related to waste water and sludge treatment. pp. 12–18. In: V. C. Neilsen, J. H. Voorgurg, and P. L. Hermite, (Eds.),Odour Provention and Control of Organic Sludge and Livestock Farming. Elsevier Applied Science Publishers, London.

    Google Scholar 

  2. Yong, Y. and E. R. Allen (1994) Biofiltration control of hydrogen sulfide 1. Design and operational parameters.J. Air Waste Magm. Assoc. 44: 863–868.

    Google Scholar 

  3. Vanhoorne, M., A. Rouck, and D. de. Bacquer (1995) Epidemiological study of eye irritation by hydrogen sulphide and or carbon disulphid exposure in viscose rayon workers.Ann. Occup. Hyg. 39: 307–315.

    CAS  Google Scholar 

  4. Mori, T., M. Koga, Y. Hikosaka, T. Nonaka, F. Mishina, Y. Sakai, and J. Koizumi (1991) Microbial corrosion of concrete sewer pipes, H2S production from sediments and determination of corrosion rate.Wat. Sci. Tech., 23: 1275–1282.

    CAS  Google Scholar 

  5. Cho, K. S. and T. Mori (1995) A newly isolated fungus participates in the corrosion of concrete sewer pipes.Wat. Sci. Tech. 31: 263–271.

    Article  CAS  Google Scholar 

  6. Shoda, M. (1991) Methods for the biological treatment of exhaust gases in biological degradation of waste. pp. 31–46. In: A. M. Martin (ed.),Biological Degradation of Wastes. Elsevier Applied Science Publishers, London.

    Google Scholar 

  7. Cho, K. S., L. Zhang, M. Hirai and M. Shoda (1991) Removal characteristics of hydrogen sulfide and methanethiol byThiobacillus sp. isolated from peat in biological deodorization.J Ferment. Bioeng. 71: 44–49.

    Article  CAS  Google Scholar 

  8. Rands M. B., D. E. Cooper, C. P. Woo, G. C. Fletcher, and K. A. Rolfe (1981) Compost filters for H2S removal from anaerobio digestion and rendering exhausts.J. Water Pollut. Control Fed. 53: 185–189.

    CAS  Google Scholar 

  9. Cho, K. S., M. Hirai and M. Shoda (1991) Degradation characteristics of hydrogen sulfide, methanethiol, dimethyl sulfide and dimethyl disulfide byThiobacillus thioparus DW44 isolated from peat biofilter.J. Ferment. Bioeng. 71: 384–339.

    Article  CAS  Google Scholar 

  10. Cho, K. S., M. Hirai and M. Shoda (1992) Enhanced removal efficiency of malodorous gases in a pilot-scale peat biofilter inoculate withThiobacillus thioparus DW44.J. Ferment. Bioeng. 73: 46–50.

    Article  CAS  Google Scholar 

  11. Sublette, K. L. and N. D. Sylvesten (1987) Oxidation of hydrogen sulfide byThiobacillus denitrificans: desulfurization of natural gas.Biotechnol Bioeng. 29: 249–257.

    Article  CAS  Google Scholar 

  12. Kanagawa, T. and E. Mikami (1989) Removal of methanethiol. dimethyl sulfide, dimethyl disulfide, and hydrogen sulfide from contaminated air byThiobacillus thioparus TK-m.Appl. Environ. Microbiol. 55: 555–558.

    CAS  Google Scholar 

  13. Chung, Y. C., C. Huang and C. F. Li (1997) Removal characteristics of H2S byThiobacillus novellus CH13 biofilter in autotrophic and mixotrophic environments.J. Environ. Sci. Health. A 32: 1435–1450.

    Article  Google Scholar 

  14. Larkin, J. M. (1980) Isolation ofThiotrix in pure culture and observation of a filamentous epiphyte onThiotrix.Curr. Microbiol. 4: 144–158.

    Article  Google Scholar 

  15. Nelson, D. C. and H. W. Jannasch (1983) Chemoautotrophic growth of a marineBeggiatoa in sulfide-gradient cultures.Arch. Microbiol. 136: 262–269.

    Article  CAS  Google Scholar 

  16. Zhang, L., M. Hirai and M. Shoda (1991) Removal characteristics of dimethyl sulfide methanethiol and hydrogen sulfide byHyphomicrobium sp. 155 isolated from peat biofilter.J. Ferment. Bioeng. 72: 392–396.

    Article  CAS  Google Scholar 

  17. Steinmetz, M. A. and U. Fisher (1982) Cytochromes of the green sulfur bacteriumChlorobium vibrioformef. thiosulfatophilum. Purification, characterization and sulfur metabolism.Arch. Microbiol. 131: 19–26.

    Article  CAS  Google Scholar 

  18. Gray, G. O. and J. G. Knaff (1982) The role of a cytochromec-552-cytochromec complex in the oxidation sulfide inChromatium vinosum.Biochim. Biophys. Acta 680: 290–296.

    Article  CAS  Google Scholar 

  19. Then, J. and H. G. Truper (1983) Sulfide oxidation inEctothiorhodospira abdelmalekii. Evidence for the catalytic role of cytochromec-551.Arch. Microbiol. 135: 254–258.

    Article  CAS  Google Scholar 

  20. Brume, D. C. and H. G. Truper (1986) Noncyclic electron transport in chromatophores from photolithotrophically grownRhodobacter sulfidophilis.Arch. Microbiol. 145: 295–301.

    Article  Google Scholar 

  21. Chung, Y. C., C. Huang and C. P. Tseng (1996) Biodegradation of hydrogen sulfide by a laboratory-scale immobilizedPseudomonas putida CH11 biofilter.Biotechnol. Prog. 12: 773–778.

    Article  CAS  Google Scholar 

  22. Cho, K. S., M. Hirai and M. Shoda (1992) Degradation of hydrogen sulfide byXanthomonas sp. strain DY44 isolated from peat.Appl. Environ. Microbiol. 58: 1183–1189.

    CAS  Google Scholar 

  23. Kelly, D. P. (1985) Thysiology of the thiobacilli: elucdating the sulphur oxidation pathway.Microbiol. Sci. 2: 105–109.

    CAS  Google Scholar 

  24. Kuenen, J. C. (1989) Colorless sulfur bacteria, pp. 1834–1871. In: J. T. Staley, M. P. Bryant, N. Pfenning, and J. G. Holt, (eds.),Bergey’s Manual of Systematic Bacteriology, Vol. 3, The Williams Wilkins Co., Baltimore.

    Google Scholar 

  25. Katayama-F., Y., N. Tsuzaki and H. Kuraishi (1982) Ubiquinone, fatty acid and DNA base composition determination as a guide to the taxonomy of the genusThiobacillus.J. Gen. Microbiol. 128: 1599–1611.

    Google Scholar 

  26. Takakuwa, S., T. Fujimori and H. Iwasaki (1979) Some properties of cell sulfur adhesion inThiobacillus thiooxidans.J. Gen. Appl. Microbiol. 25: 21–29.

    Article  CAS  Google Scholar 

  27. Takeuchi, T. L. and I. Suzuki (1997) Cell hydrophobicity and sulfur adhesion ofThiobacillus thiooxidans.,Appl. Environ. Microbiol. 63: 2058–2061.

    CAS  Google Scholar 

  28. Konishi, Y., S. Asai and N. Yoshida (1995) Growth kinetics ofThiobacillus thiooxidans on the surface of elemental sulfur.App. Environ. Microb. 61: 3617–3622.

    CAS  Google Scholar 

  29. Porro, S., S. Ramirez, C. Reche, C. Curutchet, A. Alonso-Romanowski, E. Donati (1997) Bacterial attachment: its role in bioleaching processes.Process Biochem. 32: 573–578

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Environmental Science and Engineering, Ewha Womans University, 120-750, Seoul, Korea

    Eun Young Lee & Kyung-Suk Cho

  2. Department of Chemical and Environmental Engineering, Soong Sil University, 156-743, Seoul, Korea

    Hee Wook Ryu

Authors
  1. Eun Young Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyung-Suk Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee Wook Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kyung-Suk Cho.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, E.Y., Cho, KS. & Ryu, H.W. Characterization of sulfur oxidation by an autotrophic sulfur oxidizer,Thiobacillus sp. ASWW-2. Biotechnol. Bioprocess Eng. 5, 48–52 (2000). https://doi.org/10.1007/BF02932353

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02932353

Keywords

Search

Navigation